• SUJITHA KUTTINATH Department of Microbiology, Nehru Arts and Science College, Coimbatore – 641 105, Tamil Nadu, India.
  • HARITHA KH Department of Microbiology, Nehru Arts and Science College, Coimbatore – 641 105, Tamil Nadu, India.
  • RAM RAMMOHAN Division of Laboratory Services, Aravind Eye Hospital and Post-Graduate Institute of Ophthalmology, Coimbatore – 641 014, Tamil Nadu, India.


Objective: The objective of the study was to perform phytochemical screening, and evaluate the antioxidant, antimicrobial, and antibiofilm activities of Sauropus androgynus leaf extract against pathogenic organisms.

Methods: Sauropus androgynus leaves were shade dried, powdered, and extracted by Soxhlet hot extraction procedure using ethanol or methanol. The aqueous extract was prepared using a rotary shaker. The qualitative phytochemical screening was carried out by gas chromatography-mass spectroscopic (GC-MS) analysis and liquid chromatography-mass spectrometry (LC-MS). The antioxidant activity of plant extracts was estimated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging assay. Antimicrobial and antibiofilm activity of extracts against various pathogens was performed using serial microdilution assay and microtiter plate method, respectively.

Results: Preliminary phytochemical analysis showed the presence of compounds such as tannins, flavonoids, alkaloids, and phenols. GC-MS studies also revealed the presence of biologically active compounds. Methanolic extract of S. androgynus showed higher DPPH Scavenging activity compared to other extracts. All the extracts were able to inhibit both bacterial growth and biofilm production; however, the methanolic extract showed the highest antibiofilm activity.

Conclusion: S. androgynus plant extracts possess significant antioxidant, antimicrobial, and antibiofilm activity.

Keywords: Sauropus androgynus, Extracts, GC-MS, MIC, Biofilm.

Author Biographies

SUJITHA KUTTINATH, Department of Microbiology, Nehru Arts and Science College, Coimbatore – 641 105, Tamil Nadu, India.


HARITHA KH, Department of Microbiology, Nehru Arts and Science College, Coimbatore – 641 105, Tamil Nadu, India.


RAM RAMMOHAN, Division of Laboratory Services, Aravind Eye Hospital and Post-Graduate Institute of Ophthalmology, Coimbatore – 641 014, Tamil Nadu, India.



