PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF A WILD EDIBLE MUSHROOM ENTOLOMA LIVIDOALBUM

Authors

  • Adhiraj Dasgupta Molecular and Applied Mycology and Plant Pathology Laboratory Department of Botany University of Calcutta 35, Ballygunge Circular Road Kolkata, 700019 West Bengal (India)
  • Manjula Rai Department of Botany, St. Joseph’s College, Darjeeling, 734104, West Bengal, India.
  • Krishnendu Acharya Molecular and Applied Mycology and Plant Pathology Laboratory Department of Botany University of Calcutta 35, Ballygunge Circular Road Kolkata, 700019 West Bengal (India)

Abstract

Objective: In the course of inventorying and bioprospecting wild edible mushrooms of West Bengal, the ethanolic fraction of Entoloma lividoalbum
was tested for its potential as an antioxidant in vitro and estimate the amounts of putative bioactive compounds present in it.
Methods: The fraction's antioxidant potential was tested by employing various in vitro assay systems, namely, total antioxidant capacity, chelating effect
on ferrous ions, reducing the effect of ferric iron and 1,1-diphenyl-1-picrylhydrazyl radical scavenging assays. Estimation of bioactive components was
carried out following previously established methods.
Results: It was found to be a great reducer of ferric iron, as well as an effective ferrous iron chelator and free radical scavenger. In an attempt to quantify
the bioactive components, the fraction was found to be comprised of mention worthy amounts of phenols, β-carotene, lycopene and flavonoids.
Conclusion: This fraction can be used to treat oxidative stress related ailments.
Keywords: Antioxidant, 1,1-diphenyl-1-picrylhydrazyl, Reducing power, Scavenging activity.

Author Biography

Krishnendu Acharya, Molecular and Applied Mycology and Plant Pathology Laboratory Department of Botany University of Calcutta 35, Ballygunge Circular Road Kolkata, 700019 West Bengal (India)

Molecular and Applied Mycology and Plant Pathology LaboratoryDepartment of Botany

References

Kalac P. Chemical composition and nutritional value of European species

of wild growing mushrooms: A review. Food Chem 2009;113:9‑16.

Pradhan P, Dutta AK, Roy A, Basu SK, Acharya K. Inventory and

spatial ecology of macrofungi in the Shorea robusta forest ecosystem

of lateritic region of West Bengal. Biodiversity 2012;13(2):88‑99.

Khatua S, Paul S, Acharya K. Mushroom as the potential source

of new generation of antioxidant: A review. Res J Pharm Tech

;6(5):496‑505.

Acharya K, Chatterjee S, Ghosh S. Comparative evaluation on the

free radical scavenging activity of eleven Indian cultivated strains of

Pleurotus ostreatus. Pharmacol Online 2011;1:440‑50.

Chatterjee S, Saha GK, Acharya K. Antioxidant activities of extracts

Asian J Pharm Clin Res, Vol 8, Issue 5, 2015, 179-182

Dasgupta et al.

obtained by different fractionation from Tricholoma giganteum

basidiocarps. Pharmacol Online 2011;3:88‑97.

Patra S, Patra P, Maity KK, Mandal S, Bhunia SK, Dey B, et al.

A heteroglycan from the mycelia of Pleurotus ostreatus: Structure

determination and study of antioxidant properties. Carbohydr Res

;368:16‑21.

Asatiani MD, Elisashvili V, Songulashvili G, Reznick AZ, Wasser

SP. Higher basidiomycetes mushrooms as a source of antioxidants.

Progress in Mycology. Netherlands: Springer; 2010. p. 311‑26.

Chatterjee S, Datta R, Dey A, Pradhan P, Acharya K. In vivo

hepatoprotective activity of ethanolic extract of Russula albonigra

against carbon tetrachloride‑induced hepatotoxicity in mice. Res J

Pharm Tech 2012;5(8):1034‑8.

Chatterjee S, Dey A, Datta R, Dey S, Acharya K. Hepatoprotective

effect of the ethanolic extract of calocybe indica on mice with ccl

hepatic intoxication. Int J Pharm Tech Res 2011;3:2162‑8.

