• Neetu Jain University of Rajasthan Jaipur
  • Meenakshi Sharma


Objective: Lawsonia inermis belonging to family Lythreace commonly known as henna has been used in traditional herbal medicine from age. This
study deals with the extraction of henna essential oil, separation of essential oil fractions and antidermatophytic behavior of oil and their fractions.
Methods: In present investigation, essential oil obtained from the leaves of Lawsonia through hydrodistillation method was screened for their
antidermatophytic activity against selected dermatophytes through disc diffusion technique and minimal inhibitory concentration (MIC) by semisolid
agar susceptibility testing methods. Lawsonia oil was further subjected in buchii glass oven equipment for the separation of the different fraction at
different temperature interval. These fractions were labeled as LA
, LA
, LA
, LA
, and LA
Results: MIC of Lawsonia essential oil was ranging from 0.025 to 1.5 µl/ml against selected dermatophytes and other related fungi. MIC of these
fractions were also studied which were ranging from 0.3 to <4 µl/ml. Trichophyton rubrum was found to be most susceptible fungus and Candida
albicans was most resistant strain. Among all fractions studied LA
was found to be the most effective fraction. Fraction LA
was discarded because of
very less amount which could not be applied for MIC.
Conclusion: The L. inermis demonstrating broad spectra of activity may help to discover new antibiotics that could serve as selective agents for the
maintenance of animal or human health and provide biochemical tools for the study of infectious diseases. This versatile medicinal plant is the unique
source of various types of chemical compounds, which are responsible of the various activities of the plant.
Keywords: Essential oil, Dermatophytes, Trichophyton rubrum, Minimal inhibitory concentration.



Chaudhary G, Goyal S, Poonia P. Lawsonia inermis Linnaeus:

A phytopharmacological review. Int J Pharm Sci Drug Res



Anis BH, Mohamed T, Gerald C, Yves B, Samir J. Antioxidant

constituents from Lawsonia inermis leaves: Isolation, structure

elucidation and antioxidative capacity. Food Chem 2011;125:193-200.

Chetty KM. Flowering Plants of Chittoor. 1

ed. Andhra Pradesh, India:

Students Offset Printers; 2008. p. 132.


Chopra RN, Nayer SL, Chopra IC. Glossary of India Medicinal Plants.

New Delhi: CSIR Publications; 1956. p. 151.

Reddy KR. Folk medicine from Chittoor district Andhra Pradesh,

India used in the treatment of jaundice. Int J Crude Drug Res


Singh VK, Pandey DK. Fungitoxic studies on bark extract of

Lawsonia inermis against ringworm fungi. Hindusthan Antibiot Bull


Sharma VK. Tuberculostatic activity of henna Lawsonia inermis Linn.

Tubercle 1990;71(4):293-6.

Munshi SR, Shetye TA, Nair RK. Antifertility activity of three

indigenous plant preparations. Plant Med 1977;31(1):73-5.

Mohsin A, Shah AH, Al-Yahya MA, Tariq M, Tanira MO, Ageel AA.

Analgesic, antipyretic activity and phytochemical screening of

some plants used in traditional Arab system of medicine. Fitoterapia


Ali M, Grever MR. A cytotoxic napthoquinone from Lawsonia inermis.

Fitoterapia 1998;69(2):181-3.

Korayem AM, Osman HA. Nematicidal potential of the henna plant

Lawsonia inermis against the root knot nematode Meloidogyne

incognita. Anzeiger fuer Schaedlingskunde Pflanzenschutz

Umweltschutz 1992;65(1):14-6.

Singh S, Shrivastava NM, Modi NT, Saifi AQ. Anti-inflammatory

activity of Lawsonia inermis. Curr Sci 1982;51:470-1.

Jain N, Sharma M, Sharma M, Saxena VN. Spectrum of dermatophytoses

in Jaipur, India. Afr J Microbiol Res 2014;8(3):237-43.

Vanbreuseghem R. Technique biologique pour l’isolment des

dermatophytes du sol (Biological technique for the isolation of

dermatophytes from the soil). Ann Soc Belges Med Trop 1952;32:173-8.

Wannisorn B, Jariksam S, Soontorntanasart T. Antifungal activity of

lemon grass and lemon grass oil cream. Phytother Res 1996;10(7):551-4.

Patterson TF, Revankar SG, Kirkpatrick WR, Dib O, Fothergill AW,

Redding SW, et al. Simple method for detecting fluconazole-resistant

yeasts with chromogenic agar. J Clin Microbiol 1996;34(7):1794-7.

Provine H, Hadley S. Preliminary evaluation of a semisolid agar

antifungal susceptibility test for yeasts and molds. J Clin Microbiol


Rahmoun N, Boucherit-Otmani Z, Boucherit K, Benabdallah M,

Choukchou-Braham N. Antifungal activity of the Algerian Lawsonia

inermis (henna). Pharm Biol 2013;51(1):131-5.

Natarajan V, Mahendraraja S, Menon T. Antidermatophytic activities of

Lawsonia alba. Biomed 2000;20(4):243-5.

Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on 45

Indian medicinal plants against multi-drug resistant human pathogens.

J Ethanopharmacol 2001;74(2):113-23.

Raveesha KA, Satish S, Mohana DC, Raghavendra MP. Antifungal

activity of some plant extracts against important seed borne pathogens

of Aspergillus sp. J Agric Technol 2007;3(1):109-19.

Sharma KK, Saikia R, Kotoky J, Kalita JC, Devi R. Antifungal activity of

Solanum melongena L, Lawsonia inermis L. And Justicia gendarussa B.

Against dermatophytes. Int J Pharm Tech Res 2011;3(3):1635-40.

Sagar K, Vidyasagar GM. Anti-dermatophytic activity of some

traditionally used medicinal plants of North Karnataka Region. J Appl

Pharm Sci 2013;3(02):77-83.



How to Cite

Jain, N., and M. Sharma. “SCREENING OF LAWSONIA INERMIS ESSENTIAL OIL AGAINST FUNGI CAUSING DERMATOPHYTIC INFECTION IN HUMAN”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 4, July 2016, pp. 67-69,



Original Article(s)