PHARMACOLOGICAL ACTIVITY OF ULVA LACTUCA POLYPHENOLS FRACTION: HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITIES AGAINST PARACETAMOL-INDUCED LIVER DAMAGE IN RATS

  • RAVINDRAN NT Department of Biochemistry, Adhiparasakthi College of Arts and Science (Autonomous), Kalavai, Tamil Nadu, India. http://orcid.org/0000-0001-6456-6992
  • MOHAMED SADIQ A Department of Biochemistry, Adhiparasakthi College of Arts and Science (Autonomous), Kalavai, Tamil Nadu, India.

Abstract

Objective: The objective of this study was to assess the activity Ulva lactuca polyphenols fraction in protecting the liver damage induced by high dose of paracetamol.


Methods: This study was performed using Wistar albino rats divided into six groups. Group 1 was the normal group. Groups 2, 3, 4, 5, and 6 received paracetamol (2 g/kg) for 7 days. In addition to paracetamol, Groups 3, 4, 5, and 6 received silymarin (100 mg/kg), U. lactuca polyphenols fraction at the doses of 50, 100, and 200 mg/kg, respectively, for 7 days. On the 8th day, serum and liver samples were collected from the animals and the hepatoprotective and antioxidant activities were assessed by studying the levels of liver marker enzymes, bilirubin, protein, reduced glutathione, and antioxidant enzymes.


Results: U. lactuca polyphenols fraction, at the tested doses, restored the levels of all serum markers and enzymes (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, γ-glutamyl transferase, total bilirubin, total protein, cholesterol, triglycerides, and reduced glutathione) and liver homogenate markers (reduced glutathione, superoxide dismutase, catalase, and glutathione peroxidase) significantly, in dose-dependent manner.


Conclusion: This study suggests that U. lactuca polyphenols fraction has a hepatoprotective effect against paracetamol-induced liver damage and possesses antioxidant activities.

Keywords: Hepatoprotective activity, Antioxidant activity, Paracetamol, Marine alga, Edible alga, Ulva lactuca, Polyphenols

Author Biographies

RAVINDRAN NT, Department of Biochemistry, Adhiparasakthi College of Arts and Science (Autonomous), Kalavai, Tamil Nadu, India.

Assistant Professor, Department of Biochemistry

MOHAMED SADIQ A, Department of Biochemistry, Adhiparasakthi College of Arts and Science (Autonomous), Kalavai, Tamil Nadu, India.