1. Balandrin MF, Klocke JA, Wurtele ES, Bollinger WH. Natural plant chemicals: Sources of industrial and medicinal materials. Science 1985;228:1154-60.
2. Nautiyal S, Kumar R, Hussan A. Status of medicinal plants in India, some latest issues. Ann For 2000;10:181-90.
3. Janovaska D, Kubikova K, Kokoska L. Screening for antimicrobial activity of some medicinal plants species of traditional Chinese medicine. Czech J Food Sci 2003;21:107-10.
4. Dewick PM. Tumor inhibitor from plants. Trease and Evans Pharmacognosy. Philadelphia, Pa, USA: Elsevier Health Sciences; 1996.
5. Newall CA, Anderson LA, Phillipson JD. Herbal Medicines, a Guide for Health-Care Professionals. London: The Pharmaceutical Press; 1996.
6. Magherini R. Medicinal plants aromatic yesterday and today possibility of plant breeding Agricola Litalia. Indian J Biotechnol 1998;3:136-8.
7. Patel I, Talathi A. Use of traditional Indian herbs for the formulation of shampoo and their comparative analysis. Int J Pharm Pharm Sci 2016;8:28-32.
8. Palomba EA, Semple SJ. Antibacterial activity of traditional Australian medicinal plants. J Ethnopharmacol 2001;77:151-7.
9. Moharram BA, Al-Mahbashi HM, Saif-Ali R, Ali Aqlan F. Phytochemical, anti-inflammatory, antioxidant, cytotoxic and antibacterial study of Capparis cartilaginea decne from Yemen. Int J Pharm Pharm Sci 2018;10:38-44.
10. Khan MR, Kihara M, Omoloso AD. Broad spectrum antibacterial activity of the leaves, stem and root barks of Myristica subabulata. Nat Prod Sci 2001;7:9-15.
11. Uniyal SK, Singh KN, Jamwal P, Lal B. Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya. J Ethnobiol Ethnomed 2006;2:14.
12. Archana D, Dixitha M, Santhy KS. Antioxidant and anticlastogenic potential of Piper longum L. Int J Appl Pharm 2015;7:11-4.
13. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
14. Essawi T, Srour M. Screening of some Palestinian medicinal plants for antibacterial activity. J Ethnopharmacol 2000;70:343-9.
15. Quave CL, Plano LR, Pantuso T, Bennett BC. Effects of extracts from Italian medicinal plants on planktonic growth, biofilm production and adherence of methicillin-resistant Staphylococcus aureus. J Ethnopharmacol 2008;118:418-28.
16. Sandasi M, Leonard CM, Viljoen AM. The in vitro antibiofilm activity of selected culinary herbs and medicinal plants against Listeria monocytogenes. Lett Appl Microbiol 2010;50:30-5.
17. Guarrera PM. Traditional phytotherapy in central Italy (Marche, Abruzzo, and Latium). Fitoterapia 2005;76:1-25.
18. Nahak G, Sahu RK. Free Radical Scavenging activity of multivitamin plant (Sauropus androgynous L. Merr). Researcher 2010;2:6-14.
19. Mariya P, Anto KB. Antibacterial activity of Sauropus androgynus (L.) Merr. Int J Plant Sci 2011;6:189-92.
20. Benjapak N, Swatsitang P, Tanpanich S. Determination of antioxidant capacity and nutritive values of Pak- Wanban (Sauropus androgynous L. Merr). KKU Sci J 2008;36:279-89.
21. Tonk S, Bartarya R, Maharaj Kumari K, Bhatnagar VP, Srivastava SS. Effective method for extraction of larvicidal component from leaves of Azadirachta indica and Artemisia annua linn. J Environ Biol 2006;27:103-5.
22. Handa SS, Khanuja SP, Longa G, Rakesh DD. Extraction Technologies for Medicinal and Aromatic Plants. United Nations Industrial Development Organization and the International Centre for Science and High Technology; 2008.
23. Harborne JB. Phytochemical Methods. London: Chapman & Hall; 1999. p. 60-6.
24. Hameed IH, Ibraheam IA, Kadhim HJ. Gas chromatography-mass spectrum and Fourier- transform infrared spectroscopy analysis of methanolic extract of Rosemarinus officinalis leaves. J Pharmacogn Phytother 2015;7:90-106.
25. Al-Rubaye AF, Hameed IH, Kadhim MJ. A review: Uses of gas chromatography-mass spectroscopy (GC-MS) technique for analysis of bioactive natural compounds of some plants. Int J Toxicol Pharmacol Res 2017;9:81-5.
26. NIST 11. NIST Standard Reference Database 1A. NIST Mass Spectral Search Program version 2.0g. U.S. Department of Commerce, National Institute of Standards and Technology; 2011.
27. Wiley/NIST. The Wiley/NBS Registry of Mass Spectral Data. Mass Spectral Library Version 2.0. 8th ed. New York: John Wiley & Sons, Inc., NIST, EPA, NIH; 2005.
28. Ferrer I, Thurman ME. Liquid chromatography/Time of flight/Mass spectroscopy (LC/TOF/MS) for the analysis of emerging contaminants. Trends Anal Chem 2003;22:750.
29. ChemSpider from Royal Society of Chemistry. Cambridge. Available from: [Last accessed on 2018 Jun 06].
30. Narendhirakannan RT, Nirmala JG, Caroline A, Lincy S, Saj M, Durai D. Evaluation of antibacterial, antioxidant and wound healing properties of seven traditional medicinal plants from India in experimental animals. Asian Pac J Trop Biomed 2012;2:s1245-53.
31. Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med 1998;64:711-3.
32. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disc Susceptibility Tests. Approved Standard CLSI Document. M100 S26. 27th ed. Clinical and Laboratory Standards Institute; 2017.
33. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016;6:71-9.
34. Stepanovi? S, Vukovi? D, Hola V, Di Bonaventura G, Djuki? S, Cirkovi? I, et al. Quantification of biofilm in microtiter plates: Overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS 2007;115:891-9.
35. O’Toole GA, Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: A genetic analysis. Mol Microbiol 1998;28:449-61.
36. Chaieb K, Kouidhi B, Jrah H, Mahdouani K, Bakhrouf A. Antibacterial activity of thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation. BMC Complement Altern Med 2011;11:29.
