DEVELOPMENT OF A HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR ANALYZING DISODIUM 5â€²-GUANYLATE AND DISODIUM 5â€²-INOSINATE LEVELS IN FLAVOR ENHANCERS
Objective: This study aimed to develop a selective analytical method for assessing disodium 5â€²-guanylate and disodium 5â€²-inosinate levels in flavor
Methods: The levels were assessed using high-performance liquid chromatography (HPLC) with a photodiode array detector (PDA) (wavelength
=255 nm) and a SunFireÂ® C18 column (250 mm Ã— 4.6 mm Ã— 5 Î¼m). The mobile phase comprised a mixture of potassium phosphate buffer and an
ion pair reagent-hexane-1-sulfonic acid sodium salt - with a flow rate of 1.2 mL/min. The ion pair was used to generate a neutral equilibrium, which
resulted in increased retention of the analytes. Optimized analysis conditions were then validated regarding accuracy, precision, linearity, selectivity,
and the limits of detection and quantification.
Results: The average levels of disodium 5â€²-inosinate in the six analyzed samples were 0.24Â±1.46, 0.21Â±2.69, 0.58Â±3.26, 0.21Â±0.84, 0.22Â±3.59, and
0.47Â±2.21%, respectively. Regarding disodium 5â€²-guanylate, the average levels were 0.15Â±2.85, 0.15Â±0.12, 0.41Â±3.80, 0.16Â±1.72, 0.27Â±1.18, and
Conclusion: The optimal conditions for analyzing disodium 5â€²-guanylate and disodium 5â€²-inosinateusing HPLC with a PDA and SunFire C18 column
were Î»=255 nm, a mobile phase of potassium phosphate buffer and sodium hexane sulfonate, and a flow rate of 1.2 mL/min. For disodium 5â€²-inosinate,
its average levels in samples Aâ€“F were 0.24Â±1.46, 0.21Â±2.69, 0.58Â±3.26, 0.21Â±0.84, 0.22Â±3.59, and 0.47Â±2.21%, respectively. Meanwhile, the average
levels of disodium 5â€²-guanylate in the samples were 0.15Â±2.85, 0.15Â±0.12, 0.41Â±3.80, 0.16Â±1.72, 0.27Â±1.18, and 0.34Â±1.83%, respectively.
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