Principal and Head, Department of Biochemistry

References

1. Wolf PL. Biochemical diagnosis of liver disease. Indian J Clin Biochem 1999;14(1):59-90.
2. Anup S, Shivanandappa T. Hepatoprotective effect of the root extract of Decalepis hamiltonii against carbon tetrachloride-induced oxidative stress in rats. Food Chem 2010;118(2):411-17.
3. Ahmad I, Aqil F, Owais M. Modern Phytomedicine: Turning Medicinal Plants into Drugs. New York (NY): John Wiley & Sons; 2006.
4. Liu L, Heinrich M, Myers S, Dworjanyn SA. Towards a better understanding of medicinal uses of the brown seaweed Sargassum in traditional Chinese medicine: A phytochemical and pharmacological review. J Ethnopharmacol 2012;142:591-619.
5. Chakraborty K, Paulraj R. Sesquiterpenoids with free radical scavenging properties from marine macroalga Ulva fasciata Delile. Food Chem 2010;122:31-41.
6. Gupta S, Abu-Ghannam N. Recent developments in the application of seaweeds or seaweed extracts as a means for enhancing the safety and quality attributes of foods. Innov Food Sci Emerg Technol 2011;12:600-09.
7. Kim IH, Lee DG, Lee SH, Ha JM, Ha BJ, Kim BJ, Lee JH. Antibacterial activity of Ulva lactuca against Methicillin-resistant Staphylococcus aureus (MRSH). Biotechnol Bioprocess Eng 2007; 12:579–82.
8. Meenakshi S, Gnanambigai DM, Mozhi ST, Arumugam M, Balasubramanian, T. Total flavanoid and in vitro antioxidant activity of two seaweeds of Rameshwaram coast. Global J Pharmacol 2009; 3(2):59-62.
9. Sathivel A, Balaji Raghavendran HR, Devaki T. Hepatoprotective nature of seaweeds (Ulva lactuca/Gracilaria edulis) against liver injury induced by D-Galactosamine/endotoxin in rats. Seaweed Res Utlin 2003;25(1-2):109-11.
10. Hassan S, El-Twab SA, Hetta M, Mahmoud B. Improvement of lipid profile and antioxidant of hypercholesterolemic albino rats by polysaccharides extracted from the green alga Ulva lactuca Linnaeus. Saudi J Biol Sci 2011;18:333-40.
11. Pushparaj A, Raubbin RS, Balasankar T. Antibacterial activity of Kappaphycus alvarezii and Ulva lactuca extracts against humanpathogenic bacteria. Int J Curr Microbiol App Sci 2014;3(1):432-36.
12. Suganthy N, Karutha Pandian S, Pandima Devi K. Neuroprotective effect of seaweeds inhabiting South Indian coastal area (Hare Island, Gulf of Mannar Marine Biosphere Reserve): Cholinesterase inhibitory effect of Hypnea valentiae and Ulva reticulata. Neurosci Lett 2010;468:216–19.
13. Heo SJ, Cha SH, Lee KW, Jeon YJ. Antioxidant activities of red algae from Jeju island. Algae 2006; 21(1):149–56.
14. Ecobichon DJ. The basis of toxicology testing. 2nd ed. New York: CRC Press;1997. p 43-60.
15. Reitman S, Frankel AS. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamate pyruvate transaminase. Am J Clin Path 1957;28:53-56.
16. King EJ, Armstrong AR. Determination of serum and bile phosphatase activity. Canad Med Assoc J 1934;31:56-63.
17. King J. The hydrolase and alkaline phosphatase. In: Practical clinical enzymology. London: Nostrand Co. Ltd.;1965; p. 191.
18. Rosalki S, Rav D. Serum gamma-glutamyl transpeptidase activity in alcoholism. Clin Chem Acta 1972;39:41-47.
19. Malloy HT, Evelyn EA. The determination of bilirubin with photoelectric colorimeter. J Biol Chem 1937;119:481-85.
20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin Phenol reagent. J Biol Chem 1951;193:265-75.
21. Parekh AC, Jung DH. Cholesterol determination with ferric acetate-uranium acetate and sulfuric acid-ferrous sulfate reagents. Anal Chem 1970;42:1423-27.
22. Foster LB, Dunn RT, Stable reagents for determination of serum triglycerides by colorimetric Hantzsch condensation method. Clin Chem 1973;18:338-40.
23. Moron MS, Depierre JN, Mannervik B. Levels of glutathione, glutathione reductase and glutathione-S-transferase activity in rat lung and liver. Biochim Biophys Acta 1979;582:67–78.
24. Misra ΗP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972;247:3170-75.
25. Takahara S, Hamilton ΒΗ, Neel JV, Kobara ΤΥ, Ogura Υ, Nishimiua ΕΤ. Hypocatalasemia, a new genetic carrier state. J Clin Invest 1960;39:610-19.
26. Rortruck JT, Pope AL, Ganther HE, Swanson AB. Selenium: Biochemical roles as a component of glutathione peroxidase. Science 1973;179:588-90.
27. Larrey D. Drug induced liver disease. J Hepatol 2003;32:77-88.
28. Watkins PB, Seef LB. Drug induced liver injury: summary of a single topic clinical research committee. Hepatology 2006;43:618-31.
29. Mitra SK, Venkataranganna MV, Sundaram R, Gopumadhavan S. Protective effect of HD-03, a herbal formulation against various hepatotoxic agents in rats. J Ethnolpharmacol 1998;63:181-86.
30. Dwivedi Y, Rastogi R, Garg NK, Dhandan DM. Prevention of paracetamol-induced hepatic damage in rats by picrolir, the standardized active fraction from Picorhiza kurrao. Phytother Res 1991;5:115-19.
31. Tewari S, Gupta V, Bhattacharya S. Comparative study of antioxidant potential of tea with and without additives. Indian J Physiol Pharmacol. 2000;44:215-19.
32. Prasad VS, Venkatachalam SR, Chander R, Thomas P. Dietary antioxidants-natural defense against disease. Aryavaidyan 1999;12:149-58.
33. Bandyopadhyay U, Das D, Banerjee KR. Reactive oxygen species: Oxidative damage and pathogenesis. Curr Sci 1999;77:658-66.
Statistics
110 Views | 130 Downloads
How to Cite
RAVINDRAN NT, and MOHAMED SADIQ A. “PHARMACOLOGICAL ACTIVITY OF ULVA LACTUCA POLYPHENOLS FRACTION: HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITIES AGAINST PARACETAMOL-INDUCED LIVER DAMAGE IN RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 4, Feb. 2019, pp. 55-58, doi:10.22159/ajpcr.2019.v12i4.31214.
Section
Original Article(s)