ISOLATION OF PHYTOCONSTITUENTS AND IN VITRO ANTILITHIATIC BY TITRIMETIC METHOD, ANTIOXIDANT ACTIVITY BY 1,1- DIPHENYL -2- PICRYL HYDRAZYL SCAVENGING ASSAY METHOD OF ALCOHOLIC ROOTS & RHIZOMES EXTRACT OF HEDYCHIUM CORONARIUM J. KOENIG PLANT SPECIES
Objective: The herbal medicines do not have any side effect and it is cost effective and safest medicine from an ancient periods. Urolithiasis or Lithiasis
is a consequence of complex physical processes. The major factors are supersaturation of urine with the offending salt and crystallization. Crystals
retained in kidney can become nucleus for stone formation. This process is synonymously known as Urolithiasis or Lithiasis or Nephrolithiasis or
Kidney stones or renal calculi. Calcium-containing stones are the most common comprising about 75% of all urinary calculi, which may be in the
form of pure calcium oxalate (50%) or calcium phosphate (5%) and a mixture of both (45%). In this study, ethanolic & aqueous extracts of roots and
rhizomes of Hedychium coronarium J. Koenig plant were evaluated for their potential to dissolve experimentally prepared kidney stones like calcium
oxalate by titrimetic method with an invitro model and Antioxidant activity performed by DPPH scavenging assay method. Phytoconstituents were
also isolated by chromatographic techniques of this plant species.
Method: For performing invitro antilithiatic activity titrimetic method was adopted and for antioxidant activity 1,1-diphenyl-2-picryl hydrazyl
(DPPH) scavenging assay method was adopted. Phytoconstituents were isolated by column and thin layer chromatographic techniques.
Results & Conclusion: Ethanolic roots & rhizomes extract of this plant produced highest dissolution of stones when compare to standard drug
cystone and at 10 mg. concentration. Also this study showed alcoholic extract of roots & rhizomes of Hedychium coronarium J. Koenig plant in higher
concentration possess best antioxidant potential as compare to standard ascorbic acid with IC50 value 9.0 and 18.9 Î¼g/ml. for ascorbic acid and
alcoholic extract respectively. Isolated phytoconstituent from alcoholic extract of this plant was 8a, hydroxy hedychilactone and its structure was
confirmed by IR, NMR and Mass spectroscopic datas.
Keywords: Hedychium coronarium J. Koenig, Urolithiasis, Kidney stones, Calcium oxalate, Antioxidants, 1,1- diphenyl-2-picryl hydrazyl etc.
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Table 1: Calcium oxalate dissolution by extracts and cystone
Volume of standard KMnO4 (ml)
Weight of calcium estimated (mg)
Weight of calcium reduced (mg)
Percentage dissolution (%)
Alcoholic extract of roots and rhizomes
Aqueous extract of roots and rhizomes
Correspond to 10 mg
Table 2: Absorbance of different extract of H. coronarium J. Koenig with ascorbic acid
Ascorbic acid (Abs)
Average control reading: 0.2444, % inhibition=(Abscontrolâˆ’Abstest)/AbscontrolÃ—100
Table 3: % inhibition of different extract of H. coronarium J. Koenig
Ascorbic acid (% inhibition)
PEE (% inhibition)
CHCl3 E (% inhibition)
AlcE (% inhibition)
H. coronarium: Hedychium coronarium
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