EVALUATION OF ANTI CANCER POTENTIAL OF METHANOL EXTRACT OF CURCUMA ZEDOARIA.
Objective: Evaluation of anti cancer activity of methanol extracts of Curcuma zedoaria against Ehrlichâ€™s ascities carcinoma (EAC) cell line in Swiss
Method: In vitro cytotoxicity assay has been evaluated by using the trypan blue and MTT assay method. In vivo anti cancer activity was performed
by using EAC cells induced mice groups (n=12), at the doses of 100 and 200 mg/kg b.w. respectively, half of the mice were sacrificed and the rest
were kept alive for life span parameter. The anti cancer potential of MECZ was assessed by evaluating tumor volume, viable and nonviable tumor
cell count, tumor weight, hematological parameters and biochemical estimations. Furthermore, antioxidant parameters were assayed by estimating
liver tissue enzymes.
Result: Dose dependent cytotoxicity was observed in (* p < 0.05) Trypan blue and MTT assay method. In vivo anti cancer parameters like tumor
volume, tumor weight, and viable cell count were decreased compared to the EAC control group. MECZ treated EAC cellâ€“bearing mice had an
increased life span to that of EAC control group. Hematological and serum biochemical profiles were restored to normal levels in MECZ-treated mice
compared to the EAC control group. Among the tissue parameters lipid peroxidation, reduced glutathione,superoxide dismutase, and catalase
toward normal levels compared to the EAC control group. In short,
Conclusion: MECZ exhibited remarkable antitumor activity in Swiss albino mice, which is attributed to its augmentation of endogenous antioxidant
activities and cytotoxic nature.
Keywords: Curcuma zedoaria, Zingiberaceae, EAC cell line, antitumor activity, 5-Flurouracil
unbalances splenic cell populations and reduced
responsiveness of T cells to Staphylococcus aureusenterotoxin
B stimulation. Immunomol Lett 2000 ; 74:111â€“5.
2. Muthukumar T, Christyn A, Mangadu A, Malaisamy M, Sivaraj C,
Balasubramanian K. Anticancer and antioxidant activity of
Curcuma zedoaria and Curcuma amada rhizome extracts. J.
Acad. Indus. Res 2012; 1(2) : 91-6.
3. Rahman A, Afroz M, Islam R, Islam K, Hossain M. In vitro
antioxidant potential of the essential oil and leaf extracts of
Curcuma zedoaria Rosc. J App Pharm Sci 2014; 4 (2): 107-111.
4. Lai CYE, Chycu CC, Mau LJ, Chen CC, Lai JY, Shih FC, Lin LL.
Antimicrobial activity and cytotoxicity of the essential oil of
Curcuma zedoaria. Am J Chin Med 2004; 32(2): 281â€“90.
5. OECD. Guidelines for the testing of chemicals/section 4: Health
effects test No. 425: Acute oral toxicity: up-and-down
procedure. Paris: Organisation for economic co-operation and
6. Karmakar I, Dolai N, Kumar RBS, Kar B, Roy SN, Haldar PK.
Antitumor activity and antioxidant property of Curcuma caesia
against Ehrlichâ€™s ascites carcinoma bearing mice. Pharm Biol
7. Haldar S, Karmakar I, Chakraborty M, Ahmad D, Haldar PK.
Antitumor potential of Thevetia peruviana on Ehrlichâ€™s ascites
carcinomaâ€“Bearing Mice.J Enviorn Pathol Toxicol Oncol 2015;
8. Bala A, Kar B, Haldar PK, Majumder UK, Bera S. Evaluation of
anticancer activity of Cleome gynandra on Ehrlichâ€™s ascites
carcinoma treated mice. J Ethnopharmacol 2010;129;131â€“4.
9. Dolai N, Karmakar I, Kumar RBS, Kar B, Bala A, Haldar PK.
Evaluation of antitumor activity and in vivo antioxidant status
of Anthocephalus cadamba on Ehrlich ascites carcinoma treated
mice. J Ethnopharmacol 2012;142: 865â€“70.
10. Saha P, Mazumder UK, Haldar PK, Naskar S, Kundu, S., Bala A,
Kar B. Anticancer activity of methanol extract of Cucurbita
maxima against Ehrlich ascites carcinoma. Int. J. Res. Pharm. Sci
2011; 2(1): 52-59.
11. Kumar RBS, Kar B, Dolai N, Karmakar I, Haldar S, Bhattacharya
S, Haldar PK. Antitumor activity and antioxidant role of Streblus
asper bark aganist Ehrlichâ€™s ascites carcinoma in swiss albino
mice. J Exp Ther Oncology 2013; 10(3):197â€“202.
12. Kathiriya K, Das EP, Kumar KB. Mathai evaluation of antitumor
and antioxidant activity of Oxalis corniculata Linn. against
Ehrlich ascites carcinoma on mice. Iran J Cancer Prev 2010;
Haldar et al.
Asian J Pharm Clin Res, Vol 7, Issue 5, 2015, 309-313
Toxicol Environ Chem. 2010; 92: 1
13. Haldar PK, Kar B, Bala A, Bhattacharya S, Mazumder UK.
Antitumor activity of Sansevieria roxburghiana rhizome against
Ehrlichâ€™s ascites carcinoma in mice. Pharm Biol 2010;48: 1337â€“
14. Yagi K. Lipid peroxides and human diseases. Chem Physiol Lip
15. Louw DF, Bose R, Sima AA. TBARS, Createnin and reduced
glutathione level in human. Neurosurgery 1997; 41: 1146-50.
16. Rushmore TH, Picket CB. Glutathione-S-transferase, structure,
regulation and therapeutic implication. J Biol Chem. 1993; 268:
17. Haldar PK, Bhattacharya S, Kar B, Bala A, Mazumder UK.
Chemopreventive role of Indigofera aspalathoides in 20methylcholanthrene-induced
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