• ALEESHA R Department of Pharmacology and Research and Development Cell, Nirmala College of Pharmacy, Muvattupuzha, Ernakulam, Kerala, India.
  • BHARAT MISHRA Department of Pharmacology and Research and Development Cell, Nirmala College of Pharmacy, Muvattupuzha, Ernakulam, Kerala, India.


Objectives: The present study was envisaged to identify the effect of anti-inflammatory and antidiabetic activity for methanolic bark extracts of Simarouba glauca.

Methods: The present study design was to evaluate the in vitro anti-inflammatory and in vitro antidiabetic activity of S. glauca methanolic bark extract. To examine the antidiabetic activity, the samples were studied for their effect on inhibition of alpha-amylase and glucose transport across the dialysis membrane. In vitro anti-inflammatory activity was evaluated using albumin denaturation assay and membrane stabilization method.

Results: Our current results indicate that the various bioactive constituents detected in S. glauca may be responsible for its in vitro antidiabetic and anti-inflammatory effects. The ability of plant extract on anti-inflammatory activity showed that it was effective in inhibiting heat-induced albumin denaturation with an IC50 value of test and standard was found to be 46.42 μg/ml and 24.09 μg/ml. In addition to this, heat-induced hemolysis was also performed. The IC50 values of the test and standard were found to be 43.51 μg/ml and 21.41 μg/ml, respectively. The percentage inhibition of the test sample varied from the concentration range of 75 to 100 μg/ml. The IC50 value of the test and standard was found to be 19.08 μg/ml and 9.08 μg/ml, respectively.

Conclusion: The findings of the present study concluded that S. glauca bark has the potential to treat diabetes and a novel natural anti-inflammatory agent as a good source. Thus, S. glauca may be a potential candidate for the development of future antidiabetic and anti-inflammatory compounds. However, still further studies and standardization of the plant research may be required to develop them as medicine.

Keywords: Simarouba glauca, Alpha-amylase, Albumin denaturation, Membrane stabilization, Heat-induced hemolysis


