EFFECT OF STERCULIA QUADRIFIDA R. BR BARK TO IN VITRO DPPH RADICAL AND GLUTATHIONE PEROXIDASE ON DIABETIC WISTAR RATS

  • RAMBU KONDA A PRAING Pharmaceutical Biology Research Group, Faculty of Pharmacy, Setia Budi University, Indonesia.
  • TITIK SUNARNI Pharmaceutical Biology Research Group, Faculty of Pharmacy, Setia Budi University, Indonesia.

Abstract

Objective: The aims of the research were to evaluate the antioxidant effect of Sterculia quadrifida R.Br bark by in vitro 1,1-biphenyl-2-picryl-hydrazyl (DPPH) radical scavenging method and glutathione peroxidase (GPx) activity on diabetic Wistar rats model.


Methods: S. quadrifida bark powder was extracted in ethanol 70% by maceration method. The antioxidant activity of ethanol extract was tested by DPPH radical scavenging method. The activity of GPx was evaluated from the liver of alloxan-induced diabetic rats. The antioxidant activity was determined by measuring the absorbance using ultraviolet-visible spectrophotometry method.


Results: The ethanol extract of S. quadrifida bark had a strong DPPH radical scavenging activity, with the IC50 value was 4.86±0.01 ug/ml, besides that the extract also showed significantly activity of GPx (p<0.05) at a dose of 65, 130, and 260 mg/kg bw.


Conclusion: The ethanol extract of S. quadrifida bark had a potent antioxidant activity by DPPH radical scavenging and GPx activity.

Keywords: Sterculia quadrifida R.Br, 1,1-biphenyl-2-picryl-hydrazyl, Glutathione peroxidase, Antioxidant

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A PRAING, R. K., and T. SUNARNI. “EFFECT OF STERCULIA QUADRIFIDA R. BR BARK TO IN VITRO DPPH RADICAL AND GLUTATHIONE PEROXIDASE ON DIABETIC WISTAR RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 11, Sept. 2020, pp. 128-31, doi:10.22159/ajpcr.2020.v13i11.38805.
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