investigation Investigation on Pharmacognosy of Katha powder as well as it’s in- vitro cytotoxic activity

Investigation on Pharmacognosy of Katha powder as well as it’s in- vitro cytotoxic activity

  • pankaj sharma s.j.j.t university



Objective: The present study delves for investigation of pharmacognosy, quantitative phytochemical in Katha powder and manifest it’s in vitro cytotoxic activity. Methods: Coarsely dried chips of Acacia catechu heart wood was treated with 10 % hydro-alcoholic solution to obtain Katha as final product. The powdered Katha was standardised via pharmacognostic parameters. Phytochemical investigations were carried out to screen polyphenols (tannins & flavonoids) of interest which later were confirmed by thin layer chromatography (TLC). The cytotoxicity effect of Katha powder on MCF-7, A431 and HepG2 cells were characterized by the tryphan blue dye exclusion and MTT colorimetric assays technique. Control assay were carried out for samples containing only the appropriate volumes of blank solutions and showed no effect on the cell growth. Different cell were exposed to Katha powder for about 48 hours and performed cytotoxicity assays. The effect of Katha powder against these cell lines (concentration range 10-100 µg/ml showed a decrease in percent cell viability in a dose- dependent manner, as compared with that of the   control when examined by the tryphan blue exclusion assay technique and MTT colorimetric assays technique. Results: Quantitative phytochemical investigations were showed that Katha is rich content of polyphenols (tannins & flavonoids) and having good pharmacological potential. The effect of Katha powder against these cell lines (concentration range 10-100 µg/ml showed a decrease in percent cell viability in a dose- dependent manner.

Conclusion: So from this investigation it is to be suggested that the Katha powder is rich of phenolic compound and show good anticancer effect against MCF-7, A431 and HepG2 cells.

Keywords: Acacia catechu, MCF-7, A431, HepG2, polyphenols, Katha.


1. Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology. 2014; 4: 1777.
2. Sharma P. Investigation on Photodecomposition of Standardised Ethyl Acetate Fraction of Katha. Pharmacogn J. 2020; 12(4):815-20.
3. Chauhan NS. Medicinal and aromatic plants of Himachal Pradesh. Indus publishing; 1999.
4. Meena AK, Bansal P, Kumar S. Plants-herbal wealth as a potential source of ayurvedic drugs. Asian Journal of Traditional Medicines. 2009; 4(4):152-70.
5. Azab A, Nassar A, Azab AN. Anti-inflammatory activity of natural products. Molecules. 2016; 21(10):1321.
6. Mehta A. 11 Pharmacology of Medicinal Plants with Antioxidant Activity. Plants as a Source of Natural Antioxidants. 2014; 18:225.
7. Thakur AV, Ambwani S, Ambwani TK. Preliminary phytochemical screening and GC-MS analysis of leaf extract of Acacia catechu (Lf) Willd. International Journal of Herbal Medicine. 2018; 6(2):81-5.
8. Hazra B, Sarkar R, Ghate NB, Chaudhuri D, Mandal N. Study of the protective effects of Katha (heartwood extract of Acacia catechu) in liver damage induced by iron overload. Journal of Environmental Pathology, Toxicology and Oncology. 2013; 32 (3).
9. Lakshmi T, Geetha RV, Anitha R. Acacia catechu Willd: A Pharmacological review. International Journal of Current Research and Review. 2011; 3 (5):101-11.
10. Wang L, Shen X, Mi L, Jing J, Gai S, Liu X, Wang Q, Zhang S. Simultaneous determinations of four major bioactive components in Acacia catechu (Lf) Willd and Scutellaria baicalensis Georgi extracts by LC–MS/MS: Application to its herb–herb interactions based on pharmacokinetic, tissue distribution and excretion studies in rats. Phytomedicine. 2019; 56:64-73.
11. Srinivasan N. Pharmacognostical and phytochemical evaluation of Cassia alata Linn. Journal of Medicinal Plants. 2018; 6(5):69-77.
12. Maurya S, Singh D. Quantitative analysis of total phenolic content in Adhatoda vasica Nees extracts. International Journal of Pharm Tech Research. 2010; 2(4):2403-6.
13. Muthukumarasamy R, Ilyana A, Fithriyaani NA, Najihah NA, Asyiqin N, Sekar M. Formulation and evaluation of natural antioxidant cream comprising methanolic peel extract of Dimocarpus longan. International Journal of Pharmaceutical and Clinical Research. 2016; 8(9):1305-9.
14. Ghate NB, Chaudhuri D, Mandal N. In vitro assessment of Tinospora cordifolia stems for its antioxidant, free radical scavenging and DNA protective potentials. Int J Pharm Bio Sci. 2013; 4:373-88.
15. Kalita P, Tapan BK, Pal TK, Kalita R. Estimation of total flavonoids content (TFC) and anti oxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn. Journal of Drug delivery and Therapeutics. 2013; 3(4):33-7.
16. Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC complementary and Alternative Medicine. 2008; 8(1):1-0.
17. Mehta V, Sharma A, Kailkhura PA, Malairaman UD. Antioxidant, anti-inflammatory, and antidiabetic activity of hydroalcoholic extract of Ocimum sanctum: an in-vitro and in-silico study. 2016; 9(5): 44-49.
18. Blainski A, Lopes GC, De Mello JC. Application and analysis of the folin ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L. Molecules. 2013; 18(6):6852-65.
19. Bhattacharya E, Dutta R, Chakraborty S, Biswas SM. Phytochemical profiling of Artocarpus lakoocha Roxb. leaf methanol extract and its antioxidant, antimicrobial and anti-oxidative activities. Asian Pacific Journal of Tropical Biomedicine. 2019; 9(11):484.
20. Brownell LA, Chu M, Hong MF, Eu-Jin HY, Jia Q, Ping JI, Kim HJ, Kim MR, Kim TW, Lee YC, Nam JB, inventors. Compositions and Methods for Managing or Improving Bone Disorders, Cartilage Disorders, or Both. United States patent application US 15/663,793. 2018.
21. Khonkarn R, Okonogi S, Ampasavate C, Anuchapreeda S. Investigation of fruit peel extracts as sources for compounds with antioxidant and antiproliferative activities against human cell lines. Food and Chemical Toxicology. 2010; 48(8-9):2122-9.
22. Chen IS, Quan SG, Golde DW. Human T-cell leukemia virus type II transforms normal human lymphocytes. Proceedings of the National Academy of Sciences. 1983; 80(22):7006-9.
23. Dekosky BJ, Ippolito GC, Deschner RP, Lavinder JJ, Wine Y, Rawlings BM et al., High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire. Nature biotechnology. 2013; 31(2):166-9.
24. Choudhari MK, Haghniaz R, Rajwade JM, Paknikar KM. Anticancer activity of Indian stingless bee propolis: an in vitro study. Evidence-Based Complementary and Alternative Medicine. 2013; 1-10.
25. Strober W. Trypan blue exclusion test of cell viability. Current protocols in immunology. 1997; 21 (1): A-3B.
26. Sutradhar BC, Park J, Hong G, Choi SH, Kim G. Effects of trypsinization on viability of equine chondrocytes in cell culture. Pak. Vet. J. 2010; 30:232-8.
27. Tokunaga M, Kitamura K, Saito K, Iwane AH, Yanagida T. Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy. Biochemical and biophysical research communications. 1997; 235(1):47-53.
28. Kroll A, Pillukat MH, Hahn D, Schnekenburger J. Current in vitro methods in nanoparticle risk assessment: limitations and challenges. European journal of Pharmaceutics and Biopharmaceutics. 2009; 72 (2):370-7.
29. Monga J, Pandit S, Chauhan CS, Sharma M. Cytotoxicity and apoptosis induction in human breast adenocarcinoma MCF-7 cells by (+)-cyanidan-3-ol. Experimental and Toxicologic Pathology. 2013; 65(7-8):1091-100.
30. Murphy M, Drago J, Bartlett PF. Fibroblast growth factor stimulates the proliferation and differentiation of neural precursor cells in vitro. Journal of neuroscience research. 1990; 25(4):463-75.
31. Mpoke SS, Wolfe J. Differential staining of apoptotic nuclei in living cells: application to macronuclear elimination in Tetrahymena. Journal of Histochemistry & Cytochemistry. 1997; 45 (5):675-83.
32. Allen RT, Hunter III WJ, Agrawal DK. Morphological and biochemical characterization and analysis of apoptosis. Journal of pharmacological and toxicological methods. 1997; 37 (4):215-28.
33. Liu K, Liu PC, Liu R, Wu X. Dual AO/EB staining to detect apoptosis in osteosarcoma cells compared with flow cytometry. Medical science monitor basic research. 2015; 21:15.
34. Sharma M, Agrawal SK, Sharma PR, Chadha BS, Khosla MK, Saxena AK. Cytotoxic and apoptotic activity of essential oil from Ocimum viride towards COLO 205 cells. Food and Chemical Toxicology. 2010; 48 (1):336-44.
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sharma, pankaj. “Investigation Investigation on Pharmacognosy of Katha Powder As Well As it’s In- Vitro Cytotoxic Activity: Investigation on Pharmacognosy of Katha Powder As Well As it’s In- Vitro Cytotoxic Activity”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 1, Jan. 2021, pp. 133-40,
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