FREE RADICAL SCAVENGING AND NOS ACTIVATION PROPERTIES OF WATER SOLUBLE CRUDE POLYSACCHARIDE FROM PLEUROTUS OSTREATUS
The aim of this study was to investigate the antioxidant activity using different in vitro assays and nitric oxide synthase activation property of crude water soluble polysaccharide from P. ostreatus. The polysaccharide was isolated by hot water extraction and physico-chemical investigation revealed that it contained high amount of carbohydrate (mostly Î²-glucan) and low amount of protein and phenolic compounds. EC50 values for scavenging of hydroxyl radical and superoxide radical as well as chelating ability of ferrous ion were 665, 390 and 370 Âµg/ml respectively. The polysaccharide also showed potential nitric oxide synthase activation properties. This mushroom might be a good source of bioactive compounds.
Halliwell B. Establishing the significance and optimal intake of dietary antioxidants: the biomarker concept. Nutr Rev 1999; 57: 104-13.
Rai M, Biswas G, Mandal SC, Acharya K. Free radicals and human diseases. In: Mandal SC, editor. Herbal Drugs: A modern approach to understand them better. Kolkata, India: New Central Book Agency (P) Ltd; 2011. p. 479-96.
Wijk RV, Wijk EPV, Wiegant FA, Ives J. Free radicals and low level photon emission in human pathogenesis: State of the art. Indian J Exp Biol 2008; 46: 273-309.
Sasaki YF, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K, Taniguchi K, Tsuda S. The comet assay with 8 mouse organs: Results with 39 currently used food additives. Mutat Res-Gen Tox En 2002; 519: 103-09.
Achike FI, Kwan CY. Nitric oxide, human diseases and the herbal products that affect the nitric oxide signalling pathway. Clin Exp Pharmacol Physiol 2003; 30: 605-15.
Ignaro LJ. Nitric Oxide Biology and Pathobiology. San Diego, CA: Academic Press; 2000.
Sinha AK, Acharya K, Bhattacharya S, Patra SC, Guha M, Ray U et al. Neutralization of antineoplastinâ€ of insulin activated nitric oxide synthase antibody and its effects in cancers. J Cancer Res Clin Oncology 2002; 128: 659-68.
Malinski T. Understanding nitric oxide physiology in the heart: a nano-medical approach. Am J Cardiol 2005; 96:13-24.
Sadler M. Nutritional properties of edible fungi. Nutr Bull 2009; 28: 305-308.
Acharya K. Medicinal properties of mushroom. In: Acharya SN Thomas JE, editors. Advances in Medicinal Plant Research. Kerala, India: Research Signpost; 2007. p. 215-36.
Biswas G, Rana S, Sarkar S, Acharya K. 2011. Cardioprotective activity of ethanolic extract of Astraeus hygrometricus (Pers.) Morg. Pharmacologyonline 2011; 2: 808-17.
Chatterjee S, Dey A, Dutta R, Dey S, Acharya K. Hepatoprotective Effect of the Ethanolic Extract of Calocybe indica on Mice with CCl4Â¬ Hepatic Intoxication. Int J PharmTech Res 2011; 3: 2162-68.
Biswas G, Chatterjee S, Acharya K. 2012. Chemopreventive activity of the ethanolic extract of Astraeus hygrometricus (pers.) morg. on Ehrlichâ€™s Ascites Carcinoma cells. Dig J Nanomater Bios 2012; 7: 185-91.
Acharya K, Chatterjee S, Biswas G, Chatterjee A, Saha GK. Hepatoprotective effect of a wild edible mushroom on carbon tetrachloride-induced hepatotoxicity in mice. Int J Pharm Pharm Sci 2012; 4: 285-88.
Lai TK, Biswas G, Chatterjee S, Dutta A, Pal C, Banerji J, Bhuvanesh N, Reibenspies JH, Acharya K. Leishmanicidal and anticandidal activity of constituents of Indian edible mushroom Astraeus hygrometricus. Chem Biodivers 2012; 9: 1517-24.
Chatterjee S, Biswas G, Chandra S, Saha GK, Acharya K. 2013. Apoptogenic effects of Tricholoma giganteum on Ehrlichâ€™s ascites carcinoma cell. Bioprocess Biosyst Eng 2013; 36: 101-7.
Biswas G, Acharya K. Hypoglycemic activity of ethanolic extract of Astraeus hygrometricus (Pers.) Morg. in alloxan-induced diabetic mice. Int J Pharm Pharm Sci 2013; 5: 391-94.
Chatterjee S, Saha GK, Acharya K. Antioxidant activities of extracts obtained by different fractionation from Tricholoma giganteum basidiocarps. Pharmacologyonline 2011, 3: 88-97.
Acharya K, Chatterjee S, Ghosh S. Comparative evaluation on the free radical scavenging activity of eleven Indian cultivated strains of Pleurotus ostreatus. Pharmacologyonline 2011; 1: 440-50.
