• PANKAJ SHARMA Department of Pharmacy, Institute of Pharmacy and Research, S.J.J.T University, Jhunjhunu, Rajasthan, India.



Acacia catechu, MCF-7, A431, HepG2, Polyphenols, Katha


Objective: The present study delves into the investigation of quantitative phytochemical in Katha powder, and it is in vitro cytotoxic activity.

Methods: Coarsely dried chips of Acacia catechu heartwood were treated with a 10% hydro-alcoholic solution to obtain Katha as the final product. The powdered Katha was standardized through pharmacognostic parameters. Phytochemical investigations were carried out to screen polyphenols (tannins and flavonoids) of interest which later were confirmed by thin-layer chromatography. The cytotoxicity effect of Katha powder on MCF-7, A431, and HepG2 cells was characterized by the trypan blue dye exclusion and MTT colorimetric assays technique. Control assay was carried out for samples containing only the appropriate volumes of blank solutions and showed no effect on cell growth. Different cells were exposed to Katha powder for about 48 h and performed cytotoxicity assays. The effect of Katha powder against these cell lines concentration range 10–100 μg/ml showed a decrease in percent cell viability in a dose-dependent manner, as compared with that of the control when examined by the trypan blue exclusion assay technique and MTT colorimetric assays technique.

Results: Quantitative phytochemical investigations were showed that Katha is rich in the content of polyphenols (tannins and flavonoids) and having good pharmacological potential. The effect of Katha powder against these cell lines concentration range 10–100 μg/ml showed a decrease in percent cell viability in a dose-dependent manner.

Conclusion: So from this investigation it is to be suggested that the Katha powder is rich in the phenolic compound and shows a good anticancer effect against MCF-7, A431, and HepG2 cells.


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Sharma P. Investigation on photodecomposition of standardised ethyl acetate fraction of Katha. Pharmacogn J 2020;12:815-20.

Chauhan NS. Medicinal and Aromatic Plants of Himachal Pradesh. New Delhi: Indus Publishing; 1999.

Meena AK, Bansal P, Kumar S. Plants-herbal wealth as a potential source of ayurvedic drugs. Asian J Tradit Med 2009;4:152-70.

Azab A, Nassar A, Azab AN. Anti-inflammatory activity of natural products. Molecules 2016;21:1321.

Zahin M, Aqil F, Ahmad I. The in vitro antioxidant activity and total phenolic content of four Indian medicinal plants. Int J Pharm Pharm Sci 2009;1:88-95.

Thakur AV, Ambwani S, Ambwani TK. Preliminary phytochemical screening and GC-MS analysis of leaf extract of Acacia catechu (Lf) Willd. Int J Herb Med 2018;6:81-5.

Hazra B, Sarkar R, Ghate NB, Chaudhuri D, Mandal N. Study of the protective effects of Katha (heartwood extract of Acacia catechu) in liver damage induced by iron overload. J Environ Pathol Toxicol Oncol 2013;32:229-40.

Lakshmi T, Geetha RV, Anitha R. Acacia catechu willd: A pharmacological review. Int J Curr Res Rev 2011;3:101-11.

Wang L, Shen X, Mi L, Jing J, Gai S, Liu, et al. Simultaneous determinations of four major bioactive components in Acacia catechu (Lf) Willd and Scutellaria baicalensis Georgi extracts by LC-MS/MS: Application to its herb-herb interactions based on pharmacokinetic, tissue distribution and excretion studies in rats. Phytomedicine 2019;56:64-73.

Srinivasan N. Pharmacognostical and phytochemical evaluation of Cassia alata Linn. J Med Plants 2018;6:69-77.

Maurya S, Singh D. Quantitative analysis of total phenolic content in Adhatoda vasica Nees extracts. Int J PharmTech Res 2010;2:2403-6.

Muthukumarasamy R, Ilyana A, Fithriyaani NA, Najihah NA, Asyiqin N, Sekar M. Formulation and evaluation of natural antioxidant cream comprising methanolic peel extract of Dimocarpus longan. Int J Pharm Clin Res 2016;8:1305-9.

Ghate NB, Chaudhuri D, Mandal N. In vitro assessment of Tinospora cordifolia stem for its antioxidant, free radical scavenging, and DNA protective potentials. Int J Pharm Bio Sci 2013;4:373-88.

