SACCHAROMONOSPORA OCEANI VJDS‑3, A POTENT ACTINOBACTERIAL STRAIN FROM MANGROVE ECOSYSTEM

  • Mani Deepa Indupalli Department of Botany and Microbiology Acharya Nagarjuna University Guntur, AP India
  • Vijayalakshmi Muvva Department of Botany and Microbiology Acharya Nagarjuna University Guntur, AP India
  • Rajesh Kumar Munaganti Department of Botany and Microbiology Acharya Nagarjuna University Guntur, AP India
  • Usha Kiranmayi Mangamuri Department of Botany and Microbiology Acharya Nagarjuna University Guntur, AP India

Abstract

Objective: The aim of the present study was to isolate, identify, and analyze the phylogenetic characteristics of a rare actinobacterial strain VJDS‑3
with antagonistic activities isolated from Mangrove ecosystems of Nizampatnam, Guntur District, Andhra Pradesh, India.
Methods: Soil samples collected were pre‑treated with calcium carbonate and used for isolation of potent actinobacterial strain designated as VJDS‑3.
Identification of the strain was carried out by studying the micro‑morphological, cultural, biochemical, and physiological methods. The phylogenetic
study of the strain was carried out by employing 16S rDNA sequence‑based analysis. The phylogenetic tree was constructed using the Molecular
Evolutionary Genetic Analysis software version 6.
Results: The potent actinobacterial strain was identified as Saccharomonospora oceani VJDS‑3, and the bioactive metabolites produced by the strain
inhibited Gram‑positive bacteria (Bacillus megaterium, Bacillus subtilis), Gram‑negative bacteria (Xanthomonas campestris, Pseudomonas aeruginosa,
Proteus vulgaris, and Escherichia coli), and fungi (Aspergillus niger, Botrytis cinerea, Fusarium solani, Fusarium Oxysporum, and Candida albicans).
Conclusion: The results of the experiment showed that the crude ethyl acetate extract of S. oceani VJDS‑3 showed significant antimicrobial potential,
and hence it can be used for isolation of compounds with pharmaceutical importance.
Keywords: Mangrove ecosystems, Phylogenetic study, Saccharomonospora oceani VJDS‑3, Bioactive compounds.

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How to Cite
Indupalli, M. D., V. Muvva, R. K. Munaganti, and U. K. Mangamuri. “SACCHAROMONOSPORA OCEANI VJDS‑3, A POTENT ACTINOBACTERIAL STRAIN FROM MANGROVE ECOSYSTEM”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 1, Jan. 2016, pp. 370-3, https://innovareacademics.in/journals/index.php/ajpcr/article/view/9069.
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