PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIMICROBIAL, AND ANTIBIOFILM ACTIVITY OF SAUROPUS ANDROGYNUS LEAF EXTRACTS
Objective: The objective of the study was to perform phytochemical screening, and evaluate the antioxidant, antimicrobial, and antibiofilm activities of Sauropus androgynus leaf extract against pathogenic organisms.
Methods: Sauropus androgynus leaves were shade dried, powdered, and extracted by Soxhlet hot extraction procedure using ethanol or methanol. The aqueous extract was prepared using a rotary shaker. The qualitative phytochemical screening was carried out by gas chromatography-mass spectroscopic (GC-MS) analysis and liquid chromatography-mass spectrometry (LC-MS). The antioxidant activity of plant extracts was estimated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging assay. Antimicrobial and antibiofilm activity of extracts against various pathogens was performed using serial microdilution assay and microtiter plate method, respectively.
Results: Preliminary phytochemical analysis showed the presence of compounds such as tannins, flavonoids, alkaloids, and phenols. GC-MS studies also revealed the presence of biologically active compounds. Methanolic extract of S. androgynus showed higher DPPH Scavenging activity compared to other extracts. All the extracts were able to inhibit both bacterial growth and biofilm production; however, the methanolic extract showed the highest antibiofilm activity.
Conclusion: S. androgynus plant extracts possess significant antioxidant, antimicrobial, and antibiofilm activity.
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