ANALYSIS OF SYNTHETIC RED DYES IN RED SPINACH SAMPLES (AMARANTHUS TRICOLOR L.)

  • Dolvin Novitasari Department of Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Rani Sauriasari Department of Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Maryati Kurniadi Department of Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.

Abstract

Objective: The purpose of this study was to determine whether synthetic red dyes are present in red spinach samples.
Methods: The presence of the dyes Ponceau 4R, Carmoisine, Rhodamine B, Amaranth was determined in red spinach using reactions, followed by
paper chromatography and thin-layer chromatography (TLC)-densitometry. In paper chromatography analyses, analytes were eluted using n-butanol–
ethanol–water (4:5:5) and isobutanol–ethanol–water (3:2:4), and in TLC-densitometry, analytes were eluted with n-butanol–ethanol–water (3:7:1).
Results: No synthetic red dyes were found in the seven red spinach samples.
Conclusion: The synthetic dyes Rhodamin B, amaranth, Ponceau 4R, and Karmoisin were not found as contaminants of red spinach.

Keywords: Amaranth, Karmoisin, Paper chromatography, Ponceau 4R, Red spinach, Rhodamin B, Thin-layer chromatography-densitometry, thinlayer chromatography.

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Fig. 1: Differences of pH and temperature between experiments performed on different days
The 2nd Physics and Technologies in Medicine and Dentistry Symposium (PTMDS), Universitas Indonesia. Depok, Indonesia 18
Int J App Pharm, Vol 10, Special Issue 1, 2018
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Novitasari, D., Sauriasari, R., & Kurniadi, M. (2018). ANALYSIS OF SYNTHETIC RED DYES IN RED SPINACH SAMPLES (AMARANTHUS TRICOLOR L.). International Journal of Applied Pharmaceutics, 10(1), 15-18. https://doi.org/10.22159/ijap.2018.v10s1.04
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