TARGETED THERAPY FOR BREAST CANCER CELLS BY HERBAL DRUG FORMULATIONS OF IRON OXIDE NANOPARTICLES

  • Praseetha P.k Noorul Islam University
  • Swathy J.s Noorul Islam University
  • Sakthivel G Noorul Islam University

Abstract

Nanoparticle based drug delivery is the currently focused area in case of various therapeutic and diagnostic techniques. Iron oxide nanoparticles are used as carriers in drug delivery due to their unique properties and negligible side effects. We analyze the potential therapeutic properties of methanolic extracts of Centella asiatica loaded with PVP iron oxide nanoparticles against human breast cancer cell lines MCF-7 and the results were compared with normal cell lines. Characterization of coated nanoparticles was done by UV-VIS spectroscopy, FTIR spectroscopy and Particle size analyzer. Loading of herbal drug extract to nanoparticles was confirmed using TEM, Particle size analyzer, FTIR and Biochemical assays. Initially, dose depended hydrogen per oxide free radical scavenging activity were recorded. Methanolic extracts of Centella asiatica showed 55, 59, 71 and 80% inhibition respectively. MTT assay was performed to estimate the drug loading efficiency for PVP coated iron oxide nanoparticle at 1hr and 24 hrs. At 10µl concentration methanolic extracts of Centella asiatica loaded PVP coated iron oxide nanoparticles exhibited significant cell necrosis in breast cancer cell line MCF-7 as 60%. In case of normal cell lines L929, the cytotoxicity was reduced to 10.42 respectively. This work was aimed to increase the efficiency, reduce side effects of chemotherapeutic drugs and provide conveniences for the future therapeutics by coupling herbal drug with polymer coated nanoparticles. 

Author Biographies

Praseetha P.k, Noorul Islam University

Nanotechnology

Associate Professor and Head

Swathy J.s, Noorul Islam University

Nanotechnology

M.Tech Student

Sakthivel G, Noorul Islam University

Nanotechnology

Ph.D Scholar

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How to Cite
P.k, P., S. J.s, and S. G. “TARGETED THERAPY FOR BREAST CANCER CELLS BY HERBAL DRUG FORMULATIONS OF IRON OXIDE NANOPARTICLES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 1, Jan. 2016, pp. 347-53, https://innovareacademics.in/journals/index.php/ajpcr/article/view/9970.
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