Objective: To develop a reliable, eco-friendly and easy process for the synthesis of silver nanoparticles using fruit extracts of medicinal plant ‘Morinda Tinctoria' and evaluate its anti-microbial properties. Methods: The synthesis and characterization of silver nanoparticles was confirmed by UV–Visible, FR-IR, SEM, TEM, CV techniques and Disc diffusion assay method was used to confirm the antibacterial activity of silver nanoparticles. Results: UV-visible spectrum of the aqueous medium containing silver nanoparticles showed a peak around 420.25nm. FTIR analysis confirmed reduction of Ag+ ions to Ag0 ions in synthesized silver nanoparticles.The SEM and TEM analysis showed the particle size between 17-25nm, and spherical in structure.The silver nanoparticles have shown bactericidal effect against E.Coli, Staphylococus aureus, Bascillus cereus, and Pseudomonas aeruginosa. Conclusions: The fruit extract of M.Tinctoria quickly reduces Ag+ to Ag0 and enhances synthesis of silver nanoparticles with anti-microbial activity.



                            Nanoparticles; Morinda Tinctoria; TEM; Silver nanoparticle, Antibacterial activity;


. Rosei F. Nanostructured surfaces: challenges and frontiers in nanotechnology. J Phys Condens Matter 2004; 16:S1373–S1436.

Papp S, Patakfalvi R, Dekany I. Metal nanoparticle formation on layer silicate lamellae. Colloid Polym Sci 2008; 286:3–14.

Chen D, Qiao X, Qiu X, Chen J. Synthesis and electrical properties of uniform silver nanoparticles for electronic applications. J Mater Sci 2009; 44:1076–1081.

Foglia S, Suber L, Righini M, Size tailoring of CdS nanoparticles by different colloidal chemical techniques,Colloids Surf. A 2001; 177(1):3-12.

Liu J, He F, Gunn TM, Zhao D, Roberts CB. Precise seed-mediated growth and size-controlled synthesis of palladium nanoparticles using a green chemistry approach. Langmuir 2009;25:7116–7128.

Mohanpuria P, Rana Nisha K and Yadav SK. Biosynthesis of nanoparticles: technological concepts and future applications. J. Nanopart. Res 2008; 10:507-517.

Li L, Liu C, Liu Z, Tsao R, Liu S. Identification of phenylethanoid glycosides in plant extract of Plantago asiatica L. by liquid chromatography-electrospray ionization mass spectrometry. Chinese J. Chem 2009; 27:541-545.

Padmadhas R, Ragunathan R. Effect of lead nano particle present in the leaf of Calotrphis Gigantea which result in the loss of the painted grass hoppe of the Western ghats species in India. Int, J. Nanotechnol. Appl 2009; 3: 89-96.

Geoprincy G, Vidhya srri BN, Poonguzhali U, Nagendra Gandhi N, Renganathan S. A Review on Green Synthesis of Silver nanoparticles. Asian J Pharm Clin Res 2013;6(1): 8-12.

Meena N , Jeya M, Aroumugame S, Arumugam P and Sagadevan E. Green synthesis of silver nanoparticles using leaves of lepisanthes tetraphylla and evaluation of their antibacterial activity against drug resistant clinical isolates. Int J Pharm Bio Sci 2012; 3(2): 592-601.

Vivek M, Senthil Kumar P, Steffi S, Sudha S. Biogenic silver nanoparticles by Gelidiella acerosa extract and their antifungal effects .Avicenna. J.Med.Biotech 2011; 3(3): 143-148.

Vilchis-Nestor A, Sa´nchez-Mendieta V, Camacho-Lo´ pez M, Go´ mez-Espinosa R, Camacho-Lo´ pez M, Arenas-Alatorre. Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extract. J. Mater. Lett 2008; 62: 3103.

Narayanan KB, Sakthivel NJ. Coriander leaf mediated biosynthesis of gold nanoparticles. Mater. Lett 2008; 68: 4588-4590.

Saxena A, Tripathi RM, Zafar F, Singh P. Green synthesis of silver nanoparticles using aqueous solution of Ficus benghalensis leaf extract and characterization of their antibacterial activity. Mater Lett 2012; 67: 91–94.

Kaviya S, Santhanalakshmi J, Viswanathan B. Biosynthesis of silver nano-flakes by Crossandra infundibuliformis leaf extract. Mater Lett 2012; 67: 64–66.

Asmita J Gavhane, Padmanabhan P, Suresh P Kamble , Suresh N Jangle. Synthesis of silver nanoparticles using extract of neem leaf and triphala and evaluation of their antimicrobial activities. Int J Pharm Bio Sci 2012; 3(3): P 88 – 100.

Alagumuthu G, Kirubha R.Green synthesis of silver nanoparticles using Cissus quadrangularis plant extract and their antibacterial activity. Int. J. of Nanomater. Bio2012; 2(3) :30-33.

Jancy Mary E , Inbathamizh L. Green synthesis and characterization of nano silver using leaf extract of Morinda Pubescens. Asian J Pharm Clin Res 2012;5(1):159-162.

Firdhouse M, Jannathul, Lalitha P. Green Synthesis of Silver nanoparticles using the aqueous extract of Portulaca Oleracea (l.). Asian J Pharm Clin Res2013;6(1):92.

Isse JJ, Falciola L, Mussiri PR, Gennaro A, Chem.Common. 2006; 344-346.

Duran N, Alves OL, De Souza GIH, Esposito E Marcato PD. Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J Biomed Nanotechnol 2007; 3: 203-208.

Chen JC, Lin ZH, Ma XX. Evidence of the production of silver nanoparticles via pretreatment of Phoma sp. 3.2883 with silver nitrate. Lett Appl Microbiol 2003; 37: 105-108.

Ingle A, Gade A, Pierrat S, Sonnichsen C, Rai M. Mycosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria. Curr Nanosci 2008; 4: 141-144.

Furno F, Morley KS, Wong B, Sharp BL, Arnold PL, Howdle SM, et al. Silver nanoparticles and polymeric medical devices: a new approach to prevention of infection. J Antimicrob Chemother 2008; 54:1019- 24.

Abuskhuna S, Briody J, McCann M, Devereux M, Kavanagh K, Fontecha JB, et al. Synthesis, structure and anti-fungal activity of dimeric Ag(I) complexes containing bis-imidasole ligands. Polyhedron 2004; 23:1249- 5.

Hamouda T, Myc A, Donovan B, Shih A, Reuter JD, Baker Jr JR. A novel surfactant nanoemulsion with a unique non-irritant topical antimicrobial activity against bacteria, enveloped viruses and fungi. Microbiol Res 2000; 156:1 -7.

Crabtree JH, Burchette RJ, Siddiqi RA, Huen IT, Handott LL, Fishman A. The efficacy of silver-ion implanted catheters in reducing peritoneal dialysis-related infections. Perit Dial Int 2003; 23(4): 368- 74.

Russel AD, Hugo WB. Antimicrobial activity and action of silver. Prog Med Chem1994; 31:351- 70.

Marsh PD. Microbiological aspects of the chemical control of plaque and gingivitis. J Dent Res 1992; 71:1431- 8.



How to Cite

R.KIRUBHA, and G.ALAGUMUTHU. “MORINDA TINCTORIA FRUIT ASSISTED BIOSYNTHESIS OF”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 4, Oct. 2013, pp. 60-64,