TOPICAL APPLICATION OF EUGENIA CARYOPHYLLUS OIL AGAINST RINGWORM INFECTION OF HUMAN BEINGS

  • Neetu Jain SS Jain Subodh PG (Autonomous) College,
  • MEENAKSHI SHARMA Laboratory of Medical Mycology and Plant Pathology, Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India.

Abstract

Objective: At least one million people are suffering from dermatophytosis in India. These mycoses although normally not lethal are unpleasant, frequent disease recurrence, and resistant dermatophytic strains cause considerable economic losses. During the antifungal evaluation of essential oil of Eugenia caryophyllus, all concentrations were found to be an excellent inhibitor against selected fungi as compared to standard antifungal drugs griseofulvin, ketoconazole, and itraconazole. The present work deals with the preparation of an ointment from E. caryophyllus oil for the treatment of ringworm infection in human beings.


Materials and Methods: Due to these potent fungicidal properties, an ointment of E. caryophyllus was prepared and topically applied on tinea patients attending the outpatient Department of Skin, Venereology, and Leprology, SMS Hospital, Jaipur. Patients were diagnosed as tinea corporis, tinea capitis, tinea manuum, and tinea barbae. The medication was done twice a day for 3 weeks as advised by the skin specialist.


Results: All patients showed positive potassium hydroxide (KOH) results at the beginning of the trial. After the 2nd week of treatment, every patient was KOH negative and remained negative when re-examined after one month of treatment. All patients were completely cured within 3 weeks of the treatment.


Conclusions: Ointment showed excellent results, found cheaper substitutes to cure the disease without any adverse side effect. The present study offers a high possibility of complete cure of tinea infection and suggesting its uses as raw material by pharmaceutical industries for the development of antidermatophytic drug in prevailing conditions where dermatophytes are becoming resistant against popular antifungals.

Keywords: Dermatophytosis, Tinea corporis, Tinea barbae, Tinea capitis, Tinea manuum, Essential oil

