THERAPEUTIC POTENTIAL AND IN VITRO ANTHELMINTIC ACTIVITY OF RIDGE GOURD FRUIT

  • FARES HEZAM AL-OSTOOT Department of Chemistry, Yuvaraja’s College, University of Mysore, Mysuru, India.
  • YASSER HUSSEIN EISSA MOHAMMED Department of Chemistry, Yuvaraja’s College, University of Mysore, Mysuru, India.
  • ZABIULLA Department of Chemistry, Yuvaraja’s College, University of Mysore, Mysuru, India.
  • SUNIL KUMARA DS Department of Chemistry, Yuvaraja’s College, University of Mysore, Mysuru, India.
  • SHAUKATH ARA KHANUM Department of Chemistry, Yuvaraja’s College, University of Mysore, Mysuru, India.

Abstract

Objective: The objective of the study was to evaluate the therapeutic potential and in vitro anthelmintic activity of ridge gourd fruit (Luffa acutangula) against Indian earthworms.


Methods: For anthelmintic activity against Indian earthworms (Pheretima posthuma, Ascaridia galli, and Raillietina spiralis), various different extracts concentration of L. acutangula fruit have been taken. Five concentrations as 10, 20, 30, 40, and 50 mg/ml of various extracts were tested and results were expressed in terms of time for paralysis and time for the death of worms. Albendazole (20 mg/ml) was used as reference standard and water (0.5%) as a control group.


Results: Preliminary phytochemical screening of the different extracts of ridge gourd fruit was shown to produce anthelmintic activities. In the present study, it was observed that all the extracts of ridge gourd fruit have exhibited a positive response to a certain degree of anthelmintic activity. Ethyl acetate extract exhibited more potent activity at the lower concentration of 10 mg/mL against A. galli (Roundworm). The anthelmintic activity of L. acutangula fruit extract has, therefore, been demonstrated.

Keywords: Helminth infections, Medicinal plant, Luffa Acutangula, Earthworm.

