PHYTOCHEMICAL SCREENING, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF CASSIA MONTANA HEYNE EX ROTH LEAF EXTRACTS
Objective:The leaf extracts of Cassia montana (Caesalpiniaceae) were being used for leucorrhoea and rheumatic pains used by the certain tribal people in Andhra Pradesh. Thepresent studies focused on the antibacterial, antioxidant activity ofleaf extracts of Cassia montana which provide the scientific basis for its folklore applications in various ailments.
Methods:Ethyl-alcohol and water extracts of C. montana(leaf)were screened for phytochemicals, antimicrobial activityand antioxidant activity byusing in vitro methods. The total phenolics were estimated using Folin-ciocalteu reagent with reference to Gallic acid. Whereas the antioxidants of the extracts was quantified using ammonium molybdate reduction assay with reference to the ascorbic acid. The anti-bacterial activity was studied by disc-diffusion method and the radical scavenging assay by DPPH discoloration method with reference to ascorbic acid.
Results: The leaf extracts were tested for antibacterial, antioxidants and phytochemical screening and found the significantphyto-constituents as steroids, lignins, flavonoids, phenols, glycosides, tannins, cardiac glycosides and reducing sugars in ethanol and aqueous extracts. The tested extracts exhibited significant antibacterial activity. It is also observed that ethanolic extracts are more effective with MIC values at 28 and 32 μg/disc againstB. subtilis and S. aureus followed by K. pneumonia and P. aeruginosa with 80 and 90μg/disc.Total polyphenolsquantified in alcohol and water extracts with reference to the gallic acid is 108 and 267 mg/g dry weight of leaf respectively. Total antioxidants quantified in alcohol and water extracts with reference to the ascorbic acid is70 and 85 mg/g dry weight of leaf respectively. The tested extracts were exhibited strong effect on the discoloration of DPPHindicated the significant scavenging activity.
Conclusion:The present study revealed that the tested extracts were exhibited significant anti-bacterial antioxidant activity along with the diversified phyto-chemicals. Hence the leaf extracts of C. montana were having potential role in the treatment of ethnobotanical health claims.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.