COMPARATIVE STUDY AGAINST OXIDATIVE STRESS AND DNA DAMAGE PROTECTION ACTIVITY OF THE DIFFERENT EXTRACTS OF TUBERS OF ARISAEMA TORTUOSUM (WALL.) SCHOTT.

  • PRIYANKA CHAKRABORTY Department of Pharmacology, BCDA College of Pharmacy and Technology, Barasat, Kolkata, West Bengal, India.
  • NRIPENDRA NATH BALA Department of Pharmacology, BCDA College of Pharmacy and Technology, Barasat, Kolkata, West Bengal, India.
  • SUDIPTA DAS Department of Pharmaceutics, Netaji Subhas Chandra Bose Institute of Pharmacy, Chakdaha, Nadia, West Bengal, India.

Abstract

Objective: Our main aim is to evaluate the comparative in vitro antioxidant and DNA damage protection activity study with different extracts of tubers of Arisaema tortuosum.


Methods: A. tortuosum (Wall.) Schott. (Araceae family) is commonly known as whipcord cobra lily, used in India as traditional medicine. In vitro, antioxidant activity was done by six methods including determination of total phenolic and total flavonoid compounds. The DNA damage was estimated by standard diphenylamine reaction.


Results: Methanolic extract of tubers of A. tortuosum has been found rich in phenolic and flavonoid compounds when compared with other extracts. Methanolic extract of rhizomes of A. tortuosum showed potent antioxidant activity at a concentration of 400 μg/mL and potent the DNA damage protecting activity at a concentration of 50 μg/mL.


Conclusion: Comparative study against oxidative damage and protection to the DNA damage protection activity of the different extracts of tubers of A. tortuosum (Wall.) Schott. showed that methanolic extract is most potent, though ethyl acetate extract also showed activity.

Keywords: Antioxidant, DNA damage, Flavonoids, Phenolic compounds

Author Biographies

NRIPENDRA NATH BALA, Department of Pharmacology, BCDA College of Pharmacy and Technology, Barasat, Kolkata, West Bengal, India.

Prof.(Dr.)Nripendra Nath Bala, Principal

SUDIPTA DAS, Department of Pharmaceutics, Netaji Subhas Chandra Bose Institute of Pharmacy, Chakdaha, Nadia, West Bengal, India.
Assoc. Professor
B.Pharm., M.Pharm., PhD