1. Leelaprakash G, Mohan DS. In-vitro anti-inflammatory activity of methanol extract of Enicostema axillare. Int J Drug Dev Res 2010;3:189-96.
2. El-Gabalawy H, Guenther LC, Bernstein CN. Epidemiology of immune-mediated inflammatory diseases: Incidence, prevalence, natural history, and comorbidities. J Rheumatol Suppl 2010;1:2-10.
3. Hussain MS, Hiremath MB. Evaluation of in vitro antioxidant and anti-inflammatory activities of Aegle marmelos leaf extracts. Asian J Pharm Clin Res 2020;13:209-13.
4. Okechukwu PN. Evaluation of anti-inflammatory, analgesic, antipyretic effect of eicosane, pentadecane, octacosane, and heneicosane. Asian J Pharm Clin Res 2020;13:29-35.
5. Solomon MA, Shtay AB. The effect of supervised progressive resistance training (PRT) on glycemic control and cardio vascular disease (CVD) risk markers in Type 2 diabetes patients, North West Ethiopian. J Diabetes Metab 2012;3:2-5.
6. Switi BG, Mohan GK, Rani MS. Phytochemicals for diabetes management. Pharm Crops 2014;5 Suppl 1:11-28.
7. Kumar AB, Umashankar MS. A case study on clinical pharmacist interventions in the management of diabetes complicated coronary artery disease in health-care practice. Asian J Pharm Clin Res 2020;13:1-4.
8. Barrow K. Diabetes Symptoms, Diagnosis, Treatment of Diabetes. New York: Times Health Information; 2018.
9. Klein G, Kim J, Himmeldirk K, Cao Y, Chen X. Antidiabetic and anti-obesity activity of Lagerstroemia speciosa. Evid Based Complement Altern Med 2007;4:401-4.
10. American Diabetes Association. Microvascular complications and foot care: Standards of medical care in diabetes 2020. Diabetes Care 2020;43 Suppl 1:S135-51.
11. Patil MS, Gaikwad DK. A critical review on medicinally important oil yielding plant laxmitaru (Simarouba glauca DC.). J Pharm Sci Res 2011;3:1195-213.
12. Asha J, Elango K, Palur RA, SubbaRao VM. Therapeutic potential of phytochemicals isolated from Simarouba glauca for inhibiting cancers: A review. Syst Rev Pharm 2019;10:73-80.
13. Govindaraju K, Darukeshwara J, Srivastava AK. Studies on protein characteristics and toxic constituents of Simarouba glauca oilseed meal. Food Chem Toxicol 2009;47:1327-32.
14. Shantha TR, Prathapa RM, Rama RV, Kishore KR, Sulochana B. Pharmacognostical studies on the leaves of lakshmitaru (Simarouba Glauca Dc.), a well known anti-cancerous plant. World J Pharm Pharm Sci 2016;5:1717-27.
15. Edward FG, Dennis W. Simarouba glauca Paradise Tree. Geneva, Switzerland: Fact Sheet ST-590; 1994.
16. Iyyappan A, Minnady M, Rathinam P. Detail study on Simarouba glauca (DC) plant for its medicinal importance-a review. Int J Adv Sci Res Manag 2017;7:86-95.
17. Alam N, Sharma KR. Estimation of phenolic content, flavonoid content, antioxidant, and alpha-amylase inhibitory activity of some selected plants from Siraha district Nepal. Asian J Pharm Clin Res 2020;13:18-23.
18. Sneha KS, Aswathy V, Meera CR. Study on the phytochemical, antibacterial and antioxidant activities of Simarouba glauca. South Indian J Biol Sci 2016;2:119-24.
19. Khandelwal KR. Practical pharmacognosy techniques and experiments. Nirali Prakashan 2008;25:1-25.
20. Sehira BK, Hathrine L. General techniques involved in phytochemical analysis. Int J Pharm Res Chem Sci 2015;2:25-32.
21. Montefusco-Pereira CV, de Carvalho MJ, de Araújo Boleti AP, Teixeira LS, Matos HR, Lima ES. Antioxidant, anti-inflammatory, and hypoglycemic effects of the leaf extract from Passiflora Nitida Kunth.Appl Biochem Biotechnol 2013;170:1367-78.
22. Oyedepo OO, Famurewa AJ. Antiprotease and membrane stabilizing activities of extracts of fagra zanthoxiloides. Int J Pharmacogn 1995;33:65-9.
23. Sadique J, Al-Rqobah NA, Bughaith MF, El-Gindy AR. The bioactivity of certain plants on the stabilization of RBC membrane system. Fitoterapia 1989;60:525-35.
24. Narkhede MB, Ajimire PV, Wagh AE, Manoj M, Shivashanmugam AT. In vitro antidiabetic activity of Caesalpina digyna (R.) methanol root extract. Asian J Plant Sci 2011;1:101-6.
25. Megha GC, Bhoomi BJ, Kinnari NM. In vitro anti-diabetic and anti-inflammatory activity of stem bark of Bauhinia purpurea. Bull Pharm Med Sci 2013;1:139-50.
26. Gallagher AM, Flatt PR, Duffy G, Abdel-Wahab YH. The effects of traditional anti diabetic plants on in vitro glucose diffusion. Nutr Res 2003;23:413-24.
27. Edward CA Johnson IT, Read NW. Do viscous polysaccharides slow absorption by inhibiting diffusion or convection. Eur J Clin Nutr 1988;42:307-12
28. Vijayalakshmi K, Selvaraj CI, Sindhu S, Arumugam P. In vitro investigation of antidiabetic potential of selected traditional medicinal plants. Int J Pharmacogn Phytochem Res 2014;6:856-61.
29. Osagie-Eweka SD. Phytochemical analyses and comparative in-vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (paradise tree). Afr J Plant Sci 2018;12:7-16.
30. Bharat M, Mastewal A, Dessalegn AG. Evaluation of anti-ulcer activity of the leaf extract of Osyris quadripartite Decne. (Santalaceae) in rats. J Exp Pharmacol 2017;9:1-11.
31. Sakat SS, Tupe PN, Juvekar AR. In-vitro anti-inflammatory activity of aqueous and methanol extracts aqueous and methanol extracts of Erythrina indica leaves. Pharamacol Online 2009;3:221-9.
32. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol 1968;20:169-73.
33. Felix N, Jennifer M, Victoria AL, Derek S, Heather AR, Gordon J. Anti-proliferative and potential anti-diabetic effects of phenolic-rich extracts from edible marine algae. Food Chem 2011;126:1006-12.
34. Biresh KS, Ravi K, Reeta, Verma SC, Shashi P, Ramaiah M, et al. Evaluation of In vitro anti-inflammatory activity and HPTLC analysis of plant Phyllanthus fraternus. Int J Curr Pharm Res 2017;9:198-200.
35. Krishna RM, Tejaswini G. Anti-inflammatory activity of Erythrina variegata. Int J Pharm Pharm Sci 2015;7:386-8.
36. Sushma D, Randhir S. Antidiabetic activity of methanolic extract of Nepeta Hindostana herb in streptozotocin induced diabetes in rats. Int J Pharm Pharm Sci 2016;8:330-5.
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How to Cite
R, A., and B. MISHRA. “EVALUATION OF ANTI-INFLAMMATORY AND ANTIDIABETIC ACTIVITY OF SIMAROUBA GLAUCA BARK EXTRACT: AN IN VITRO STUDY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 8, May 2020, pp. 74-78, doi:10.22159/ajpcr.2020.v13i8.37916.
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