THE EFFECT OF DIFFERENT STORAGE TEMPERATURES ON ANTISEPTIC GEL STABILITY CONTAINING GREEN TEA EXTRACT FORMULATED WITH ALOE VERA GEL
Keywords:Green tea, Aloe vera, Gel, Stability, Temperature, Influence
Objective: The aim of this study was to analyze the stability of antiseptic gel products containing green tea extract combined with aloe vera gel by observing the physical properties and the antibacterial effectivity during storage time with different temperature.
Methods: The gel of Aloe vera were separated from the leaves using a sterile knife. While the dried green tea leaves were extracted using a maceration method. Both of active agents were combined with different concentration and formulated into an antiseptic gel product. The carbopol in a fixed concentration was used as a gelling agent. The antiseptic gel in different formulas (F1-F9) were evaluated for 56 d in different temperature storage of 18 and 25 °C. The physical properties (color, pH and viscosity) were observed and the antibacterial effectivity of each formula was analyzed using the agar diffusion method against Staphylococcus aureus.
Results: As a result, the green tea extract and aloe vera gel were successfully incorporated into the carbopol formulation. Each formula represented a slight difference in the pH and viscosity value of each temperature during the storage time. But statistically, both temperatures did not give a significant difference to the pH and viscosity of each gel product. Therefore, the different effect given by the storage temperature was determined by analyzing the antibacterial effectivity results. The storage temperature of 18 °C gave a significant difference in the diameter of inhibition zones against S. aureus, compared to that of 25 °C. Formula number 9 containing the combination of 5% green tea extract and 6.24% aloe vera gel performed the highest antibacterial effectivity with the optimum storage temperature at 18 °C.
Conclusion: The storage temperatures used in this study gave significant effect to physical properties stability and its antibacterial effectivity.
Martın Diana AB, Rico D, Barry Ryan C. Green tea extract as a natural antioxidant to extend the shelf-life of fresh-cut lettuce. Innov Food Sci Emerg Technol 2008;9:593–603.
Boudreau MD, Beland FA. An evaluation of the biological and toxicological properties of aloe barbadensis (Miller), aloe vera. J Environ Sci Health 2006;24:103–54.
Jain S, Rathod N, Nagi R. Antibacterial effect of aloe vera gel against oral pathogens: an in vitro study. J Clin Diagn Res 2016;10:41-4.
Saks Y, Golan RB. Aloe vera gel activity against plant pathogenic fungi. Postharvest Biol Technol 1995;6:159-65.
Udgire MS, Pathade GR. Antibacterial activity of Aloe vera against skin pathogens. Am J Ethnomed 2014;1:147-51.
Taylor PW, Hamilton-Miller JM, Stapleton PD. Antimicrobial properties of green tea catechins. Food Sci Technol Bull 2005;2:71-81.
Gopal J, Muthu M, Paul D, Kim DH, Chun S. Bactericidal activity of green tea extracts: the importance of catechin containing nanoparticles. Sci Rep 2016;6:197-210.
Anjali S, Sonal G, Indira PS, Shweta D, Reema G. Greenxtract: possible mechanism and antibacterial activity on skin pathogens. Food Chem 2012;135:672-5.
Mitkari BV, Korde SA, Mahadik KR, Kokare CR. Formulation and evaluation of topical liposomal gel for fluconazole. Ind J Pharm Edu Res 2010;44:324–33.
Kaur LP, Guleri TK. Topical gel: a recent approach for novel drug delivery. Asian J Biomed Pharml Sci 2013;3:1-5.
Bhasha SA, Khalid SA, Duraivel S, Bhowmik D, Kumar KS. Recent trends in the usage of polymers in the formulation of dermatological gels. Indian J Res Pharm Biotech 2013;1:161–8.
Whitehouse MW. Anti-inflammatory glucocorticoid drugs: reflections after 60 y. Inflammopharmacology 2011;19:1–19.
Thakur L, Ghodasra U, Patel N, Dabhi M. Novel approaches for stability improvement in natural medicines. Pharmacogn Rev 2011;5:48-54.
Ramachandra CT, Srinivasa RP. Processing of aloe vera leaf gel: a review. Am J Agric Biol Sci 2008;3:502-10.
Leung AY. Aloe vera in cosmetics. Excelsa 1978;8:65-8.
Hamman JH. Composition and applications of aloe vera leaf gel. Mol 2008;13:1599-616.
McAnalley BH. Process for the preparation of aloe products. European Patent WO, 89/06539; 1993.
Gutterman Y, Chauser Volfson E. Changes in secondary phenolic metabolites during storage as an aqueous suspension in comparison with the content in harvested aloe arborescens leaves. Int J Food Sci Tech 2006;44:662-6.
Doriskumadoh, Ofori Kwakye K. Dosage forms of herbal medicinal products and their stability considerations-an overview. J Crit Rev 2017;4:1-8.
Fansworth NR. Biology and phytochemical screening of plants. J Pharm Sci 1966;55:263-4.
Pawar DP, Shamkuwar PB. Formulation and evaluation of herbal gel containing lantana camara leaves extract. Asian J Pharm Clin Res 2013;6:122-4.
Marlene A, Kusuma SAF. Comparison of thimerosal effectiveness in the formulation of eye drops containing neomycin sulfate and chloramphenicol. Int J Appl Pharm 2019;11:130-5.
Pandey A, Jagtap JV, Polshettiwar SA. Formulation and evaluation of in vitro antimicrobial activity of gel containing essential oils and effect of polymer on their antimicrobial activity. Int J Pharm Pharm Sci 2010;3:234-7.
Juliantina FF, Citra DW, Nirwani B, Nurmasitoh T, Bowo T. Manfaat sirih merah (piper crocatum) sebagai agen anti bakterial terhadap bakteri gram positif dan gram negatif. JKKI, Jakarta; 2009.
Arabski MS, Wasik K, Dworecki W, Kaca. Laser interferometric and cultivation methods for measurement of colistin/ampicillin and saponin interaction with smooth and rough of Proteus mirabilis lipopolysaccharides and cells. J Microb Methods 2009;77:179-83.
Tambekar DH, Dahikar SB. Antibacterial potential of some herbal preparation: alternative medicine in the treatment of enteric bacterial infection. Int J Pharm Pharm Sci 2010;2:176-9.
Esposito E, Carotta V, Scabbia A, Trombelli L, D'Antona P, Mene Gatti E, et al. Comparative analysis of tetracyclinecontaining dental gels: poloxamer and monoglyceride-based formulations. Int J Pharm 1996;142:9-23.
Fuente B, Igea A, Francisco OE, Mendez, Jos A. In vitro bioadhesion of carbopol hydrogels. Int J Pharm 1996;142:169-74.
Mohammad IT, Nair RH, Susan C, Chrisita A. Rheological characterization of topical carbomer gels neutralized to different pH. Pharm Res 2004;21:1192-9.
Dissemond J. Die bedeutung des pH-wertes fur die wunde. Hartmann Wundforum 2006;1:15–9.
Islam MT, Rodrıguez Hornedo N, Ciotti S, Ackermann C. Rheological characterization of topical carbomer gels neutralized to different pH. Pharm Res 2004;21:1192–9.