TOXICITY PROFILE OF CELASTRUS PANICULATUS SEEDS: A PRECLINICAL STUDY
Objective: The objective of the study was to evaluate the toxicity profile of Celastrus paniculatus (CP) by performing a preclinical study on Swiss albino mice and demonstrate a safety description through monitoring their autonomic, neurological, behavioral, physical, and biochemistry profiles.
Methods: The toxicity profiles (acute and subacute) of CP were evaluated using Swiss albino mice in which they were divided into four groups: Group I received 1% Tween 20 and dimethyl sulfoxide. Group II, III, and IV received CP seed oil orally, at doses of 300, 2000, and 5000 mg/kg body weight for both acute and subacute toxicity studies in accordance with Organization for Economic Cooperation and Development guidelines No. 423. Special attention was given during the first 4 h and daily thereafter for a total of 14 days. Behavioral profile, physical state changes, and other parameters such as tremors, convulsion, lethargy were noted. Clinical signs were observed daily during the 28 days of the treatment period. Body weights were measured once a week. On the 29th day, the animals were kept to overnight and blood samples were collected through retro-orbital puncture for biochemical analysis.
Results: In both acute and subacute toxicity studies, the treatment with CP did not affect the normal health status of animals. It is suggestive that CP is considered practically non-toxic.
Conclusion: The toxicity profile of CP seed oil was evaluated and found to be safe until 2000 mg/kg dose.
2. Younus M. Ethno botanical study and traditional uses of Celastrus paniculatus. Int J Innov Sci Eng Technol 2015;2:139-43.
3. Bhanumathy M, Chandrashekhar SB, Somasundaram T. Phytopharmacology of Celastrus paniculatus: An overview. Int J Pharm Sci Drug Res 2010;2:176-81.
4. Godkar PB, Gordon RK, Ravindran A, Doctor BP. Celastrus paniculatus seed water soluble extracts protect against glutamate toxicity in neuronal cultures from rat forebrain. J Ethnopharmacol 2004;93:213-9.
5. Valecha R, Dhingra D. Behavioral and biochemical evidences for antidepressant-like activity of Celastrus paniculatus seed oil in mice. Basic Clin Neurosci 2016;7:49-56.
6. Kaur D, Shri R, Kamboj A. Evaluation of anti-anxiety effect of Brassica oleracea L. extracts in experimental animals. Pharmacogn J 2017;9:638-43.
7. Bhagya V, Christofer T, Shankaranarayana Rao BS. Neuroprotective effect of Celastrus paniculatus on chronic stress-induced cognitive impairment. Indian J Pharmacol 2016;48:687-93.
8. Borrelli F, Borbone N. Potent relaxant effect of a Celastrus paniculatus extract in the rat and human ileum. J Ethnopharmacol 2009;122:434-8.
9. Lekha G, Mohan K, Samy IA. Effect of Celastrus paniculatus seed oil (Jyothismati oil) on acute and chronic immobilization stress induced in swiss albino mice. Pharmacogn Res 2010;2:169-74.
10. Sharwan G, Jain P, Pandey R, Shukla SS. Toxicity profile of traditional herbal medicine. J Ayu Herb Med 2015;1:81-90.
11. Thomson J. Handbook of Radioactivity Analysis. 3rd ed. San Diego, CA: Academic Press; 2012.
12. Mehta RM. Textbook of Pharmaceutics.1st ed. New Delhi: Vallabh Prakashan Publishers; 1997.
13. Lalitha P, Shuashini KS, Jayanthi P. Acute toxicity study of extracts of Eichhornia crassipes (Mart). Solms. Asian J Pharm Clin Res 2012;5:59-61.
14. Organisation for Economic Co-operation and Development. OECD Guideline for Testing of Chemicals. Acute Oral Toxicity-Acute Toxic Class Method 423 Adopted. Paris: Organisation for Economic Co-operation and Development; 2001. p. 1-14.
15. Estuningtyas A, Widiasari S, Kusmardi K. Acute toxicity of chitosan nanoparticles containing mahkota dewa (Phaleria macrocarpa) leaf extract and anti-inflammatory effects in a dextran sodium sulfate-induced mouse model of ulcerative colitis. Int J Appl Pharm 2018;10:6-10.
16. Shivaraj YN, Govind SR, Jogaiah S, Sannaningaiah D. Functional analysis of medicinal plants using systems biology approaches. Int J Pharm 2015;7:41-3.
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