Kalita P, Tapan BK, Pal TK, Kalita R. Estimation of total flavonoids content (TFC) and antioxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn. J Drug Deliv Ther 2013;3:33-7.

Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complement Alternat Med 2008;8:1-10.

Prakash NK, Ranjith M, Sripriya N, Lakshmi RP, Deepa S, Bhuvaneswari S. Antioxidant, free radical scavenging activity and GC-MS studies on Pedilanthus tithymaloides (l.) Poit. Int J Pharm Pharm Sci 2014;6:284-87.

John B, Sulaiman CT, George S, Reddy VR. Total phenolics and flavonoids in selected medicinal plants from Kerala. Int J Pharm Pharm Sci 2014;6:406-8.

Bhattacharya E, Dutta R, Chakraborty S, Biswas SM. Phytochemical profiling of Artocarpus lakoocha Roxb. Leaf methanol extract and its antioxidant, antimicrobial, and anti-oxidative activities. Asian Pac J Trop Biomed 2019;9:484-92.

Brownell LA, Chu M, Hong MF, Eu-Jin HY, Jia Q, Ping JI, et al. Compositions and Methods for Managing or Improving Bone Disorders, Cartilage Disorders, or Both. United States: The United States Patent Application US 15/663,793; 2018.

Khonkarn R, Okonogi S, Ampasavate C, Anuchapreeda S. Investigation of fruit peel extract as sources for compounds with antioxidant and antiproliferative activities against human cell lines. Food Chem Toxicol 2010;48:2122-9.

Chen IS, Quan SG, Golde DW. Human T-cell leukemia virus Type II transforms normal human lymphocytes. Proc Natl Acad Sci 1983;80:7006-9.

Dekosky BJ, Ippolito GC, Deschner RP, Lavinder JJ, Wine Y, Rawlings M, et al. High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire. Nat Biotechnol 2013;31:166-9.

Choudhari MK, Haghniaz R, Rajwade JM, Paknikar KM. Anticancer activity of Indian stingless bee propolis: An in vitro study. Evid Based Complement Alternat Med 2013;2013:1-10.

Strober W. Trypan blue exclusion test of cell viability. Curr Protoc Immunol 1997;21:3B.

Sutradhar BC, Park J, Hong G, Choi SH, Kim G. Effects of trypsinization on the viability of equine chondrocytes in cell culture. Pak Vet J 2010;30:232-8.

Tokunaga M, Kitamura K, Saito K, Iwane AH, Yanagida T. Single-molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy. Biochem Biophys Res Commun 1997;235:47-53.

Kroll A, Pillukat MH, Hahn D, Schnekenburger J. Current in vitro methods in nanoparticle risk assessment: Limitations and challenges. Eur J Pharm Biopharm 2009;72:370-7.

Monga J, Pandit S, Chauhan CS, Sharma M. Cytotoxicity and apoptosis induction in human breast adenocarcinoma MCF-7 cells by (+)-cyanidan-3-ol. Exp Toxicol Pathol 2013;65:1091-100.

Murphy M, Drago J, Bartlett PF. The fibroblast growth factor stimulates the proliferation and differentiation of neural precursor cells in vitro. J Neurosci Res 1990;25:463-75.

Mpoke SS, Wolfe J. Differential staining of apoptotic nuclei in living cells: Application to macronuclear elimination in Tetrahymena. J Histochem Cytochem 1997;45:675-83.

Allen RT, Hunter WJ, Agrawal DK. Morphological and biochemical characterization and analysis of apoptosis. J Pharmacol Toxicol Methods 1997;37:215-28.

Panchal PK, Meena SK, Singh K, Sharma N. Anticancer and antimicrobial potential of barleria prionitis leaves ethanol extract. Int J Pharm Pharm Sci 2018;10:100-3.

Sharma M, Agrawal SK, Sharma PR, Chadha BS, Khosla MK, Saxena AK. Cytotoxic and apoptotic activity of essential oil from Ocimum viride towards Colo 205 cells. Food Chem Toxicol 2010;48:336-44.



How to Cite

SHARMA, P. “INVESTIGATION ON PHARMACOGNOSY OF KATHA POWDER AS WELL AS IT’S IN VITRO CYTOTOXIC ACTIVITY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 14, no. 1, Jan. 2021, pp. 133-40, doi:10.22159/ajpcr.2021.v14i1.39717.



Original Article(s)