• Sri Agung Fitri Kusuma Department of Biology Pharmacy, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia 45363,
  • Soraya Ratnawulan Mita Departement of Pharmaceutics, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia 45363.
  • Ratna Fitria Ermawati Department of Biology Pharmacy, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia 45363,


Objective: The objective of this study was to determine the best ratio of maltodextrin and extract concentration on performance of anti-dysentery granule containing Klutuk banana fruit extract (Musa balbisiana Colla) as an effective antimicrobial to treat dysentery caused by Shigella dysenteriae and combined with its pseudostem extract to supply potassium needed for supporting dehydration impact caused by dysentery.
Methods: The dried fruit and pseudostem of the Klutuk banana plant were each extracted by maceration method. Each granule formula was optimized in different ratio of extract and maltodextrin concentration (1:2 (F1); 1:3 (F2); and 1:4 (F3) respectively. Then, the anti-shigellosis granule were formulated using the wet granulation method and evaluated for 30 d. The appearance of the granule, weight variation, loss on drying value, flowability, granule solubility, disintegration time, pH, and anti-dysentery activity of each formula was observed. The potassium content determination of each granule formula was done using an atomic absorption spectrophotometer method.
Results: All formulated granules showed good flow properties and antidysentery activity. Concerning to the solubility, maltodextrin addition showed the increasing solubility of all formulated granule. The F3 achieved the best-improved granule characteristic and had good anti-dysentery effectivity, but had the lowest potassium content (0.362 g/l) among all formulas. The potassium content of F1 and F2 were 0.625 g/l and 0.444 g/l, respectively.
Conclusion: Maltodextrin can improve the usefulness of granule that containing the Klutuk banana fruit and its pseudostem extracts in dysentery treatment and the dehydration impact.

Keywords: Musa balbisiana Colla, fruit, extract, granule, maltodextrin, anti-dysentery


1. Niyogi S. Shigellosis. J Microbiol 2005;43:133–43.
2. Bhattacharya SK, Sur D. An evaluation of current shigellosis treatment. Expert Opin Pharmacother 2003;4:1315-20.
3. Yu CE, Lougee DA, Murno JR. Diarrhea and dehydration. Pediatric Education in Disasters Manual. Service Point S. A, Buenos Aires; 2009. p. 189-94.
4. Nafianti S, Sinuhaji AB. Resisten trimetoprim-sulfametoksazol terhadap shigellosis. Sari Pediatri 2005;7:39-44.
5. World Health Organization Pathopysiology of Watery Diarrhoea: Dehydration and Rehydration. Geneva; 1992.
6. Lima AAM, Lima NL, Pinho MC. High frequency of strain multiply resistant to ampicillin, trimethoprim-sulfametoksazol, streptomycin, subject: chloramphenicol, and metracycline isolated from a patient with shigellosis in Northeastern Brazil during period 1988 to 1993. Antimicrob Agents Chemother 1995;9:256-79.
7. WHO. The treatment of diarrhoea: a manual for physicians and other senior health workers. Geneva; 2005.
8. Sivapalasingam S, Nelson JM, Joyce K, Hoekstra M, Angula FJ, Mintz ED. High prevalence of antimicrobial resistance among Shigella isolates in the United States tested by the National Antimicrobial Resistance Monitoring System from 1999 to 2002. Antimicrob Agents Chemother 2006;50:49–54.
9. Dutta S, Ghosh A, Ghosh K, Dutta D, Bhattacharya SK, Nair GB. Newly emerged multiple antibiotic-resistant Shigella dysenteriae type 1 strains in and around Kolkatta, India, are clonal. J Clin Microbiol 2003;41:5833–4.
10. Clark AM. Natural products as a resource for new drugs. Pharm Res 1996;13:1133–41.
11. Cordell GA. Biodiversity and drug discovery a symbiotic relationship. Phytochem 2000;55:463–80.
12. Goleniowski ME, Bongiovanni GA, Bongiovanni L, Palacio CO, Cantero JJ. Medicinal plants from the sierra de comechingones, argentina. J Ethnopharmacol 2006;107:324-41.