References

1. Gupta BK, Kumar S, Kumar RA, Khurana S. Mycological aspects of dermatomycosis in Ludhiana. Indian J Pathol Microbiol 1993;36:233 7.
2. Patwardhan N, Dave R. Dermatomycosis in and around Aurangabad. Indian J Pathol Microbiol 1999;42:455-62.
3. Jain N, Sharma M, Sexana VN. Clinico-mycological profile of dermatophytosis in Jaipur, Rajasthan. Ind J Derma Venero Lepro 2008;74:274-5.
4. Sharma M, Sharma R. Profile of dermatophytic and other fungal infections in jaipur. Indian J Microbiol 2012;52:270-4.
5. Lohoué Petmy J, Lando AJ, Kaptue L, Tchinda V, Folefack M. Superficial mycoses and HIV infection in yaounde. J Eur Acad Dermatol Venereol 2004;18:301-4.
6. Maraki S, Tselentis Y. Dermatophytoses in crete, Greece, between 1992 and 1996. Mycoses 1998;41:175-8.
7. Hay RJ. The future of onychomycosis therapy may involve a combination of approaches. Br J Dermatol 2001;145 Suppl 60:3-8.
8. Watanabe S. Present state and future direction of topical antifungals. Nihon Ishinkin Gakkai Zasshi 1999;40:151-5.
9. Lozoya X, Navarro V, García M, Zurita M. Solanum chrysotrichum (Schldl.) a plant used in Mexico for the treatment of skin mycosis. J Ethnopharmacol 1992;36:127-32.
10. Damodaran S, Venkataraman S. A study on the therapeutic efficacy of Cassia alata, Linn. Leaf extract against pityriasis versicolor. J Ethnopharmacol 1994;42:19-23.
11. Radika SM, Michael A. In vitro antifungal activity of leaves extracts of Azadirachta indica. Int J Pharm Pharm Sci 2013;5:723-5.
12. Jain N, Sharma M. Screening of Thymus vulgaris essential oil against fungi causing dermatophytosis in human beings. Int J Pharm Pharm Sci 2017;9:236-9.
13. Jain N, Sharma M, Kumar P. Regulatory effect of some plant extracts on the growth of dermatophytic fungi. Indian J Microbiol 2004;14:56 61.
14. Jain N, Sharma M, Joshi S, Kaushik U. Toxicological aspect, chemical composition and antidermatophytic activity of essential oil of Trachyspermum ammi and their fractions. Indian J Pharm Sci 2018;80:136-42.
15. Jain N, Sharma M. Broad spectrum antimycotic drug for the treatment of ring worm infection in human beings. Curr Sci 2003;85:30-4.
16. Vanbreuseghem R. Biological technique for isolating dermatophytes from soil. Ann Soc Belg Med Trop (1920) 1952;32:173-8.
17. Wannisorn B, Jariksam S, Soontorntanasart T. Antifungal activity of lemon grass and lemon grass oil cream. Phytother Res 1996;10:551-4.
18. Provine H, Hadley S. Preliminary evaluation of a semisolid agar antifungal susceptibility test for yeasts and molds. J Clin Microbiol 2000;38:537-41.
19. Roxburgh AC, Borrie P. The English language Book Soceity. London: H.K. Kewis and Co. Ktd.; 1973.
20. Raina VK, Srivastava SK, Aggarwal KK, Syamsunder KV, Kumar S. Essential oil composition of Syzygium aromaticum leaf from little Andaman, India. Flavour Fragr J 2001;16:334-6.
21. Srivastava SK, Syamsunder KV. Bud and leaf essential oil composition of Syzygium aromaticum from India and Madagascar. Flavour Fragr J 2005;20:51-3.
22. Alma H, Murat E, Nitz S, Kollmannsberger H. Chemical composition and content of essential oil from the bud of cultivated Turkish clove (Syzygium aromaticum). Bio Resour 2007;2:265-9.
23. Mahmoud IN, Ahmed HG, Ahmed HE, Farrag AR, Shen H, Huq E, et al. Chemical constituents of clove (Syzygium aromaticum, Fam. Myrtaceae)and their antioxidant activity. Rev Latinoam Quimica 2007;35:47-57.
24. Bankar R, Kumar A, Puri S. Phytochemical constituent of Syzygium aromaticum. Int J Curr Res 2011;3:215-7.
25. Jatisatienr C, Tragoolpua K, Pank F. Effect of the Extract from Eight sps. of Medicinal Plants on Growth of Selected Plant Pathogenic Molds and Dermatophytes. Vol. 2. Proceedings, International Symposium, Breeding Research on Medicinal and Aromatic Plants. Quedlinburg, Germany, June 30-july 4, 1996. Beitrage Zur Zuctungsforchung Bundesanstalt fur Zuchtungsforchung an Kulturpflanzen; 1996. p. 414-7.
26. Bouddine L, Louaste B, Achahbar S, Chami N, Chami F, Remmal A. Comparative study of the antifungal activity of some essential oils and their major phenolic components against Aspergillus niger using three different methods. Afr J Biotechnol 2012;11:14083-7.
27. Shahi SK, Shukla AC, Bajaj AK, Banerjee U, Rimek D, Midgely G, et al. Broad spectrum herbal therapy against superficial fungal infections. Skin Pharmacol Appl Skin Physiol 2000;13:60-4.
28. Shahi KS, Shukla AC, Bajaj AK, Medgely G, Dikshit A. Broad spectrum antimycotic drug for the control of fungal infection in human beings. Curr Sci 1999;76:836-8.
Statistics
204 Views | 45 Downloads
How to Cite
Jain, N., and MEENAKSHI SHARMA. “TOPICAL APPLICATION OF EUGENIA CARYOPHYLLUS OIL AGAINST RINGWORM INFECTION OF HUMAN BEINGS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 7, May 2019, pp. 153-7, https://innovareacademics.in/journals/index.php/ajpcr/article/view/32068.
Section
Original Article(s)