EVALUATION OF ANTI CANCER POTENTIAL OF METHANOL EXTRACT OF CURCUMA ZEDOARIA.

  • Prosanto Pal Himalayan Pharmacy Institute
  • Arti Kumari Prasad Himalayan Pharmacy Institute
  • Mainak Chakraborty Jadavpur University
  • Sagnik Haldar Jadavpur University
  • Poulami Majumder Jadavpur University
  • Pallab Kanti Haldar Jadavpur University

Abstract

Objective: Evaluation of anti cancer activity of methanol extracts of Curcuma zedoaria against Ehrlich’s ascities carcinoma (EAC) cell line in Swiss
albino mice.
Method: In vitro cytotoxicity assay has been evaluated by using the trypan blue and MTT assay method. In vivo anti cancer activity was performed
by using EAC cells induced mice groups (n=12), at the doses of 100 and 200 mg/kg b.w. respectively, half of the mice were sacrificed and the rest
were kept alive for life span parameter. The anti cancer potential of MECZ was assessed by evaluating tumor volume, viable and nonviable tumor
cell count, tumor weight, hematological parameters and biochemical estimations. Furthermore, antioxidant parameters were assayed by estimating
liver tissue enzymes.
Result: Dose dependent cytotoxicity was observed in (* p < 0.05) Trypan blue and MTT assay method. In vivo anti cancer parameters like tumor
volume, tumor weight, and viable cell count were decreased compared to the EAC control group. MECZ treated EAC cell–bearing mice had an
increased life span to that of EAC control group. Hematological and serum biochemical profiles were restored to normal levels in MECZ-treated mice
compared to the EAC control group. Among the tissue parameters lipid peroxidation, reduced glutathione,superoxide dismutase, and catalase
toward normal levels compared to the EAC control group. In short,
Conclusion: MECZ exhibited remarkable antitumor activity in Swiss albino mice, which is attributed to its augmentation of endogenous antioxidant
activities and cytotoxic nature.
Keywords: Curcuma zedoaria, Zingiberaceae, EAC cell line, antitumor activity, 5-Flurouracil

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Pal, P., A. K. Prasad, M. Chakraborty, S. Haldar, P. Majumder, and P. K. Haldar. “EVALUATION OF ANTI CANCER POTENTIAL OF METHANOL EXTRACT OF CURCUMA ZEDOARIA.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 271-5, https://innovareacademics.in/journals/index.php/ajpcr/article/view/7419.
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