Biswas G, Sarkar S, Acharya K. Hepatoprotective activity of the

ethanolic extract of Astraeus hygrometricus (Pers.) Morg. Dig J

Nanomater Biostruct 2011;6:637‑41.

Biswas G, Chatterjee S, Acharya K. Chemopreventive activity of the

ethanolic extract of Astraeus hygrometricus (Pers.) Morg. On Ehrlich’s

ascites carcinoma cell. Dig J Nanomater Biostruct 2012;7:185‑91.

Chatterjee S, Biswas G, Chandra S, Saha GK, Acharya K. Apoptogenic

effects of Tricholoma giganteum on Ehrlich’s ascites carcinoma cell.

Bioprocess Biosyst Eng 2013;36(1):101‑7.

Chatterjee S, Biswas G, Chandra S, Saha GK, Acharya K.

Chemopreventive effect of Tricholoma giganteum against benzo[a]

pyrene‑induced forestomach cancer in Swiss albino mice. Int J Pharm

Sci Rev Res 2014;26(2):189‑96.

Mallick S, Dutta A, Dey S, Ghosh J, Mukherjee D, Sultana SS, et al.

Selective inhibition of Leishmania donovani by active extracts of

wild mushrooms used by the tribal population of India: An in vitro

exploration for new leads against parasitic protozoans. Exp Parasitol

;138:9‑17.

Giri S, Biswas G, Pradhan P, Mandal SC, Acharya K. Antimicrobial

activities of basidiocarps of wild edible mushrooms of West Bengal,

India. Int J Pharm Tech Res 2012;4(4):1554‑60.

Rai M, Sen S, Acharya K. Antimicrobial activity of four wild edible

mushrooms from Darjeeling Hills, West Bengal, India. Int J Pharm

Tech Res 2013;5(3):949‑56.

Biswas G, Acharya K. Hypoglycemic activity of ethanolic extract of

Astraeus hygrometricus (Pers.) Morg. in alloxan‑induced diabetic mice.

Int J Pharm Pharm Sci 2013;5 Suppl 1:391‑4.

Chatterjee A, Khatua S, Chatterjee S, Mukherjee S, Mukherjee A,

Paloi S, et al. Polysaccharide‑rich fraction of Termitomyces eurhizus

accelerate healing of indomethacin induced gastric ulcer in mice.

Glycoconj J 2013;30(8):759‑68.

Patra P, Bhanja SK, Sen IK, Nandi AK, Samanta S, Das D, et al.

Structural and immunological studies of hetero polysaccharide

isolated from the alkaline extract of Tricholoma crassum (Berk.) Sacc.

Carbohydr Res 2012;362:1‑7.

Nandi AK, Sen IK, Samanta S, Maity K, Devi KS, Mukherjee

S, et al. Glucan from hot aqueous extract of an ectomycorrhizal

edible mushroom, Russula albonigra (Krombh.) Fr.: Structural

characterization and study of immunoenhancing properties. Carbohydr

Res 2012;363:43‑50.

Samanta S, Maity K, Nandi AK, Sen IK, Devi KS, Mukherjee S,

et al. A glucan from an ectomycorrhizal edible mushroom Tricholoma

crassum (Berk.) Sacc.: Isolation, characterization, and biological

studies. Carbohydr Res 2013;367:33‑40.

Nandi AK, Structural elucidation of an immunoenhancing heteroglycan

isolated from Russula albonigra (Krombh.) Fr. Carbohydr Polym

;94(2):918‑26.

Nandi AK, Samanta S, Maity S, Sen IK, Khatua S, Devi KS, et al.

Antioxidant and immunostimulant ß‑glucan from edible mushroom

Russula albonigra (Krombh.) Fr. Carbohydr Polym 2014;99:774‑82.

Dasgupta A, Rai M, Acharya K. Chemical composition and antioxidant

activity of a wild edible mushroom Pleurotus flabellatus. Int J Pharm

Tech Res 2013;5(4):1655‑63.

