EFFECTS OF CURCUMIN AND NANOCURCUMIN ON CISPLATIN-INDUCED NEPHROTOXICITY IN RAT: COPPER TRANSPORTER 1 AND ORGANIC CATION TRANSPORTER 2 AS DRUG TRANSPORTERS

  • Deliana Nur Ihsani Rahmi Department of Biomedical Science Postgraduate Program, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia.
  • Melva Louisa Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia.
  • Vivian Soetikno Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia.

Abstract

Objective: This study aimed to investigate the efficacy of curcumin (CMN) and nanocurcumin (NC) at preventing cisplatin (CDPP)-induced
nephrotoxicity.
Methods: Two membrane transporters, copper transporter 1 (CTR1) and organic cation transporter 2 (OCT2), have been identified involved in active
accumulation of CDPP into renal tubular cells. We analyzed OCT2 transcription levels in rat kidney tissue and determined whether renoprotective
mechanism of CMN involves CTR1. Rats were randomly divided into five groups: (1) Control, (2) CDPP (7 mg/kg as single dose (i.p.), (3) CDPP+CMN
(7 mg/kg CDPP as a single dose, i.p.+100 mg/kg/day of CMN), (4) CDPP+50 mg NC (7 mg/kg CDPP as single dose, i.p.+50 mg/kg/day NC), and
(5) CDPP+100 mg NC (7 mg/kg CDPP as single dose, i.p.+100 mg/kg/day NC). Quantitative reverse transcription-polymerase chain reaction was
performed to calculate relative expression of CTR1 and OCT2 genes in rat kidney.
Results: Expression of CTR1 was unassociated with administration of CMN or NC, indicating CTR1 is uninvolved in renoprotective mechanism of CMN.
The administration of 100 mg/kg/day NC increased expression of OCT2; this increase was higher compared with normal expression levels. This may
be due to another regulatory mechanism from the CMN itself.
Conclusion: NC has a better renoprotective effect compared with curcumin, suggested by the increased OCT2 expression on its administration in
CDPP-treated rats.

Keywords: Cisplatin nephrotoxicity, Copper transporter 1, Curcumin, nanocurcumin, Organic cation transporter.

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Rahmi, D. N. I., Louisa, M., & Soetikno, V. (2018). EFFECTS OF CURCUMIN AND NANOCURCUMIN ON CISPLATIN-INDUCED NEPHROTOXICITY IN RAT: COPPER TRANSPORTER 1 AND ORGANIC CATION TRANSPORTER 2 AS DRUG TRANSPORTERS. International Journal of Applied Pharmaceutics, 10(1), 172-174. https://doi.org/10.22159/ijap.2018.v10s1.37
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