SACCHAROMONOSPORA OCEANI VJDSâ€‘3, A POTENT ACTINOBACTERIAL STRAIN FROM MANGROVE ECOSYSTEM
Objective: The aim of the present study was to isolate, identify, and analyze the phylogenetic characteristics of a rare actinobacterial strain VJDSâ€‘3
with antagonistic activities isolated from Mangrove ecosystems of Nizampatnam, Guntur District, Andhra Pradesh, India.
Methods: Soil samples collected were preâ€‘treated with calcium carbonate and used for isolation of potent actinobacterial strain designated as VJDSâ€‘3.
Identification of the strain was carried out by studying the microâ€‘morphological, cultural, biochemical, and physiological methods. The phylogenetic
study of the strain was carried out by employing 16S rDNA sequenceâ€‘based analysis. The phylogenetic tree was constructed using the Molecular
Evolutionary Genetic Analysis software version 6.
Results: The potent actinobacterial strain was identified as Saccharomonospora oceani VJDSâ€‘3, and the bioactive metabolites produced by the strain
inhibited Gramâ€‘positive bacteria (Bacillus megaterium, Bacillus subtilis), Gramâ€‘negative bacteria (Xanthomonas campestris, Pseudomonas aeruginosa,
Proteus vulgaris, and Escherichia coli), and fungi (Aspergillus niger, Botrytis cinerea, Fusarium solani, Fusarium Oxysporum, and Candida albicans).
Conclusion: The results of the experiment showed that the crude ethyl acetate extract of S. oceani VJDSâ€‘3 showed significant antimicrobial potential,
and hence it can be used for isolation of compounds with pharmaceutical importance.
Keywords: Mangrove ecosystems, Phylogenetic study, Saccharomonospora oceani VJDSâ€‘3, Bioactive compounds.
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