• Ratikanta Tripathy Assistant Professor, Dept. of Pharmacology, KIMS, BBSR, 751024
  • Manas Ranjan Pradhan
  • Basudev Lenka
  • Manjushree Mohanty


Objectives: To evaluate the cardiotonic action of aqueous extract of the peel of Citrus sinensis and also to know the effect in the presence of propranolol,
nifedipine, and verapamil.
Methods: We studied the cardiotonic effect of citrus peel extracts on isolated frog heart perfusion technique alone and also with a continuous infusion
with calcium channel blockers and a beta blocker. The aqueous extract obtained by drying the peel of citrus and then adding the powder form in
distilled water.
Results: Aqueous extract produced positive ionotropic action on frog heart and its effect antagonized by propranolol but neither by nifedipine nor
Conclusion: Aqueous extract of peels of C. sinensis has cardiotonic activity and the cardiotonic effect produced by citrus may be due to having beta
adrenergic action.
Keywords: Digoxin, Cardiac stimulant, Isolated frog's heart, Citrus sinensis.

Author Biography

Ratikanta Tripathy, Assistant Professor, Dept. of Pharmacology, KIMS, BBSR, 751024
Assistant Professor, Dept. of Pharmacology


1. Available from: http://www.who.int/mediacenter/factsheets/fs317/en/.
2. Beller GA, Smith TW, Abelmann WH, Haber E, Hood WB Jr. Digitalis
intoxication. A prospective clinical study with serum level correlations.
N Engl J Med 1971;284(18):989-97.
3. Kitada Y, Narimatsu A, Suzuki R, Endoh M, Taira N. Does the
positive inotropic action of a novel cardiotonic agent, MCI-154,
involve mechanisms other than cyclic AMP? J Pharmacol Exp Ther
4. Suchalatha S, Thirugnanasambandam P, Maheswaran E, Shyamala
Devi CS. Role of Arogh, a polyherbal formulation to mitigate oxidative
stress in experimental myocardial infarction. Indian J Exp Biol
5. Ekwenye UN, Edeha OV. The antibacterial activity of crude leaf extract
of Citrus sinensis sweet orange. Int J Pharm Biol Sci 2010;1:742.
6. Parmar HS, Kar A. Antidiabetic potential of Citrus sinensis and
Punica granatum peel extracts in alloxan treated male mice. Biofactors
7. Oliveira ED, Leite TS, Silva BA, Conde-Garcia EA. Inotropic effect of
Citrus sinensis (L.) Osbeck leaf extracts on the guinea pig atrium. Braz
J Med Biol Res 2005;38(1):111-8.
8. Parmar HS, Kar A. Antiperoxidative, antithyroidal, antihyperglycemic
and cardioprotective role of Citrus sinensis peel extract in male mice.
Phytother Res 2008;22(6):791-5.
9. Asgary S, Keshvari M. Effects of Citrus sinensis juice on blood
pressure. ARYA Atheroscler 2013;9(1):98-101.
10. Bachhav KV, Burande MD, Rangari VD, Mehta JK. Effect of aqueous
extract of Ipomoea carnea leaf on isolated frog and mouse heart. Indian
J Exp Biol 1999;37(11):1080-4.
258 Views | 361 Downloads
How to Cite
Tripathy, R., M. R. Pradhan, B. Lenka, and M. Mohanty. “EFFECT OF AQUEOUS EXTRACT OF PEEL OF CITRUS SINENSIS ON FROG’S HEART”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 1, Jan. 2016, pp. 84-86, https://innovareacademics.in/journals/index.php/ajpcr/article/view/8546.
Original Article(s)