• VIDYACHARANI DHA Department of Botany, PSG College of Arts & Science, Coimbatore, Tamil Nadu, India.
  • ARUNPRASATH A Department of Botany, PSG College of Arts & Science, Coimbatore, Tamil Nadu, India.


Objectives: The present work is to evaluate the presence of phenol and flavonoid contents, 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) activity and antimicrobial activity of Corbichonia decumbens leaf extracts.

Methods: C. decumbens leaves were collected, shaded, dried, powdered, and were subjected to Soxhlet apparatus for extraction using ethanol and petroleum ether. The ethanol and petroleum ether extract of the plant was tested for phenol, flavonoid, H2O2 contents, and DPPH scavenging activity using standard procedures. It is also subjected to antimicrobial activity against pathogenic bacteria and fungus using the well diffusion method.

Results: The result revealed that the leaf extracts contain phenol, flavonoid, and H2O2 contents. The values of DPPH radical scavenging activity recorded for ethanol and petroleum ether extract were 55.90±0.10 and 41.23±0.20, respectively. Antimicrobial activities of ethanolic leaf extracts showed lesser values compared to standard values.

Conclusion: The result of this study showed the presence of phenol and flavonoid content, evidenced the potential antioxidant and antimicrobial activity against all tested micro-organisms.

Keywords: Corbichonia, Phenol, 2-Diphenyl-1-picrylhydrazyl, Ethanol and Antimicrobial activity

Author Biography

ARUNPRASATH A, Department of Botany, PSG College of Arts & Science, Coimbatore, Tamil Nadu, India.

Doing M.Sc Botany in PG and Research Department of Botany, PSG College of Arts & Science, Coimbatore,

Tamil Nadu,  India – 641 014.


1. Singh SK. Antibacterial activity of different extract of Moringa oleifera leaf against some pathogenic bacteria. J Pharm Sci Innov 2013;2:13-5.
2. Thirumurugan K. Antimicrobial activity and phytochemical analysis of selected Indian folk medicinal plants. Steroids 2010;1:430-4.
3. Geissman TA. Flavonoid compounds, tannins, lignins and, related compounds. Compr Biochem 1963;9:213-50.
4. Inayatullah S, Prenzler P, Obied H, Rehman A, Mirza B. Bioprospecting traditional Pakistani medicinal plants for potent antioxidants. Food Chem 2012;132:222-9.
5. Çali?kan O, Polat AA. Phytochemical and antioxidant properties of selected fig (Ficus carica L.) accessions from the eastern Mediterranean region of Turkey. Sci Hortic 2011;128:473-8.
6. Duarte MC, Figueira GM, Sartoratto A, Rehder VL, Delarmelina C. Anti-Candida activity of Brazilian medicinal plants. J Ethnopharmacol 2005;97:305-11.
7. Mahesh B, Satish S. Antimicrobial activity of some important medicinal plant against plant and human pathogens. World J Agric Res 2008;4:839-43.
8. Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 1998;46:4113-7.
9. Bhalodia NR, Acharya RN, Shukla VJ. Evaluation of in vitro antioxidant activity of hydroalcoholic seed extratcs of Cassia fistula linn. Free Radic Res 2011;1:68-76.
10. Patel NB, Patel JC. Synthesis and antimicrobial activity of 3-(1, 3, 4-oxadiazol-2-yl) quinazolin-4 (3H)-ones. Sci Pharm 2010;78:171-94.
11. Taylor RS, Edel F, Manandhar NP, Towers GH. Antimicrobial activities of southern Nepalese medicinal plants. J Ethnopharmacol 1996;50:97-102.
12. Kumar AR, Sudha TS. In vitro antioxidant and antibacterial activity of aqueous and methanolic extract of Mollugo nudicaulis Lam. leaves. Asian Pac J Trop Biomed 2012;2:S895-900.
13. Deng Y, Zhao Y, Padilla-Zakour O, Yang G. Polyphenols, antioxidant and antimicrobial activities of leaf and bark extracts of Solidago canadensis L. Ind Crops Prod 2015;74:803-9.
14. Martin-Puzon JJ, Rivera WL. Free-radical scavenging activity and bioactive secondary metabolites from various extracts of Glinus oppositifolius (L.) Aug. DC. (Molluginaceae) roots, stems and leaves. Asian Pac J Trop Biomed 2015;5:711-5.
15. Tunasamy K, Suryadevara N, Athimoolam T. Screening of Vernonia amygdalina leaf extracts for antioxidant and antimicrobial activity. Mater Today Proc 2019;16:1809-18.
16. Keser S, Celik S, Turkoglu S, Yilmaz O, Turkoglu I. Hydrogen peroxide radical scavenging and total antioxidant activity of hawthorn. Chem J 2012;2:9-12.
17. Atere TG, Akinloye OA, Ugbaja RN, Ojo DA, Dealtry G. In vitro antioxidant capacity and free radical scavenging evaluation of standardized extract of Costus afer leaf. Food Sci Hum Wellness 2018;7:266-72.
18. Ozsoy N, Can A, Yanardag R, Akev N. Antioxidant activity of Smilax excelsa L. leaf extracts. Food Chem 2008;110:571-83.
19. Godwill EA, Unaegbu M, Esther AU, Gloria OA, Kingsley AN, Aiyegoro OA, et al. Antioxidant and antidiabetic activities of the seed and leaf extracts of Chrysophyllum albidum. Asian Pac J Trop Biomed 2016;6:642-9.
20. Rahman MA, Bin IT, Islam S. Antioxidative, antimicrobial and cytotoxic effects of the phenolics of Leea indica leaf extract. Saudi J Biol Sci 2013;20:213-25.
21. Choudhary K, Mathur N, Chaudhary A, Chaudhary BL. Assessment of the antimicrobial potency of leaf extracts from Vitex nugundo and Gloriosa superba. Pharmacogn Rev 2011;3:80-4.
22. Naves VM, dos Santos MH, Ribeiro IS, da Silva CA, Silva NC, da Silva MA, et al. Antimicrobial and antioxidant activity of Garcinia brasiliensis extracts. S Afr J Bot 2019;124:244-50.
23. Pa R, Mathew L. Antimicrobial activity of leaf extracts of Justicia adhatoda L. in comparison with vasicine. Asian Pac J Trop Biomed 2012;2:S1556-60.
11 Views | 27 Downloads
How to Cite
DHA, V., and A. A. “EVALUATIONS OF SECONDARY METABOLITES ANTIOXIDANT AND ANTIMICROBIAL POTENTIAL IN LEAF EXTRACTS OF CORBICHONIA DECUMBENS (FORSSK.) EXELL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 11, Nov. 2020, pp. 181-3, doi:10.22159/ajpcr.2020.v13i11.39004.
Original Article(s)