13. WHO. Report of the second meeting of Directors of WHO Collaborating centres for traditional medicine. Geneva. Swiazerland; 1987.
14. Ghani A. Medicinal plants of bangladesh: chemical constituents and uses: 2003. 2nd ed. The Asiatic Society of Bangladesh, Dhaka; 2003. p. 315.
15. Khare CP. Indian medicinal plants. Springer Science+Bussiness Media, New York; 2007. p. 426.
16. Kusuma SAF, Soraya RM, Indah F, Resmi M. Study on the antibacterial activity of fruit extracts of Klutuk Banana (Musa balbisiana colla) against Shigella dysenteriae ATCC 13313. Asian J Pharm Clin Res 2017;10:220-3.
17. Souza TP de, Jose Luiz GA, Ramon MP, Pedro RP. Development of granules from Phyllanthus nirurispray-dried extract. Braz J Pharm Sci 2009:45. http://dx.doi.org/10.1590/S1984-82502009000400009.
18. Crippa F. Problems of pharmaceutical technics with plant extracts. Fitoterapia 1978;49:257-63.
19. Bonati A. Problems relating to the preparation and use of extracts from medicinal plants. Fitoterapia 1980;1:5-12.
20. Soares LAL, George GO, Petrovick PR, Schmidt PC. Dry granulation and compression of spray-dried plant extracts. AAPS PharmSciTech 2005:6:E359-66.
21. Souza TP, Martinez PR, Gomez AJL, Petrovick PR. Eudragit E as an excipient for production of granules and tablets from phyllanthus niruri L spray-dried extract. AAPS PharmSciTech 2007;8:E1-E7.
22. Bauer KH, Fromming KH, Führer C. Pharmazeutische technologie, mit einführung in die biopharmazie. 9th ed. Wissenschaftliche Verlagsgesellschaft, Stuttgart; 2012.
23. Aulton ME. Pharmaceutics: the science of dosage forms design. Churchill Living Stone, London; 2002.
24. Nales, Diana A van Riet, Alfred FAM Schobben, Herman Vromans, Toine CG Egberts, Carin MA. Safe and effective pharmacotherapy in infants and preschool children: the importance of formulation aspects. Br Med J 2015;101:1-8.
25. Farnsworth NR. Biological and phytochemical screening of plant. J Pharm Sci 1966;55:243-69.
26. Palanisamy P, Abihishekh R, Kumar DY. Formulation and evaluation of instant tablets of acelofenac. Int J Res Pharm 2012;2:185-190.
27. Agoes G. Sediaan Farmasi Padat (SFI-6). Penerbit ITB Press, Bandung; 2012.
28. Depkes RI. Farmakope. 4th ed. Depkes, Jakarta; 1995.
29. Siregar CJP. Teknologi farmasi sediaan tablet dasar-dasar praktis. Penerbit EGC, Bandung; 2007. p. 275-80.
30. Irith W, Hilpert K, Robert EWH. Agar broth and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Prot 2008;3:163-5.
31. Kusuma SAF, Marlene A, Bobby EV. Formulation and evaluation of anti-acne gel containing citrus aurantifolia fruit juice using carbopol as a gelling agent. Int J Appl Pharm 2018;10:147-52.
32. Raimon. Perbandingan Metode Destruksi Basah dan Kering Secara Spektrofotometri Serapan Atom. Lokakarya Nasional Jaringan Kerjasama Kimia Analitik Indonesia. Yogyakarta; 1993.
33. Kumari S, Mazumder A, Bhattacharya S. Pharmacognostical and antimicrobial studies of the stem of Tabernaemontana divaricata Linn. Int J Pharm Pharm Sci 2015;7:101-4.
34. Juliantina FF, Citra DW, Nirwani B, Nurmasitoh T, Bowo T. Manfaat sirih merah (piper crocatum) sebagai agen anti bakterial terhadap bakteri gram positif dan gram negatif. JKKI, Jakarta; 2009.
