• Siva Prasad Panda Jadavpur University
  • Pallab Kanti Haldar
  • Sudipta Das


Objective: This research investigated in vitro hypoglycemic and antimicrobial activity of methanolic fruit extract of Cucumis callosus (MECC) (Rottl.)
Methods: The in vitro antidiabetic ability of MECC was evaluated by inhibition studies of the digestive enzymes including α-amylase and α-glycosidase.
The antimicrobial activity of extract was further evaluated against 21 laboratory strains belongs to 8 bacterial species and 4 fungal species using cupplate
diffusion assay
and agar
dilution assay.
Results: Our assay result suggested that the MECC exhibited dose-dependent increase in percentage inhibitory activity on α-glycosidase
405.37 µg/ml) and α-amylase enzymes (IC
721.55 µg/ml). The results of antimicrobial activity also showed that the extract inhibited the growth
of 13 strains of 7 bacterial species and all fungal species.
Conclusion: The observed inhibitions of α-amylase and α-glycosidase and inhibition of some microbial species suggested that the MECC may be useful
in the management of diabetes mellitus and treatment of some infectious diseases.
Keywords: Cucumis callosus, Diabetes, α-amylase, α-glycosidase, Antimicrobial.

Author Biography

Siva Prasad Panda, Jadavpur University
research scholar, dept. of Pharm Tech., Jadavpur University


1. Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of
diabetes for 2010 and 2030. Diabetes Res Clin Pract 2010;87:4-14.
2. Tripathy KD. Insulin, oral hypoglycaemic drugs and glucagon.
Essential Medical Pharmacology. 6
S. aureus ML 351 G+ 14 15 28 31
S. aureus ML 314 G+ 13 16 34 38
S. aureus ML 264 G+ 13 15 21 28
S. aureus ML 267 G+ 12 12 30 34
B. subtilis UC 564 G+ 10 12 18 25
B. pumilus 8241 G+ 8 11 17 26
B. cereus PI 11778 G+ 10 12 14 20
V. cholerae 117 G− 12 13 24 32
K. pneumoniae 114 G− 12 14 27 30
E. coli DH 5α G− 10 11 34 38
S. dysenteriae 7 G− 13 15 24 30
S. boydi RC G− 18 20 33 34
S. typhimurium 74 G− 10 12 17 30
C. albicans MTCC 183 10 16 34
A. flavus MTCC 1973 15 17 30
A. niger MTCC 16404 15 16 30
C. albidus MTCC 183 17 18 28
Values are zone of inhibition (mm, including the diameter of the bore [6 mm]). Penicillin (100 µg/ml), chloramphenicol (100 µg/ml). Griseofulvin (100 µg/ml).
MECC: Methanolic fruit extract of Cucumis callosus, ATCC: American Type Culture Collection, MTCC: Microbial Type Culture Collection. S. aureus: Staphylococcus aureus,
B. subtilis: Bacillus subtilis, B. pumilus: Bacillus pumilus, B. cereus: Bacillus cereus, V. cholera: Vibrio cholera, K. pneumoniae: Klebsiella pneumoniae, E. coli: Escherichia coli,
S. dysenteriae: Shigella dysenteriae, S. boydi: Shigella boydi, S. choleraesuis: Salmonella choleraesuis, S. typhimurium: Salmonella typhimurium, P. vulgaris: Proteus vulgaris,
P. aeruginosa: Pseudomonas aeruginosa, C. albicans: Candida albicans, A. flavus: Aspergillus flavus, A. niger: Aspergillus niger, C. albidus: Cryptococcus albidus
ed. New Delhi: Jaypee Brother’s
Medical Publishers (P) ltd.; 2008. p. 255-74.
3. Bhalodi M, Shukla S, Saluja AK. In vitro antioxidant activity of the
flowers of Ipomoea aquatic Forsk. Pharmacogn Mag 2008;4:220-6.
4. Ali H, Houghton PJ, Soumyanath A. Alpha-Amylase inhibitory activity
of some Malaysian plants used to treat diabetes; With particular
reference to Phyllanthus amarus. J Ethnopharmacol 2006;107:449-55.
5. Rathore M. Nutrient content of important fruit trees from arid zone of
Rajasthan. J Hortic Forestry 2009;1(7):103-8.
6. Madhav R. Glimpses of Nature Series (No.4): Our Monsoon Plants.
Bombay: Bombay Natural History Society; 1959. p. 5.
7. Singh DK. Cucurbits. In: Peter KV, editor. Underutilized and
Underexploited Horticulture Crops. Vol. 2. New Delhi: New India
Publishing Agency; 2007. p. 175-93.
8. Kirtikar KR, Basu BD. Indian Medicinal Plants. 2
ed., Vol. 1.
Allahabad, India: Lalit Mohan Basu Academy; 1984. p. 1585-8.
9. Rahman AH. Ethno-medicinal investigation on ethnic community in
the Northern region of Bangladesh. Am J Life Sci 2013;1(2):77-81.
10. Patel PK, Parekh PP, Sorathia KD. Studies on ethnomedicinal aspects
of family cucurbitaceae in North Gujarat. UJPBS 2013;1(1):34-6.
11. Ediriweera ER, Ratnasooriya WD. A review on an herb used in
treatment of diabetes mellitus by Sri Lankan Ayurvedic and traditional
physicians. AYU 2009;30(4):373-91.
12. Chand T, Bhandari A, Bhupendra KK, Sharma A, Pareek A, Bansal VK.
In vitro antioxidant activity of aqueous extract of seeds of Cucumis
callosus (Rottl.) Cogn. Der Pharm Lett 2012;4(3):840-4.
13. Chand T, Bhandari A, Kumawat BK, Basniwal PK, Sharma S, Verma R.
Panda et al.
Asian J Pharm Clin Res, Vol 9, Suppl. 2, 2016, 77-81
In vitro antioxidant activity of alcoholic extract of seeds of Cucumis
callosus (Rottl.) Cogn. Am J Pharm Tech Res 2012;2(3):986-92.
14. Gill NS. Review of Cucumis callosus. Glob J Pharm Res
15. Heidari R, Zareae S, Heidarizadeh M. Extraction, purification, and
inhibitory effect of alpha-amylase inhibitor from wheat (Triticum
aestivum Var. Zarrin). Pak J Nutr 2005;4(2):101-5.
16. Kim JS, Kwon CS, Son KH. Inhibition of alpha-glucosidase and
amylase by luteolin, a flavonoid. Biosci Biotechnol Biochem
17. NCCLS. Methods for dilution antimicrobial susceptibility tests for
bacteria that grow aerobically-Approved standard M7-A5. 5
Villanova, PA: NCCLS; 2000.
18. Mahesh B, Satish S. Antimicrobial activity of some important
medicinal plant against plant and human pathogens. World J Agric Sci
19. Ainsworth EA, Gillespie KM. Estimation of total phenolic content and
other oxidation substrates in plant tissues using folin–ciocalteu reagent.
Nat Protoc 2007;2(4):875-7.
20. Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the
free radical-scavenging activity of propolis from several regions of
Argentina. J Ethnopharmacol 2000;71(1-2):109-14.
21. Ranilla LG, Kwon YI, Apostolidis E, Shetty K. Phenolic compounds,
antioxidant activity and in vitro inhibitory potential against key
enzymes relevant for hyperglycemia and hypertension of commonly
used medicinal plants, herbs and spices in Latin America. Bioresour
Technol 2010;101:4676-89.
22. Tundis R, Loizzo MR, Menichini F. Natural products as alphaamylase
and alpha-glucosidase inhibitors and their hypoglycaemic