37. Huang ZR, Lin YK, Fang JY. Biological and pharmacological activities of squalene and related compounds: Potential uses in cosmetic dermatology. Molecules 2009;14:540-54.
38. Fox CB. Squalene emulsions for parenteral vaccine and drug delivery. Molecules 2009;14:3286-312.
39. Newmark HL. Squalene, olive oil, and cancer risk. Review and hypothesis. Ann N Y Acad Sci 1999;889:193-203.
40. Rao CV, Newmark HL, Reddy BS. Chemopreventive effect of squalene on colon cancer. Carcinogenesis 1998;19:287-90.
41. Lalitharani S, Mohan VR, Regini GS, Kalidass C. GC-MS analysis of ethanolic extract of Pothos scandens leaf. J Herb Med Toxicol 2009;3:159-60.
42. Soosairaj S, Dons T. Bioactive compounds analysis and characterization in ethanolic plant extracts of Justicia tranquebariensis L. (Acanthaceae) – Using GC-MS. Int J Chem Tech Res 2016;9:260-5.
43. Sujayil TK, Dhanaraj TS. Determination of bioactive compounds Evolvulus alsinoides leaf extracts using GC-MS technique. Res J Life Sci Bioinform Pharm Chem Sci 2016;2:31-8.
44. Dandekar R, Fegade B, Bhaskar VH. GC-MS analysis of phytoconstituents in alcohol extract of Epiphyllum oxypetalum leaves. J Pharmacogn Phytochem 2015;4:149-54.
45. Rao RN, Talluri MV, Krishna TS, Ravindranath K. Continuous counter current extraction, isolation and determination of solanesol in Nicotiana tobacum L. by non-aqueous reversed phase high performance liquid chromatography. J Pharm Biomed Anal 2008;46:310-5.
46. Mathavi P, Nethaji S, Velavan. GC-MS analysis of phytocomponents in the methanolic extracts of Shorea robusta. Int J Sci Res 2015;4:1935-8.
47. Selvi SV, Baskar A. Evaluation of bioactive components and antioxidant activity of Sauropus androgynus plant extracts using GC-MS analysis. Int J Pharm Sci Rev Res 2012;12:65-7.
48. Yamuna P, Abirami P, Vijayashalini P, Sharmila M. GC-MS analysis of bioactive compounds in the entire plant parts of ethanolic extract of Gomphrena decumbens. J Med Plants Stud 2017;5:31-7.
49. Raman VB, Samuel LA, Saradhi PM, Rao NB, Krishna NV, Sudhakar M, et al. Antibacterial, antioxidant activity and GC-MS analysis of Eupatorium odoratum. Asian J Pharm Clin Res 2012;5:99 106.
50. Abdullah AS, Mirghani ME, Jamal P. Antibacterial activity of Malaysian mango kernel. Afr J Biotechnol 2011;10:18739-48.
51. Ajayi GO, Olagunju JA, Ademuyiwa O, Martins OC. Gas chromatography-mass spectrometry analysis and phytochemical screening of ethanolic root extract of Plumbago zeylanica. J Med Plants Res 2011;5:1756-61.
52. Jegadeeswari P, Nishanthini A, Muthukumarasamy S, Mohan VR. GC-MS analysis of bioactive components of Aristolochia krysagathra (Aristolochiaceae). J Curr Chem Pharm Sci 2012;2:226-32.
53. Gnanadeebam SD, Viswanathan P. GC-MS analysis of phytocomponents in Spermacoce articularis L. F. leaf. Res Pharm 2015;4:1-7.
54. Samad AP, Santoso U, Chang CS, Nan FH. Identifying and analyzing bioactive components of Sauropus androgynus ethanolic leaves extract. J Fish Soc Taiwan 2013;40:257-65.
55. Saini RK, Keum YS. Tocopherols and tocotrienols in plants and their products: A review on methods of extraction, chromatographic separation and detection. Food Res Int 2016;82:59-70.
56. Parveen S, Shahzad A, Upadhyaya A, Yadav V. Gas chromatography-mass spectrometry analysis of methanolic leaf extracts of Cassia angustifolia Vahl. Asian J Pharm Clin Res 2016;9:111-6.
57. Grilo EC, Costa PN, Gurgel CS. Alpha tocopherol and gamma-tocopherol concentration in vegetable oils. Food Sci Technol 2014;34:379-85.
58. Kumar V, Yadav SK. Plant-mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 2009;84:151-7.
59. Edward PC, Varrid ET, Lynn RB. A Textbook of Pharmacognosy. London: Henry Kinston; 1970.
60. Trease GE, Evans WC. Pharmacognosy: A Physicians Guide to Herbal Medicine. 13th ed. Balleiret India, London: W.R. Saunders; 1989.
61. Akiyama H, Fujii K, Yamasaki O, Oono T, Iwatsuki K. Antibacterial action of several tannins against Staphylococcus aureus. J Antimicrob Chemother 2001;48:487-91.
62. Kumari M, Jain S. Review paper, Tannins: an anti-nutrient with positive effect to manage diabetes. Res J Recent Sci 2012;1:70-3.
63. Zhao AY. Development of natural antioxidant tea polyphenol. China Food Addit 2005;5:78-80.
64. Kumar PP, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr J Biochem Res 2010;4:191-5.
65. Andarwulan N, Batari R, Sandrasari DA, Bolling B, Wijaya H. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chem 2010;121:1231-5.
66. Badami S, Channabasavaraj KP. In vitro antioxidant activity of thirteen medicinal plants of Indian’s Western Ghats. Pharm Biol 2007;45:392-6.
67. Wong SP, Leong LP, Koh JH. Antioxidant activities of aqueous extracts of selected plants. Food Chem 2006;99:775-83.
68. Ariharan VN, Devi VN, Prasad PN. Antibacterial activity of Sauropus androgynus leaf extracts against some pathogenic bacteria. Rasayan J Chem 2013;6:134-7.
69. Wei LS, Wee W, Siong JY, Syamsumir DF. Characterization of antimicrobial, antioxidant, anticancer properties and chemical composition of Sauropus androgynus stem extracts. Acta Med Litu 2011;18:12-6.
70. Bakkiyaraj D, Pandian SK. In vitro and in vivo antibiofilm activity of a coral associated actinomycete against drug resistant Staphylococcus aureus biofilms. Biofouling 2010;26:711-7.
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How to Cite
SUJITHA KUTTINATH, HARITHA KH, and RAM RAMMOHAN. “PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIMICROBIAL, AND ANTIBIOFILM ACTIVITY OF SAUROPUS ANDROGYNUS LEAF EXTRACTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 4, Mar. 2019, pp. 244-50, doi:10.22159/ajpcr.2019.v12i4.31756.
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