Rai M, Acharya K. Proximate composition, free radical scavenging and NOS activation properties of Ramaria aurea. Res J Pharm Technol 2012; 5: 1421-27.
Rai M, Acharya K. Evaluation of antioxidant and nitric oxide synthase activation properties of Volvariella volvacea. Int J Pharm Pharm Sci 2012; 4: 460-63.
Acharya, K., and M. Rai. Proximate composition, free radical scavenging and NOS activation properties of a wild edible mushroom. Int J Pharm Pharm Sci 2013; 4: 67-72.
Patra S, Patra P, Maity KK, Mandal S, Bhunia SK, Dey B et al. A heteroglycan from the mycelia of Pleurotus ostreatus: Structure determination and study of antioxidant properties. Carbohydr Res 2013; 368: 16-21.
Samanta S, Maity K, Nandi AK, Sen IK, Devi KSP, Mukherjee S et al. A glucan from an ectomycorrhizal edible mushroom Tricholoma crassum (Berk.) Sacc.: isolation, characterization and biological studies. Carbohydr Res 2013; 367: 33-40.
Dubois M, Gilles A, Hamitton JK, Reders PA, Smith F. Colorimetric method for determination of sugars related substances. Anal Chem 1956; 28: 350-56.
Bradford MM. A refined sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem 1976; 72: 248-
Ziestin N, Ben-Zaker R. Peroxidase activity presence of phenolic substance in peduncles of rose flowers. Plant Physiol Biochem 1993; 31: 333-39.
Martinez AC, Marcelo EL, Marco AO, Moacyr M. Differential responses of superoxide dismutase in freezing resistant Solanum curtibolum and freezing sensitive Solanum tuberosum subjected to oxidative and water stress. Plant Sci 2001; 160: 505-15.
Halliwell B, Gutteridge JMC, Arumo OI. The deoxyribose method: A simple test tube assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 1987; 165: 215-19.
Dinis TCP, Mudaira VMC, Alnicida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-amino salicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994; 315: 161-69.
Jia L, Bonaventua C, Bonaventura J, Stamler SJ. S-nitrosohemoglobin: A dynamic activity of blood involved in vascular control. Nature 1996; 380: 221-26.
Thetsrimuang C, Khammuang S, Chiablaem K, Srisomsap C, Sarnthima R. Antioxidant properties and cytotoxic properties of crude polysaccharides from Lentinus polychrous Food Chem 2011; 128: 634-39.
Tseng YH, Yang JH, Mau JL. Antioxidant properties of polysaccharides from Ganoderma tsugae. Food Chem 2008; 107: 732-38.
Sun ZW, Zhang LX, Zhang B, Niu TG. Structural characterisation and antioxidant properties of polysaccharides from the fruit bodies of Russula virescens. Food Chem 2010; 118: 675-80.
Kozarski M, Klaus A, NikÅ¡iÄ‡ M, VrviÄ‡ MM, TodoroviÄ‡ N, JakovljeviÄ‡ D, GriensvenicÂ´ LJLDV. Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor J Food Comp Anal 2012; 26: 144-53.
Kozarski M, Klaus A, NikÅ¡iÄ‡ M, Jakovljevic D, Helsper JPFG, Griensven LJLDV. Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus. Food Chem 2011; 129: 1667-75.
He JZ, Ru QM, Dong DD, Sun PL. Chemical Characteristics and Antioxidant Properties of Crude Water Soluble Polysaccharides from Four Common Edible Mushrooms. Molecules 2012; 17: 4373-87.
Kappus H. Lipid peroxidation-mechanism and biological relevance. In: Aruoma OI, Halliwell B editors. Free Radicals and Food Additives. London, UK: Taylor and Francis Press; 1991. p. 59-75.
Aruoma OI, Grootveld M, Halliwell B. The role of iron in ascorbate- dependent deoxyribose degradation. Evidence consistent with a site-specific hydroxyl radical generation caused by iron ions bound to the deoxyribose molecule. J Inorg Biochem 1987; 29: 289-99.
Aruoma OI, Laughton MJ, Halliwell B. Carnosine, homocarnosine and anserine: could they act as antioxidants in vivo? Biochem J 1989; 264: 863-69.
Jayakumar T, Thomas PA, Sheu JR, Geraldine P. In-vitro and in-vivo antioxidant effects of the oyster mushroom Pleurotus ostreatus. Food Res Int 2011; 44: 851-61.
Schmidt HHHW, Lohmann SM, Walter U. The nitric oxide and cGMP signal transduction system - regulation and mechanism of action. Biochim Biophys Acta 1993; 1178: 153-75.
Biswas G, Chatterjee S, Sankar S, Acharya K. Evaluation of antioxidant and nitric oxide synthase activation properties of Astraeus hygriometricus (Pers.) Merg. Intl J Biomed Pharma Sci 2010; 4: 22-6.
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