in the treatment of diabetes:
update. Mini Rev Med

23. Nair SS, Kavrekar V, Mishra A. In-vitro studies on alpha amylase and
alpha glycosidase inhibitory activities of selected plant extracts. Eur J
Exp Biol 2013;3(1):128-32.
24. Sales PM, Souza PM, Simeoni LA, Silveira D. A-Amylase inhibitors:
A review of raw material and isolated compounds from plant source.
J Pharm Pharm Sci 2012;15:141-83.
25. Darah I, Lim CL, Nurul AZ, Nor AS, Shaida FS. Effects of
methanol extract of a soft sponge, sp. On bacterial cells: Structural
degenerationstudy. Pharm Glob Int J Compr Pharm 2011;2:1-6.
26. Supardy NA, Ibrahim D, Sulaiman SF, Zakaria NA. Inhibition of
Klebsiella pneumoniae ATCC 13883 cells by hexane extract of
Halimeda discoidea (Decaisne) and the identification of its potential
bioactive compounds. J Microbiol Biotechnol 2012;22:872-81.
27. Korir RC, Mutai C, Kiiyukia C, Bii C. Antimicrobial activity and safety
of two medicinal plants traditionally used in Bomet district of Kenya.
Res J Med Plant 2012;6(5):370-82.
28. Pagès JM, James CE, Winterhalter M. The porin and the permeating
antibiotic: A selective diffusion barrier in gram-negative bacteria. Nat
Rev Microbiol 2008;6:893-903.
29. Rhouma A, Ben Daoud H, Ghanmi S, Ben Salah H, Romdhane M,
Demak M. Antimicrobial activities of leaf extracts of Pistacia and
Schinus species against some plant pathogenic fungi and bacteria. J
Plant Pathol 2009;91(2):339-45.
30. Dahiya P, Purkayastha S. Phytochemical screening and antimicrobial
activity of some medicinal plants against multi-drug resistant bacteria
from clinical isolates. Indian J Pharm Sci 2012;74(5):443-50.
31. Pushpahasni K, Sharmila RS, Dhasarathan P. Efficiency of in-vitro
antibacterial activity of Syzygium cumini phenolic extract from leaves.
Asian J Pharm Clin Res 2015;8(6):211-4.
32. Bidarigh S, Massiha A, Pahlaviani MR, Issazadeh K, Muradov PZ,
Azarpour E. Antimicrobial (screening) properties of various plant
extracts from Ocimum basilicum L. And Nerium oleander L. Against
fungal common rots of potato In vitro bioassay. J Basic Appl Sci Res
33. Al-daihan S, Bhat RS. Antibacterial activities of extracts of leaf,
fruit, seed and bark of Phoenix dactylifera. Afr J Biotechnol
334 Views | 292 Downloads
How to Cite
Panda, S. P., P. Kanti Haldar, and S. Das. “IN VITRO HYPOGLYCEMIC AND ANTIMICROBIAL ACTIVITY OF CUCUMIS CALLOSUS (ROTTL.) COGN. FRUIT”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 8, Oct. 2016, pp. 77-81, doi:10.22159/ajpcr.2016.v9s2.11687.
Original Article(s)