IN SILICO STUDY OF MIRNA-REGULATED IQ MOTIF-CONTAINING GTPASE-ACTIVATING PROTEIN FAMILY IN LIVER CANCER


David Agustriawan, Anton Sumarpo, Arli Aditya Parikesit, Rizky Nurdiansyah, Gabriella Patricia Adisurja, Ricky Ravindra Fajar Ap

Abstract


Objective: The aim of this paper is to identify the list of microRNA (miRNA) which can regulate the aberrant expression of IQGAP in liver cancer formation. The aberrant expression of IQ motif-containing GTPase-activating protein (IQGAP) family which consists of IQGAP1, IQGAP2, and IQGAP3 has been linked to carcinogenesis in human cancers. The reciprocal expression of IQGAP family in human cancer has been studied to act as oncogenes or tumor suppressor genes. A growing number of studies suggest that upregulated or downregulated expression of IQGAP family triggers cancer development.

Methods: A correlation study was performed to construct a pathway to inhibit or activate IQGAP family between miRNAs and IQGAPs. A pre-processing step was conducted to download, filter and process the dataset from TCGA. It yields miRNA and IQGAP gene expression matrix. Then, correlation computation was computed using MATLAB. Moreover, this study linked the results to the MiRTarBase to validate the prediction result with the wet lab experimental result.

Results: This study identified significantly inversely correlation in 51 miRNAs-IQGAP1, 169 miRNAs-IQGAP2, and 33 miRNAs-IQGAP3, respectively, which may potentially play a role in a liver cancer formation. Some of the results also can be found in miRTarBase. It supports the precision of those miRNA and IQGAP interaction between dry lab and wet lab study. IQGAP1 and IQGAP2 mostly has been identified as an oncogene in cancer but IQGAP2 has been discovered as tumor suppressor gene. The list of miRNA in the result of this study can become a potential therapy to target the aberrant expression of IQGAP family.

Conclusion: miRNA function is known as an oncogene or tumor suppressor gene in cancer development. Therefore, it can be one of the important molecular biology which may target the aberrant expression of IQGAP in liver cancer.


Keywords


MicroRNA; miRNA; Oncogene; Tumor suppressor gene, IQGAP1, IQGAP2, IQGAP3, Liver cancer, Cancer.

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About this article

Title

IN SILICO STUDY OF MIRNA-REGULATED IQ MOTIF-CONTAINING GTPASE-ACTIVATING PROTEIN FAMILY IN LIVER CANCER

Keywords

MicroRNA; miRNA; Oncogene; Tumor suppressor gene, IQGAP1, IQGAP2, IQGAP3, Liver cancer, Cancer.

DOI

10.22159/ajpcr.2018.v11s3.30046

Date

06-10-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Special issue 3 2018 Page: 98-101

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

David Agustriawan
Department of Bioinformatics, Indonesia International Institute for Life Sciences, Jakarta, 13210, Indonesia.
Indonesia

Anton Sumarpo
Department of Bioinformatics, Indonesia International Institute for Life Sciences, Jakarta, 13210, Indonesia.
Indonesia

Arli Aditya Parikesit
Department of Bioinformatics, Indonesia International Institute for Life Sciences, Jakarta, 13210, Indonesia.
Indonesia

Rizky Nurdiansyah
Department of Bioinformatics, Indonesia International Institute for Life Sciences, Jakarta, 13210, Indonesia.
Indonesia

Gabriella Patricia Adisurja
Department of Bioinformatics, Indonesia International Institute for Life Sciences, Jakarta, 13210, Indonesia.
Indonesia

Ricky Ravindra Fajar Ap
Department of Bioinformatics, Indonesia International Institute for Life Sciences, Jakarta, 13210, Indonesia.
Indonesia


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