TOTAL AND DIFFERENTIAL LEUKOCYTE COUNT IN HEALTHY NEWBORNS
Keywords:Thin-layer chromatography, Differential leukocyte count,, Gestation, Newborns
Objectives: The objectives of the study were to establish normal reference values of total and differential leukocyte count (DLC) in Indian neonatal population.
Methods: The study was conducted on 200 healthy newborns admitted to neonatology section of Department of Paediatricsa. Maternal and Neonatal data was recorded on a pretested pro forma. Thin-layer chromatography (TLC) was done by manual method (hemocytometer method) using improved Neubauer Chamber. For calculating DLC smear was made from the EDTA blood and was stained by Leishman stain (Romanowsky group). DLC was done in body and tail part of the stained smears by following Z pattern.
Statistical Analysis: Descriptive statistical analysis was carried out in the present study. Mean, standard deviation, t-test, and analysis of variance tests were used to test the significance of the differences among sample means.
Results: Mean TLC at various period of gestations were 4500/cumm at 31 weeks, 7627±2320/cumm at 33 weeks, 8325±1943/cumm at 34 weeks, 8141±1538/cumm at 35 weeks, 8455±904/cumm at 36 weeks, 8840±658/cumm at37 weeks, 9380±896/cumm at 38 weeks, 10143±1369/cumm at 39 weeks, 9155±2444/cumm at 40 weeks, and 11750±353/cumm at 41 weeks.
Conclusion: TLC showed increase with gestation except at 35 weeks and 40 weeks. Neutrophils shows increase with gestation except at 35 weeks and 40 weeks lymphocytes shows increase with gestation except at 34 weeks, 39 weeks, and 40 weeks.
Ashavaid TF, Todur SP, Dherai AJ. Establishment of reference intervals in Indian population. Indian J Clin Biochem 2005;20:110-8. doi: 10.1007/BF02867409, PMID 23105542.
Aneja S, Manchanda R, Patwari A, Sagreiya K, Bhargava SK. Normal hematological values in newborns. Indian Pediatr 1979;16:781-6. PMID 536019.
Noguera NI, Detarsio G, Pérez SM, Bragós IM, Lanza O, Rodríguez JH, et al. Hematologic study of newborn umbilical cord blood. Medicina (B Aires) 1999;59:446-8. PMID 10684163.
Schelonka RL, Yoder BA, desJardins SE, Hall RB, Butler J. Peripheral leukocyte count and leukocyte indexes in healthy newborn term infants. J Pediatr 1994;125:603-6. doi: 10.1016/s0022-3476(94)70018-4, PMID 7931882.
Borna H, Borna S, Rafati SH. Umbilical cord hematologic variables in different modes of delivery. Tehran Univ Med J 2006;64:49-56.
Proytcheva MA. Issues in neonatal cellular analysis. Am J Clin Pathol 2009;131:560-73. doi: 10.1309/AJCPTHBJ4I4YGZQC, PMID 19289592.
Rennie JM. Roberton’s Textbook of Neonatology. 2005;4:751.
Qaiser DH, Sandila MP, Ahmed ST, Kazmi T. Haematological reference values for full term, healthy, newborns of Karachi, Pakistan. J Pak Med Assoc 2009;59:618-22. PMID 19750858.
Sood R. Examination of peripheral blood film. Med Lab Technol 1994;4:188-94.
Manroe BL, Weinberg AG, Rosenfeld CR, Browne R. The neonatal blood count in health and disease. I. Reference values for neutrophilic cells. J Pediatr 1979;95:89-98. doi: 10.1016/s0022-3476(79)80096-7, PMID 480023.
Weinberg AG, Rosenfeld CR, Manroe BL, Browne R. Neonatal blood cell count in health and disease. II. Values for lymphocytes, monocytes, and eosinophils. J Pediatr 1985;106:462-6. doi: 10.1016/s0022- 3476(85)80681-8, PMID 4038739.
Coulombel L, Dehan M, Tchernia G, Hill C, Vial M. The number of polymorphonuclear leukocytes in relation to gestational age in the newborn. Acta Paediatr Scand 1979;68:709-11. doi: 10.1111/j.1651- 2227.1979.tb18443.x, PMID 525339.
Davies NP, Buggins AG, Snijders RJ, Jenkins E, Layton DM, Nicolaides KH. Blood leucocyte count in the human fetus. Arch Dis Child 1992;67:399-403. doi: 10.1136/adc.67.4_spec_no.399, PMID 1586178.
Nicolaides KH, Thilaganathan B, Mibashan RS. Cordocentesis in the investigation of fetal erythropoiesis. Am J Obstet Gynecol 1989;161:1197-200. doi: 10.1016/0002-9378(89)90664-9, PMID 2589439.
Van den Hof MC, Nicolaides KH. Platelet count in normal, small, and anemic fetuses. Am J Obstet Gynecol 1990;162:735-9. doi: 10.1016/0002-9378(90)90997-l, PMID 2107744.
Xanthou M. Leucocyte blood picture in healthy full-term and premature babies during neonatal period. Arch Dis Child 1970;45:242-9. doi:10.1136/adc.45.240.242, PMID 5419993.
Rosse C, Kraemer MJ, Dillon TL, McFarland R, Smith NJ. Bone marrow cell populations of normal infants: The predominance of lymphocytes. J Lab Clin Med 1977;89:1225-40. PMID 864303.
Abdurrahman MB, Adekoje MA. Haematological values in Northern Nigerian neonates. Trans R Soc Trop Med Hyg 1983;77:786-8. doi: 10.1016/0035-9203(83)90289-4, PMID 6665831.
How to Cite
Copyright (c) 2022 Ashwani Kumar, Meetu Yadav, Gopal Singh Charan
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.