IN VITRO GROWTH INHIBITORY EFFECT OF ZnO NANOPARTICLES ON HUMAN LIVER CANCER CELL LINES (Huh7)

  • ANANTHALAKSHMI R Department of Biochemistry, Sacred Heart College, Tirupattur, Vellore, Tamil Nadu, India.
  • XAVIER RAJARATHINAM SR Department of Chemistry, Sacred Heart College, Tirupattur, Vellore, Tamil Nadu, India.
  • Mohamed Sadiq A Adhi parasakthi college of arts and science, Kalavai, Vellore Dt
  • MOHAMED SADIQ A Department of Biochemistry, Adhiparasakthi College of Arts and Science, Vellore, Tamil Nadu, India.

Abstract

Objective: The objective of the study was to access the anticancer activity of the biosynthesized ZnO nanoparticles against Huh7 liver cancer cell lines.


Methods: The study was carried in vitro using Huh7 cell lines. The ZnO nanoparticles (ZnO NPs) were synthesized using Luffa acutangula peel extract and subjected to characterization by X-ray powder diffraction and transmission electron microscopy. The Huh7 cell lines were treated with ZnO NPs and done 3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide assay. For live and dead assay, the cell lines treated with ZnO NPs were subjected to acridine orange/ethidium (AO/ET) bromide assay.


Results: The ZnO NPs synthesized show spherical structure with 10–20 nm size. The 50% of Huh7 proliferation were inhibited at the concentration (IC50) of 40 μg/ml. The AO/ET assay shows compact nucleus and fine cytoplasmic morphology in control cells and apoptotic stage in treated cells


Conclusion: This study suggests that ZnO NPs can be prepared in environment-friendly method using aqueous extract of L. acutangula and can be used in cancer treatment effectively.

Keywords: Nanoparticles,, Eco-friendly synthesis,, Electron microscopy,, Anticancer activity,, Huh7 cell lines,, Cellular staining.

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ANANTHALAKSHMI R, XAVIER RAJARATHINAM SR, Mohamed Sadiq A, and MOHAMED SADIQ A. “IN VITRO GROWTH INHIBITORY EFFECT OF ZnO NANOPARTICLES ON HUMAN LIVER CANCER CELL LINES (Huh7)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 6, Apr. 2019, pp. 191-3, https://innovareacademics.in/journals/index.php/ajpcr/article/view/33486.
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