INVESTIGATION ON PHARMACOGNOSY AS WELL AS THE ANTIOXIDANT, ANTI-INFLAMMATORY POTENTIAL OF THE KATHA POWDER

  • PANKAJ SHARMA Department of Pharmacy, Government Pharmacy College Seraj, Mandi, Himachal Pradesh, India.
  • RAJU L Department of Pharmacy, Government Pharmacy College Seraj, Mandi, Himachal Pradesh, India.

Abstract

Objective: The objective of the study was to investigate the pharmacognosy as well as the antioxidant, anti-inflammatory potential of the Katha powder.


Methods: The Coarsely dried chips of Acacia catechu heartwood were treated with 10 % hydro-alcoholic solution to obtain Katha as the final product. The powdered Katha was standardized through pharmacognostic parameters. This Katha power is showing the good solubility in the hot water having astringent in the taste. The powder microscopy of the Katha powder is to be demonstrated fragments of acicular crystals, fibers, and bordered pitted vessels. Katha powder antioxidant potential is to be accessed by using the 2, 2-diphenyl-1-picryl hydrazyl assay and NO Scavenging assay using ascorbic acid as a standard drug. Further, the Katha powder is to be subjected for the assessment of its anti-inflammatory potential by the use of heat-induced hemolysis as well as hypotonicity-induced hemolysis approach by the use of the aspirin or diclofenac sodium as a standard drug.


Results: Microscopical investigations were showed that Katha showing the presence of fragments of acicular crystals, fibers, and bordered pitted vessels. In vitro study shows that the Katha powder has excellent antioxidant as well as anti-inflammatory potential in a dose-dependent manner in comparison of the result of heartwood of A. catechu.


Conclusion: So from this investigation, it is to be suggested that the Katha powder is rich in the phenolic compound and the experimentation study shows that the drug is to possess a good antioxidant as well as anti-inflammatory property.

Keywords: Acacia catechu, Polyphenolics, Katha, 2, 2-diphenyl-1-picryl hydrazyl, Nitric oxide

References

1. Hazra B, Sarkar R, Biswas S, Mandal N. The antioxidant, iron chelating and DNA protective properties of 70% methanolic extract of Katha (Heartwood extract of Acacia catechu). J Complement Integr Med 2010;7:1-20.
2. Sharma, P. Investigation on pharmacognosy of Katha powder as well as its in vitro cytotoxic activity. Asian J Pharm Clin Res 2021;14:133-40.
3. Zahin M, Aqil F, Ahmad I. The in vitro antioxidant activity and total phenolic content of four Indian medicinal plants. Int J Pharm Pharm Sci 2009;1: 88-95.
4. Mokhtar MA, Ahmad MA, Nizami Q, Ahmad FJ, Rahman MM. The wound-healing effect of an Unani formulation Marham-e-Ral in albino rats. Int J Adv Pharm Med Bioallied Sci 2014;2:20-4.
5. Jayasekhar P, Mohanan PV, Rathinam K. Hepatoprotective activity of ethyl acetate extract of Acacia catechu. Indian J Pharmacol 1997;29:426-8.
6. Sharma P. Investigation on photodecomposition of standardised ethyl acetate fraction of Katha. Pharmacogn J 2020;12:815-20.
7. Karnik MG, Sharma OP, Dobhal NP. Pilot plant production of Katha and cutch from Khair tree Acacia catechu Willd. Indian Forester 1965;91:778-80.
8. Singh KN, Lal, B. Notes on traditional uses of Khair (Acacia catechu Willd.) by inhabitants of Shivalik range in Western Himalaya. Ethnobot Leaf 2006;10:109-12.
9. Jain RA, Patni VI, Arora DK. Isolation and identification of flavonoid “quercetin” from Acacia catechu (Lf) Willd-A Katha yielding plant. J Phytol Res 2007;20:43-5.
10. Sharma, RP. Yield tables of Acacia catechu (Khair) (plantation origin) for various levels of stocking. Indian Forester 1981;107:551-62.
11. Selvam AB, Bandyopadhyay S. Fluorescence analysis on the roots of Rauwolfia serpentina (L.) Benth. Ex kurz under UV radiation. Anc Sci Life 2005;24:164-7.
12. Prakash NK, Ranjith M, Sripriya N, Lakshmi RP, Deepa S, Bhuvaneswari S. Antioxidant, free radical scavenging activity and GC-MS studies on Pedilanthus tithymaloides (l.) Poit. Int J Pharm Pharm Sci 2014;6:284-7.
13. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 2011;48:412-22.
14. Al-Henhena N, Ying RP, Ismail S, Najm W, Khalifa SA, El-Seedi H, et al. Chemopreventive efficacy of Andrographis paniculata on azoxymethane-induced aberrant colon crypt foci in vivo. PLoS One 2014;9:1-12.
15. Alhminghlui K, Jagetia GC. Evaluation of the free-radical scavenging and antioxidant activities of Chilauni, Schima wallichii Korth in vitro. Future Sci OA 2018;4:FSO272.
16. Ho PJ, Sung JJ, Cheon KK, Tae HJ. Anti-inflammatory effect of Centella asiatica phytosome in a mouse model of phthalic anhydride induced atopic dermatitis. Phytomedicine 2018;43:110-9.
17. Saleem TM, Azeem A, Dilip C, Sankar C, Prasanth N, Duraisami R. Anti-inflammatory activity of the leaf extracts of Gendarussa vulgaris Nees. Asian Pac J Trop Biomed 2011;1:147-9.
18. Ranasinghe P, Ranasinghe P, Kaushalya WP, Abeysekera M, Premakumara GA, Perera YS, et al. In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts. Pharmacogn Res 2012;4:196-202.
Statistics
7 Views | 14 Downloads
How to Cite
SHARMA, P., and R. L. “INVESTIGATION ON PHARMACOGNOSY AS WELL AS THE ANTIOXIDANT, ANTI-INFLAMMATORY POTENTIAL OF THE KATHA POWDER”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 6, May 2021, pp. 125-32, https://innovareacademics.in/journals/index.php/ajpcr/article/view/41710.
Section
Original Article(s)