EVALUATION OF THE FREE RADICAL AND ANTIOXIDANT ABILITIES IN ETHANOLIC EXTRACT OF THE Breynia patens (Roxb.) Benth. & Hook. f.
The present study evaluates the free radical and antioxidant abilities in the extract of Breynia patens. Some in vitro antioxidant assays were employed, Such as DPPH, ABTS, Reducing power assay, Ferrous ion chelating activity and Hydroxyl radical scavenging assays. The plant extract shows excellent activity in DPPH (IC50=43.47) and ABTS (6314.6Â±15.5 Âµmol/g). Reducing power assay indicates an increase in reductive ability, Ferrous ion chelating activity (IC50 = 303.03) and Hydroxyl radical scavenging assays (IC50=581.39). The results could be considered the plant as natural antioxidants and may be useful for curing diseases arising from oxidative deterioration and considered as a source of natural therapeutic agent.
Ali L, Alsanius BW, Rosberg AK, Svensson B, Nielsen T, Olsson ME. Effects of nutrition strategy on the levels of nutrients and bioactive compounds in blackber- ries. Eur Food Res Technol 2012;234:33-44.
Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci U S A 1993;90(17):7915-22.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-200.
Duthie GG, Duthie SJ, Kyle JA. Plant polyphenols in cancer and heart disease: implications as nutritional antioxidants. Nutr Res Rev 2000;13(1):79-106.
Enujiugha VN. The antioxidant and free radical-scavenging capacity of phenolics from African locust bean seeds (Parkia biglobosa). Adv Food Sci 2010;32(2):88-93.
Ferreira CF, Baptista P, Vilas-Boas M. Barros L. Free-radical scavenging capacity and reducing power of wild edible mushrooms from North East Portugal: Individual cap and stipe activity. Food Chem 2007;100:1511-6.
Gawron-Gzella A, Dudek-Makuch M, MatÅ‚awska I. DPPH radical scavenging activity and phenolic compound content in different leaf extracts from selected blackberry species. Acta Biol Cracov Ser Bot 2012;54(2):32-8.
GÃ¼lÃ§in I, Alici HA, Cesur M. Determination of in vitro antioxidant and radical scavenging activities of propofol. Chem Pharm Bull (Tokyo) 2005;53(3):281-5.
Hsu CL, Chen W, Weng YM, Tseng CY. Chemical composition,AQ3physical properties and antioxidant activities of yam flours as affected by different drying methods. Food Chem 2003;83:85-92.
Karakaya S, Kavas A. Antimutagenic activities of some foods. J Food Sci Agric 1999;79:237-42.
Klein SM, Cohen G, Cederbaum AI. Production of formaldehyde during metabolism of dimethyl sulfoxide by hydroxyl radical generating systems. Biochemistry 1981;20:6006-12.
Koksal E, Bursal E, Dikici E, Tozoglu F, Gulcin I. Antioxidant activity of Melissa officinalis leaves. J Med Plant Res 2011;5:217-22.
Kunchandy E, Rao MN. Oxygen radical scavenging activity of coumarin. Int J Pharmacogn 1990;58:237-40.
Lim YY, Lim TT, Tee JJ. Antioxidant properties of several tropical fruits: A comparative study. Food Chem 2007;103:1003-8.
Ozgen M, Reese RN, Tulio AZ Jr, Scheerens JC, Miller AR. Modified 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (abts) method to measure antioxidant capacity of Selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2â€™-diphenyl-1-picrylhydrazyl (DPPH) methods. J Agric Food Chem 2006;54(4):1151-7.
Porwal P, Shukla K, Mishra DK, Mahajan SC, Akhilesh T. Comparative in vitro antioxidant activty of Pongamia pinnata linn. leaves extracts and isolated compound. Int J Pharm Biol Arch 2010;1(1):69-75.
Rao MS, Raman MV. ???. Biochem Syst Ecol 2004;32:447-8.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999;26(9-10):1231-7.
Burdon RH. Free Radical Damage and its Control. Netherlands: Elsevier Science B.V; 1994. p. 125.
Singh N, Rajini PS. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 2004;85:611-6.
Sreejayan N, Rao NM. Free radical scavenging activity of Curcuminoids. Drug Res 1996;4:61-9.
Subhasree N, Shivakumar S, Sandhiya S, Agrawal A, Dubey GP. In vitro Assessment of antioxidant and antibacterial activities of salacia species - A comparative study. Int J Pharm Pharm Sci 2013;5:279-82.
Ting KN, Othman M, Telford G, Clarke G, Bradshaw TD, Khoo TJ, et al. Antioxidant cytoprotective growth inhibitory and immunomodulatory activities of extracts of Dysoxylum cauliflorum hiern, a Malaysian meliaceae. J Med Plants Res 2011;5:5867-72.
Yildirim A, Mavi A, Kara AA. Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem 2001;49(8):4083-9.
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