Singleton VL, Rossi JA. Colorimetry of total phenolics with

phosphomolybdic‑phosphotungstic acid reagents. Am J Enol Vitic

;16(3):144‑58.

Adebayo EA, Oloke JK, Ayandele AA, Adegunlola CO. Phytochemical,

antioxidant and antimicrobial assay of mushroom metabolite from

Pleurotus pulmonarius –LAU 09 (JF736658). J Microbiol Biotechnol

Res 2012;2(2):366‑74.

Nagata M, Yamashita I. Simple method for simultaneous determination

of chlorophyll and carotenoids in tomato fruit. Nippon Shokuhin Kogyo

Gakkaishi 1992;39(10):925‑8.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of

antioxidant capacity through the formation of a phosphomolybdenum

complex: Specific application to the determination of vitamin E. Anal

Biochem 1999;269(2):337‑41.

Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative

properties of xanthan on the autoxidation of soybean oil in cyclodextrin

emulsion. J Agric Food Chem 1992;40(6):945‑8.

Dinis TC, Maderia VM, Almeida LM. Action of phenolic derivatives

(acetaminophen, salicylate, and 5‑aminosalicylate) as inhibitors of

membrane lipid peroxidation and as peroxyl radical scavengers. Arch

Biochem Biophys 1994;315(1):161‑9.

Oyaizu M. Studies on product of browning reaction prepared from

glucose amine. Jpn J Nutr 1986;44:307‑15.

Jayakumar T, Thomas PA, Geraldine P. In-vitro antioxidant activities of

an ethanolicextract of the oyster mushroom, Pleurotus ostreatus. Innov

Food Sci Emerg Technol 2009;10(2):228‑34.

Pal J, Ganguly S, Tahsin KS, Acharya K. In vitro free radical scavenging

activity of wild edible mushroom, Pleurotus squarrosulus (Mont.)

Singer. Indian J Exp Biol 2010;48(12):1210‑8.

Lee YL, Huang GW, Liang ZC, Mau JL. Antioxidant properties of

three extracts from Pleurotus citrinopileatus. LWT Food Sci Technol

;40(5):823‑33.

Sowndhararajan K, Joseph JM, Manian S. Antioxidant and free radical

scavenging activities of indian acacias: Acacia Leucophloea (Roxb.)

Willd., Acacia Ferruginea Dc., Acacia Dealbata Link. and Acacia

Pennata (L.) Willd. Int J Food Prop 2013;16(8):1717‑29.

Jayaprakasha GK, Girennavar B, Patil BS. Radical scavenging activities

of Rio Red grapefruits and Sour orange fruit extracts in different in vitro

model systems. Bioresour Technol 2008;99:4484‑94.

Dasgupta A, Ray D, Chatterjee A, Roy A, Acharya K. In vitro

antioxidative behaviour of ethanolic extract of Russula albonigra.

J Chem Pharm Res 2014;6(3):1366‑72.

Cheung LM, Cheung PC, Ooi VE. Antioxidant activity and total

phenolics of edible mushroom extracts. Food Chem 2003;81:249‑55.

Lee YL, Yen MT, Mau JL. Antioxidant properties of various extracts

from Hypsizigus marmoreus. Food Chem 2007;104:1‑9.

Chatterjee S, Saha GK, Acharya K. Antioxidant activities of extracts

obtained by different fractionation from Tricholoma giganteum

basidiocarps. Pharmacology 2011;3:88‑97.

Vaz AJ, Barros L, Martins A, Santos‑Buelga C, Vasconcelos HM,

Ferreira IC. Chemical composition of wild edible mushrooms and

antioxidant properties of their water soluble polysaccharidic and

ethanolic fractions. Food Chem 2011;126(2):610‑6.

Published

2015-09-01

How to Cite

Dasgupta, A., M. Rai, and K. Acharya. “PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF A WILD EDIBLE MUSHROOM ENTOLOMA LIVIDOALBUM”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 5, Sept. 2015, pp. 171-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/7176.

Issue

Section

Original Article(s)