investigation Investigation on Pharmacognosy of Katha powder as well as it’s in- vitro cytotoxic activity

Investigation on Pharmacognosy of Katha powder as well as it’s in- vitro cytotoxic activity

  • pankaj sharma s.j.j.t university

Abstract

ABSTRACT


Objective: The present study delves for investigation of pharmacognosy, quantitative phytochemical in Katha powder and manifest it’s in vitro cytotoxic activity. Methods: Coarsely dried chips of Acacia catechu heart wood was treated with 10 % hydro-alcoholic solution to obtain Katha as final product. The powdered Katha was standardised via pharmacognostic parameters. Phytochemical investigations were carried out to screen polyphenols (tannins & flavonoids) of interest which later were confirmed by thin layer chromatography (TLC). The cytotoxicity effect of Katha powder on MCF-7, A431 and HepG2 cells were characterized by the tryphan blue dye exclusion and MTT colorimetric assays technique. Control assay were carried out for samples containing only the appropriate volumes of blank solutions and showed no effect on the cell growth. Different cell were exposed to Katha powder for about 48 hours and performed cytotoxicity assays. The effect of Katha powder against these cell lines (concentration range 10-100 µg/ml showed a decrease in percent cell viability in a dose- dependent manner, as compared with that of the   control when examined by the tryphan blue exclusion assay technique and MTT colorimetric assays technique. Results: Quantitative phytochemical investigations were showed that Katha is rich content of polyphenols (tannins & flavonoids) and having good pharmacological potential. The effect of Katha powder against these cell lines (concentration range 10-100 µg/ml showed a decrease in percent cell viability in a dose- dependent manner.


Conclusion: So from this investigation it is to be suggested that the Katha powder is rich of phenolic compound and show good anticancer effect against MCF-7, A431 and HepG2 cells.

Keywords: Acacia catechu, MCF-7, A431, HepG2, polyphenols, Katha.

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sharma, pankaj. “Investigation Investigation on Pharmacognosy of Katha Powder As Well As it’s In- Vitro Cytotoxic Activity: Investigation on Pharmacognosy of Katha Powder As Well As it’s In- Vitro Cytotoxic Activity”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 1, Jan. 2021, pp. 133-40, https://innovareacademics.in/journals/index.php/ajpcr/article/view/39717.
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