References

1. Cheeseman KH, Slater TF. Free radicals in medicine. Br Med Bull 1993;49:479-724.
2. Madamanchi NR, Vendrov A, Runge MS. Oxidative stress and vascular disease. Arterioscler Thromb Vasc Biol 2005;25:29-38.
3. Panchawat S, Rathore KS, Sisodia SS. A review on herbal antioxidants. Int J PharmTech Res 2010;2:232-9.
4. Verma H, Lal VK, Pant KK, Soni N. An ethnomedicinal review on Arisaema tortuosum. Int J Adv Pharm Biol Chem 2012;1:176-9.
5. Alidadi S, Moradi MT, Asadi-Samani M, Lorigooini Z. Antioxidant potential and total phenolic compounds of extracts and fractions of Pistasia atlantica. Int J Pharm Clin Res 2017;9:293-7.
6. Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, et al. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol 2000;72:35-42.
7. Jebitta R, Allwin J. Antioxidant activity, total phenolic, flavonoid, and anthocyanin contents of jamun (Syzygium cumini) pulp powder. Asian J Pharm Clin Res 2016;9:361-3.
8. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26:1231-7.
9. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebens Wiss Technol 1995;28:25-30.
10. Halliwell B, Gutteridge JM, Aruoma OI. The deoxyribose method: A simple “test-tube” assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 1987;165:215-9.
11. Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr 1986;44:307-15.
12. Turrens JF. Superoxide production by the mitochondrial respiratory chain. Biosci Rep 1997;17:3-8.
13. Burton K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 1956;62:315-23.
14. McCord JM. The evolution of free radicals and oxidative stress. Am J Med 2000;108:652-9.
15. Scherer R, Godoy HT. Antioxidant activity index (AAI) by the 2, 2-diphenyl-1-picrylhydrazyl method. Food Chem 2009;112:654-8.
16. Puri SK, Habbu PV, Kulkarni PV, Kulkarni VH. Characterization, in vitro antioxidant and hepatoprotective activity of fungal endophytic extracts of Andrographis paniculata leaves in CCL4 induced hepatotoxicity. Int J Pharm Pharm Sci 2019;11:44-54.
17. Nwakaego NL, Chibuike OK, Chukwugekwu EM, Marylyn AC, Ngozi EI, Chukwunonye ER. In vitro antioxidant and free radical scavenging potential of methanolic extracts of Uvaria chamae leaves and roots. Int J Pharm Pharm Sci 2019;11:67-71.
18. Shahidi F, Wanasundara PK. Phenolic antioxidants. Crit Rev Food Sci Nutr 1992;32:67-103.
19. Hatano T, Edamatsu R, Mori A. Effect of interaction of tannins and related poyphenols on superoxide anion radical and on DPPH radical. Chem Pharm Bull 1989;37:2016-21.
20. Umamaheswari M, Chatterjee TK. In vitro antioxidant activities of the fractions of coccinia grandis L. leaf extract. Afr J Tradit Complement Altern Med 2007;5:61-73.
21. Cao G, Sofic E, Prior RL. Antioxidant and prooxidant behavior of flavonoids: Structure-activity relationships. Free Radic Biol Med 1997;22:749-60.
22. Wu LC, Hsu HW, Chen YC, Chiu CC, Lin YI, Ho JA. Antioxidant and antiproliferative activities of red pitaya. Food Chem 2006;95:319-27.
23. Silva CG, Herdeiro RS, Mathias CJ, Panek AD, Silveira CS, Rodrigues VP, et al. Evaluation of antioxidant activity of Brazilian plants. Pharmacol Res 2005;52:229-33.
24. Pan Y, Wang K, Huang S, Wang H, Mu X, He C, et al. Antioxidant activity of microwave-assisted extract of longan (Dimocarpus longan Lour.) peel. Food Chem 2008;106:1264-70.
25. Panda S, Chakraborty M, Majumder P, Mazumder S, Das S, Haldar PK. Antidiabetic, antioxidant and anti-hyperlipidaemic activity of Cucumis callosus in streptozotocin-induced diabetic rats. Int J Pharm Sci Res 2016;7:1978-84.
26. Lu Y, Foo LY. Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chem 2001;75:197-202.
27. Klein SM, Cohen G, Cederbaum AI. Production of formaldehyde during metabolism of dimethyl sulfoxide by hydroxyl radical generating systems. Biochemistry 1981;20:6006-12.
28. Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. Antioxidative activity and safety of the 50 ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1. J Agric Food Chem 2002;50:2454-8.
29. Chanda S, Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. Afr J Microbiol Res 2009;13:981-96.
30. Chakraborty M, Karmakar I, Haldar S, Das A, Bala A, Haldar PK. Amelioration of oxidative DNA damage in mouse peritoneal macrophages by Hippophae salicifolia due to its proton (H(+)) donation capability: Ex vivo and in vivo studies. J Pharm Bioallied Sci 2016;8:210-6.
Statistics
201 Views | 114 Downloads
Citatons
How to Cite
PRIYANKA CHAKRABORTY, NRIPENDRA NATH BALA, and S. DAS. “COMPARATIVE STUDY AGAINST OXIDATIVE STRESS AND DNA DAMAGE PROTECTION ACTIVITY OF THE DIFFERENT EXTRACTS OF TUBERS OF ARISAEMA TORTUOSUM (WALL.) SCHOTT.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 1, Nov. 2019, pp. 166-70, doi:10.22159/ajpcr.2020.v13i1.36284.
Section
Original Article(s)