35. Ahmad B, Mohd KS, Abdurrazak M, Rao M, Zin T. Phytochemical screening, the antioxidant activity of pure syringing in comparison to various solvents extracts of Musa paradisiaca (Banana) (Fruit and Flower) and total phenolic contents. Int J Pharm Pharm Sci 2015;7:242-7.
36. Arabski MS, Wasik K, Dworecki W, Kaca. Laser interferometric and cultivation methods for measurement of colistin/ampicillin and saponin interaction with smooth and rough of Proteus mirabilis lipopolysaccharides and cells. J Microb Methods 2009;77:179-83.
37. Karlina CY, Ibrahim M, Trimulyono G. Aktivitas antibakteri ekstrak herbal krokot (Portulaca oleracea L.) terhadap Staphylococcus aureus dan Escherichia coli. J UNESA Lentera Bio 2013;2:87-93.
38. Ajizah A. Sensitivitas Salmonella typhimurium terhadap Ekstrak Daun Psidium guajava L. Bioscientiae 2004;1:31-8.
39. Kawabata Y, Wada K, Nakatani M. Formulation design for poorly water-soluble drugs based on biopharmaceutics classification system: basic approaches and practical applications. Int J Pharm 2011;420:1-10.
40. Loftsson T, Brewster ME. Pharmaceutical applications of cyclodextrins. Drug solubilization and stabilization. J Pharm Sci 1996;85:1017-25.
41. Huiloh Z, Samanta AK, Heng PW. Overview of milling techniques for improving the solubility of poorly water-soluble drugs. Asian J Pharm Sci 2015;10:255-74.
42. Cho E, Jung S. Supramolecular complexation of carbohydrates for the bioavailability enhancement of poorly soluble drugs. Mol 2015;20:19620-46.
43. Chronakis, Ioannis S. On the molecular characteristics, compositional properties, and structural-functional mechanisms of maltodextrins: a review. Crit Rev Food Sci 2012;38:599-637.
44. Preis M, Miriam P, Breitkreutz J. Orodispersible film containing dimenhydrinate. Pharmaceutics 2012;4:551-62.
45. Lachman L, Lieberman HA, Kanig JL. Teori dan praktek farmasi industri. 2nd ed. UI Press, Jakarta; 1994.
46. Parrot EL. Pharmaceutical technology fundamental pharmaceutics. Burgess Publishing Company, Mineapolis; 1971.
47. Hu J, Johnston KP, Williams RO. Nanoparticle engineering processes for enhancing the dissolution rates of poorly water soluble drugs. Drug Dev Ind Pharm 2004;30:233-45.
48. Costa P, Sousa JM. Lobo modeling and comparison of dissolution profiles. Eur J Pharm Sci 2001;13:123-33.
49. Prakash K, Jieun R, Hyeongmin K, Iksoo K, Jeong TK, Hyunil K, et al. Pharmaceutical particle technologies: an approach to improve drug solubility, dissolution and bioavailability. Asian J Pharm Sci 2014;9:304-16.
50. Amidon GL, Lennernas H, Shah VP. A theoretical basis for a biopharmaceutic drug classification: the correlation of in vitro drug product dissolution and in vivo bioavailability. Pharm Res 1995;12:413-20.
51. Williams HD, Trevaskis NL, Charman SA. Strategies to address low drug solubility in discovery and development. Pharmacol Rev 2013;65:315-499.
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How to Cite
Kusuma, S. A., Mita, S., & Ermawati, R. (2018). EFFECT OF MALTODEXTRIN RATIO TO KLUTUK BANANA FRUIT EXTRACT (MUSA BALBISIANA COLLA) COMBINED WITH ITS PSEUDOSTEM EXTRACT ON ANTI-DYSENTERY GRANULE PERFORMANCE AND EFFECTIVITY. International Journal of Applied Pharmaceutics, 10(6), 187-193. https://doi.org/10.22159/ijap.2018v10i6.29305
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