References

1. Kundu SU, Roy SA, Nandi SU, Ukil BI, Lyndem LM. In vitro anthelmintic effects of Senna occidentalis (L.) link (Leguminosae) on rat tapeworm Hymenolepis diminuta. Int J Pharm Pharm Sci 2015; 7:268-71.
2. Swargiary AN, Roy BI. In vitro anthelmintic efficacy of Alpinia nigra and its bioactive compound, astragalin against Fasciolopsis buski. Int J Pharm Pharm Sci 2015;7:30-5.
3. Enejoh SO, Suleiman MM, Ajanusi JO, Ambali SF. In vitro anthelmintic efficacy of extracts of Citrus aurantifolia (Christm) Swingle fruit peels against Heligmosomoides bakeri ova and larvae. Int J Curr Pharm Res 2015; 7:92-6.
4. MacDonald TT, Spencer J, Murch SH, Choy MY, Venugopal S, Bundy DA, et al. Immunoepidemiology of intestinal helminthic infections 3. Mucosal macrophages and cytokine production in the colon of children with Trichuris trichiura dysentery. Trans R Soc Trop Med Hyg 1994;88:265-8.
5. Patilaya PO, Husori DI, Sumantri IB. The anthelmintic effects of ethanol extract of Curanga fel-terrae leaves on Ascaridia galli. Asian J Pharm Clin Res 2017;10:117-9.
6. Coles GC. Nematode control practices and anthelmintic resistance on British sheep farms. Vet Rec 1997;141:91-3.
7. Geert S, Dorny P. Anthelmintic resistance in helminthes of animals of man in the tropics. Bull Seances Acad R Sci Outre Mer 1995;3:401-23.
8. Partap S, Kumar S, Kumar A, Sharma NK, Jha KK. In-vitro anthelmintic activity of Luffa cylindrica leaves in Indian adult earthworm. J Pharmacogn Phytochem 2012;1:27-30.
9. Sondhi SM, Sahu R, Magan A, Ghosh DK. Anti-amoebic and anthelmintic evaluation of heterocyclic compounds containing nitrogen and/or sulphur. Ind Drugs Bombay 1994;31:317-8.
10. Pimple BP, Kadam PV, Patil MJ. Antidiabetic and antihyperlipidemic activity of Luffa acutangula fruit extracts in streptozotocin induced niddm rats. Asian J Pharm Clin Res 2011;4 Suppl 2:156-63.
11. Singh H, Arora S, Mani M, Mahaur KK, Chandra P. Development of multicomponent formulation of herbal drugs for evaluation of antidiabetic activity. Der Pharm Lett 2014;6:219-23.
12. Jadhav VB, Thakare VN, Suralkar AA, Deshpande AD, Naik SR. Hepatoprotective activity of Luffa acutangula against CCl4 and rifampicin induced liver toxicity in rats: A biochemical and histopathological evaluation. Indian J Exp Biol 2010;48:822-9.
13. Misar AV, Upadhye AS, Mujumdar AM. CNS depressant activity of ethanol extract of Luffa acutangula Var. Amara CB clarke. Ind J Pharm Sci 2004;66:463-5.
14. Vanajothi R, Srinivasan P. An anthraquinone derivative from Luffa acutangula induces apoptosis in human lung cancer cell line NCI-H460 through p53-dependent pathway. J Recept Signal Transduct Res 2016; 36:292-302.
15. Manikandaselvi S, Vadivel V, Brindha P. Review on Luffa acutangula L.: Ethnobotany, phytochemistry, nutritional value and pharmacological properties. Int J Curr Pharm Rev Res 2016;7:151-5.
16. Dandge VS, Rothe SP, Pethe AS. Antimicrobial activity and pharmacognostic study of Luffa acutangula Roxb var amara on some deuteromycetes fungi. Int J Sci Innov Res 2010;2:191-6.
17. Anantharam V, Patanjali SR, Swamy MJ, Sanadi AR, Goldstein IJ, Surolia A, et al. Isolation, macromolecular properties, and combining site of a chito-oligosaccharide-specific lectin from the exudate of ridge gourd (Luffa acutangula). J Biol Chem 1986;261:14621-7.
18. Pandey VN, Malhotra SC, Sharma DP. Pharmacological Investigation of Certain Medicinal Plants and Compound Formulations Used in Ayurveda and Siddha. New Delhi, India: CCRAS; 1996. p. 20.
19. Rao BN, Rao PV, Reddy BM. Correlation and path analysis in the segregating population of ridge gourd [Luffa acutangula (Roxb.) L.]. Field Crops Res 2000;20:338-42.
20. Rao BN, Rao PV, Reddy YN. Combining ability studies in ridge gourd (Luffa acutangula (Roxb.) L.). Int J Trop Agric 2000;18:141-6.
21. Ajaiyeoba EO, Onocha PA, Olarenwaju OT. In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropsis gynandra extracts. Pharm Biol 2001;39:217-20.
22. Kumar SM, Hezam AO, Manjunath BC, Shamprasad VR, Mohammed YH, Mahesh N, et al. Crystal packing analysis of 1-(3, 4-dimethoxyphenyl)-3-(4-bromophenyl) prop-2-en-1-one exhibiting a putative halogen bond CBr...O. J Mol Struct 2018;1156:216-23.
23. Khamees HA, Mohammed YH, Swamynayaka A, Al-Ostoot FH, Sert Y, Alghamdi S, et al. Molecular structure, DFT, vibrational spectra with fluorescence effect, hirshfeld surface, docking simulation and antioxidant activity of thiazole derivative. Chem Select 2019;4:4544-58.
24. Kumara K, Al-Ostoot FH, Mohammed YH, Khanum SA, Lokanath NK. Synthesis, crystal structure and 3D energy frameworks of ethyl 2-[5-nitro-2-oxopyridine-1 (2H)-yl] acetate: Hirshfeld surface analysis and DFT calculations. Chem Data Collect 2019;20:100195.
25. Geetha DV, Al-Ostoot FH, Mohammed YH, Sridhar MA, Khanum SA, Lokanath NK. Synthesis, elucidation, hirshfeld surface analysis, and DFT calculations of 4-chloro-N-[2-(2-1H-indol-3-yl-acetylamino)- phenyl]-benzamide. J Mol Struct 2019;1178:384-93.
26. Dash GK, Suresh P, Sahu SK, Kar DM, Ganapaty S, Panda SB. Evaluation of Evolvulus alsinoides Linn. For anthelmintic and antimicrobial activities. J Nat Med 2002;2:182-5.
27. Shivkar YM, Kumar VL. Anthelmintic activity of latex of Calotropis procera. Pharm Biol 2003;41:263-5.
28. Yadav AK. Temjenmongla. Anthelmintic activity of Gynura angulosa DC. Against Trichinella spiralis infections in mice. Pharmacologyonline 2006;2:299-306.
29. Mali RG, Wadekar RR. In vitro anthelmintic activity of Baliospermum montanum muell. arg roots. Ind J Pharm Sci 2008;70:131.
30. Gbolade AA, Adeyemi AA. Investigation of in vitro anthelmintic activities of Pycnanthus angolensis and sphenocentrum jollyanum. Fitoterapia 2008;79:220-2.
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FARES HEZAM AL-OSTOOT, YASSER HUSSEIN EISSA MOHAMMED, ZABIULLA, SUNIL KUMARA DS, and SHAUKATH ARA KHANUM. “THERAPEUTIC POTENTIAL AND IN VITRO ANTHELMINTIC ACTIVITY OF RIDGE GOURD FRUIT”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 5, Apr. 2019, pp. 309-12, https://innovareacademics.in/journals/index.php/ajpcr/article/view/32777.
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