COMPARATIVE IN VITRO ANTIOXIDANT ACTIVITY OF ETHYL ACETATE AND ETHANOL EXTRACTS OF CALLICARPA MACROPHYLLA

  • GANESH N SHARMA Department of Pharmacognosy, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, India.
  • HARJINDER KAUR Department of Pharmacognosy, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, India.
  • BIRENDRA SHRIVASTAVA Department of Pharmacognosy, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, India.
  • SATISH CHANDER ARORA Department of Pharmaceutical Sciences, RKSD College of Pharmacy, Kaithal, Haryana, India.

Abstract

Objective: The objective of the study was to evaluate and compare the antioxidant activity of (EA and E) ethyl acetate and ethanol extracts of (CM) Callicarpa macrophylla.


Methods: The physiochemical parameters were assessed according to guidelines given by the world health organization. The total content of phenols and flavonoids was assessed by Folin–Ciocalteu and aluminum chloride methods. In vitro, antioxidant activity was screened by (DPPH) 1, 1-diphenyl-2- picrylhydrazyl and(H2O2) hydrogen peroxide scavenging and reducing power assay.


Results: The physicochemical parameters fulfilled the standards of WHO guidelines. Total phenol and flavonoid content were more in ethanol extract as compared to ethyl acetate extract of CM. The antioxidant activity of ethanol extract was further high as compared to ethyl acetate extract of Callicarpa macrophylla. The IC50 of Callicarpa macrophylla ethanol extract was less than the ethyl acetate extract. So, more antioxidant activity of ethanol extract compared to ethyl acetate extract of CM.


Conclusion: Overall, both the extracts showed antioxidant activity and can be used further for diseases that can be managed using antioxidants. Ethanol extract possessed significant antioxidant effects than the ethyl acetate extract.

Keywords: Callicarpa macrophylla, Antioxidant activity, Total flavonoid content, Phenol content, Physical evaluation

References

1. Maharaja P, Sengottuvel T, Aarthi A, Gopalasatheeskumar K. Review on antioxidant and hepatoprotective activity of medicinal plants against the paracetamol-induced animal model. Res J Pharmacogn Phytochem 2020;12:114-9.
2. Temple NJ. Antioxidants and disease: More questions than answers. Nutr Res 2000;20:449-59.
3. Yadav V, Jayalakshmi S, Singla RK, Patra A, Khan S. Preliminary assessment of anti-inflammatory activity of Callicarpa macrophylla Vahl. leaves extracts. Indo Glob J Pharm Sci 2011;1:219-22.
4. Yadav V, Jayalakshmi S, Patra A, Singla RK. Investigation of analgesics and antipyretic potentials of Callicarpa macrophylla Vahl. leaves extracts. Int J Med Mol Med 2012;3:1-7.
5. Sheat WG. Propagation of Trees, Shrubs and Conifers. London: Macmillan and Co. Ltd.; 1948.
6. Sharma P, Yelne M, Dennis T. Database on Medicinal Plants Used in Ayurveda. Vol. 3. New Delhi: Central council for research in Ayurveda and Siddha; 2005.
7. Au DT, Wu J, Jiang Z, Chen H, Lu G, Zhao Z. Ethnobotanical study of medicinal plants used by Hakka in Guangdong, China. J Ethnopharmacol 2008;117:41-50.
8. Verma T, Chandrashekar KS, Joshi AB. ?-Amyrin caprylate-a new triterpene isolated from the leaf of Bauhinia purpurea Linn. Asian J Res Chem 2009;2:569-70.
9. World Health Organization. Guidelines for the Assessment of Herbal Medicines. Geneva: World Health Organization;1991.
10. Kokate CK, Purohit AP, Gokhale SB. Pathway to screen phytochemical nature of natural drugs. In: Pharmacognosy. 39th ed. India: Nirali Prakashan; 2007. p. 607-11.
11. Raj B, John S, Abdul RA. Pharmacognostic and preliminary phytochemical screening of Ampelocissus indica including antioxidant activity. Res J Pharmacogn Phytochem 2020;12:80-2.
12. Biswas A, Dey S, Li D, Yiu L, Zhang J, Huang S, et al. Comparison of the phytochemical profile, mineral content, and in vitro antioxidant activities of Corchorus capsularis and Corchorus olitorius leaf extracts from different populations. J Food Qual 2020;2020:1-14.
13. Woisky RG, Salatino A. Analysis of propolis: Some parameters and procedures for chemical quality control. J Apicult Res 1998;37:99-105.
14. Bozin B, Mimica-Dukic N, Samojlik I, Goran A, Igic R. Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chem 2008;111 Suppl. 4:925-9.
15. Renuka K, Devi VR, Subramanian SP. Phytochemical screening and evaluation of in vitro antioxidant potential of immature palmyra palm (Borassus flabellifer Linn.) fruits. Int J Pharm Pharm Sci 2018;10:77-83.
16. Ruba AA, Mohan VR. In vitro antioxidant potential of the whole plant of Andrographis echioides (l.) Nees (Acanthaceae). Int J Pharm Pharm Sci 2016;8:70-75.
17. Sharma HK, Gogoi B, Nainwal LM. A review on some antioxidant plant species growing in North East India. Int J Pharm Sci Rev 2016;37:224-9.
18. Zohera FT, Habib MR, Imam MZ, Mazumder ME, Rana MS. Comparative antioxidant potential of different extracts of Celastrus paniculatus Willd seed. S J Pharm Sci 2010;1:68-74.
Statistics
54 Views | 85 Downloads
Citations
How to Cite
N SHARMA, G., H. KAUR, B. SHRIVASTAVA, and S. CHANDER ARORA. “COMPARATIVE IN VITRO ANTIOXIDANT ACTIVITY OF ETHYL ACETATE AND ETHANOL EXTRACTS OF CALLICARPA MACROPHYLLA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 14, no. 5, May 2021, pp. 31-35, doi:10.22159/ajpcr.2021.v14i5.41014.
Section
Original Article(s)