• Vasundhara Saxena
  • Garima Mishra
  • Akash Saxena
  • Kamlesh Kr. Vishwakarma


Ashoka,  arjuna  baheda  and  harde  are  constituents  of  digestive  churna  from  old  age civilization. These drugs principally contain tannins which are widespread in plant kingdom. It is a poly-phenolic compound which is proven as antioxidants and possesses a chemo- protective potential. They are found in leaves, flowers, fruits and bark. The aim of study is to estimate the amount of tannins present in these drugs using folin-denis method.Terminalia chebula (combretaceae) (harde),    Terminalia  belerica (combretaceae)(bahera), Terminalia  arjuna (combretaceae)(arjuna)  and  Saraca indica (leguminaseae) (ashoka)  were crushed to coarse powder. The alcoholic extracts of the drugs were prepared for photometric determination of tannins using folin-denis method. The tannin concentration was determined by  the  standard  graph  of  tannic  acid  solution  and  was  found  to  be  99.55456mg/gm,9.95568mg/gm, 54.96288mg/gm, and 57.4869 mg/gm for harde, arjuna, baheda and ashoka respectively.  The concentration curve for tannic acid was determined and the correlation coefficient was calculated and was found to be 0.998 which indicates the good linearity between the concentration and the absorbance. It has been found that the tannins form the stable complexes with protein, starch and metal chelates by disturbing the metabolic activity of the bacterial enzymes nutrient availability, and functionality of biological membranes. The present study can be used as one of the parameters for standardization of medicinal plants.



Download data is not yet available.


Kirtikar KR, Basu BD. Indian medicinal Plants. Lalit Mohan Basu. Allahabad, India, 1956.

Nadkarni KM, Indian Materia Medica, Bombay Prakashan, Bombay, 1982, 787.

Asolkar LV, Kakkar KK and Chakre OJ, Glossary of Indian Medicinal Plants with active principal, part-I, Publications and Information Directorate, Council of Scientific and Industrial Research Publ, New Delhi,1956, 165.

Chopra RN, Nayer SLChopra IC. Glossary of Indian Medicinal Plants. Council of Scientific and Industrial Research. New Delhi, India. 1956.

Conners, K.A., A Textbook of Pharmaceutical Analysis, 3rd Edn. A Wiley-Interscience Publication, John Wiley and Sons, New York, 1999, 595.

Despande SS, Shah GB, Parmar NS. Indian Journal of Pharmacology, 2003, 35:168–172.

Dhar ML, et al., Screening of Indian plants for biological activity, Part 1, Indian J. Exp. Biol., 2, 1968, 232-247

Jain, U.K. and Dixit, V.K., Indian Drugs, 2004, 41, 469.

Khatri A, Garg A, Agrawal SS. Evaluation of Hepatoprotective activity of areal parts of Tephrosia purpurea and Stem bark of Tecomella undulate. Journal of Ethnopharmacology. Elsevier publication, Ireland. 2009, 122, 1-5.

Peach EK, Tracy MV, Modern Methods of Plant Analysis, Springer Verlag Publication, Vol- III, 1915, 332-350.

Ram PR and Mehrotra BN, Compendium of Indian Medicinal Plants, (Drug Research Preparative: A CDRI Series), VOL. 2, Central Drug Research Institute, Lucknow and Publications and Information Directorate, New Delhi, 1993, 453.

Sane, R.T., Indian Drugs, 2002, 39, 184.

Sastri BN, The Wealth of India, Publication and Information Directorate, CSIR, Hillside, New Delhi, India,1962, 336.

Schanderl, S H (1970) In: Method in Food Analysis Academic Press New York p 709.

Michalowski, J. and P. Halaburda, 2001. Flow-injection chemiluminescence determination of epinephrine in pharmaceutical preparations using raw apple juice as enzyme source. Talanta, 55: 1165-1171.

Assimopoulou, A.N., D. Boskou, M.A. Anagnostopoulou, P. Kefalas and V.P. Papageorgiou, 2006. Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chem., 94: 19-25.

Chang, C.H., H. Lin, C.Y. Chang and Y.C. Liu, 2006. Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes. J. Food Eng., 77: 478-485.

Tanizawa, H., Y. Ohkawa, Y. Takino, A. Ueno, T. Kageyama and S. Hara, 1992. Studies on natural antioxidants in Citrus species. I. Determination of antioxidant activity of citrus fruits. Chem. Pham. Bull., 40: 1940-1942

Aruoma, O.I., 1998. Free radical, oxidative stress and antioxidant in human health and disease. J. Am. Oil Chem. Soc., 75: 199-212.

Abdi, S. and A. Ali, 1999. Role of ROS modified human DNA in the path-ogenesis and etiology of cancer. Cancer Lett., 142: 1-9.



How to Cite

Saxena, V., G. Mishra, A. Saxena, and K. K. Vishwakarma. “A COMPARATIVE STUDY ON QUANTITATIVE ESTIMATION OF TANNINS IN TERMINALIA CHEBULA, TERMINALIA BELERICA, TERMINALIA ARJUNA AND SARACA INDICA USING SPECTROPHOTOMETER”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 7, Aug. 2013, pp. 148-9, https://innovareacademics.in/journals/index.php/ajpcr/article/view/329.