• PAULA ALEJANDRA GIRALDO VILLAMIL Department of Chemical Pharmaceutical Program, Universidad de Ciencias Aplicadas y Ambientales U.D.C.A., Bogotá Colombia.
  • ANDRÉS CAMILO ANDRADE BURBANO Department of Chemical Pharmaceutical Program, Universidad de Ciencias Aplicadas y Ambientales U.D.C.A., Bogotá Colombia.
  • LUIS POMBO OSPINA Centro de Investigacion, Fundación Universitaria Juan N. Corpas, Bogotá Colombia.
  • JANETH ARIAS PALACIOS Department of Microbiology, Industrial Microbiology Program, Industrial Environmental and Biotechnology Group, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • ÓSCAR EDUARDO RODRÍGUEZ AGUIRRE Environmental Engineering Program, Faculty of Engineering, Research Group CHOC-IZONE, Universidad El Bosque, Bogotá, Colombia.


Objective: The objective of the study was to determine the antimicrobial activity of leaf and flower extract in Chromolaena scabra (L. f.) R.M. King and H. Rob., against selected strains of bacteria and fungi.

Methods: The agar diffusion method with plate perforation was developed; the microorganisms used were strains of Staphylococcus aureus and Escherichia coli, Aspergillus niger, and Penicillium digitatum. Rifampicin was used as a positive control. The evaluation was performed by measuring the diameter of the growth inhibition zones around the holes. The inhibitory effect of the plant extracts was obtained by its efficiency compared to the positive control. A comparison with fluconazole and ketoconazole was performed to determine how much of the extract is required to cause inhibition of fungal growth from the standard.

Results: IC50 was determined by relating the ln of mass evaluated with respect to the square of the inhibition halo; ethanolic extracts of leaves and flowers of petroleum ether with IC50 values of 85.8 mg/ml and 50.3 mg/ml showed the highest inhibitory effect against S. aureus; the extract of petroleum ether and ethanol from leaves with IC50 of 64 mg/ml and 60 mg/ml, respectively. They were effective with A. niger. Leaf petroleum ether extract showed the best relative antifungal activity against A. niger with respect to fluconazole equivalent to 459.51 when fluconazole is 1.0.

Conclusion: The extracts with high potential to inhibit the growth of microorganisms were determined to be ether flowers of petroleum and ethanol leaf extracts.

Keywords: Agar diffusion method, Chromolaena scabra, antimicrobial activity, Soxhlet extraction, Staphylococcus aureus, Aspergillus niger, Penicillium digitatum

Author Biography

PAULA ALEJANDRA GIRALDO VILLAMIL, Department of Chemical Pharmaceutical Program, Universidad de Ciencias Aplicadas y Ambientales U.D.C.A., Bogotá Colombia.

Departamento de Microbiologia. Grupo de Biotecnologia Ambiental e Industrial


1. Arunkumar K, Chandrashekar KR. Phytochemical evaluation and in vitro antimicrobial and antioxidant studies of leaf and stem bark extracts of Polyalthia fragrans (Dalz.) Bedd. An endemic species of Western Ghats. International J Pharm Pharm Sci 2017;9:20-4.
2. Pandey S. Antibacterial and antifungal activities of Ocimum gratissimum L. Int J Pharm Pharm Sci 2017;9:26-31.
3. Pruthvi ML, Mahesh MK, Rohini SM. Efficacy of Euphorbia heterophylla Latex against pathogenic bacteria and fungi. Asian J Pharm Clin Res 2020;1:141-5.
4. Sekyere JO, Asante J. Emerging mechanisms of antimicrobial resistance in bacteria and fungi: Advances in the era of genomics. Future Microbiol 2018;13:241-62.
5. Amita SR, Shobha KL, Manjunath SH, Sreedhara PK. In vitro antibacterial and antifungal activities of aqueous and ethanolic leaf extracts of Acacia auriculiformis. Asian J Pharm Clin Res 2018;11:480-2.
6. Igboh MN, Ikewuchi CJ, Catherine C. Chemical profile of Chromolaena odorata L. (King and Robinson) leaves. Pak J Nutr 2009;8:521-4.
7. Taleb-Contini SH, Salvador MJ, Watanabe E, Ito IY, Oliveira CR. Antimicrobial activity of flavonoids and steroids isolated from two Chromolaena Species. Rev Bras Ciênc Farma 2003;39:403-8.
8. Díaz-Piedrahita S, Rodríguez-Cabeza BV. Novedades en asteráceas colombianas-I. Rev Acad Colomb Cienc 2011;35:411-24.
9. Cole MD. Key antifungal, antibacterial and anti-insect assays-a critical rewiew. Biochem Syst Ecol Oxf 1994;22:837-56.
10. Grove DC, Randall WA. Assay Methods of Antibiotics: A Laboratory Manual [Antibiotics Monographs]. New York: Medical Encyclopedia Inc.; 1955. p. 80.
11. Narváez M, Rodríguez OE, Arias J. Antimicrobial activity of Hymenaea courbaril L. Fruit. Asian J Pharm Clin Res 2020;13:200-3.
12. Cuenca M, Medallo E, Rodríguez JL. Importancia clínica de los mecanismos de resistencia de los hongos filamentosos a los antifúngicos. Unidad Micol 2002;20:523-30.
13. Adewumi AM, Olusola-Makinde O, Kolawole OM. Evaluation of phytochemical constituents and antibacterial activity of Chromolaena odorata L. Leaf extract against selected multidrug resistant bacteria isolated from wounds. South Asian J Res Microbiol 2020;5:1-9.
14. Catalán M, Montejo JC. Antifúngicos sistémicos. Farmacodinamia y farmacocinètica. Rev Iberoam Micol 2006;23:39-49.
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How to Cite
GIRALDO VILLAMIL, P. A., A. C. ANDRADE BURBANO, L. P. OSPINA, J. A. PALACIOS, and ÓSCAR E. RODRÍGUEZ AGUIRRE. “DETERMINATION OF ANTIMICROBIAL ACTIVITY IN LEAVES AND FLOWERS OF CHROMOLAENA SCABRA (L. F.) R.M. KING AND H. ROB.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 9, Aug. 2020, pp. 53-56, doi:10.22159/ajpcr.2020.v13i9.38835.
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