IN VITRO ANTIMALARIAL POTENCY OF ELEUTHERINE BULBOSA AND ITS EFFECT ON THE MORPHOLOGY OF PLASMODIUM FALCIPARUM

  • LUH SESOTYANING N Department of Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
  • MELVA LOUISA Department of Pharmacology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
  • HENDRI ASTUTY Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
  • DIN SYAFRUDDIN Malaria and Vector Resistance Unit, Eijkman Institute for Molecular Biology, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia.
  • PUJI BUDI SETIA ASIH Malaria and Vector Resistance Unit, Eijkman Institute for Molecular Biology, Dr. Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia.

Abstract

Objective: This study aimed to determine the potential inhibitory effects of Eleutherine bulbosa on the growth of Plasmodium falciparum and its toxic
effects on lymphocyte cells.
Methods: We performed a 50% inhibition concentration (IC50) test of E. bulbosa against P. falciparum, used transmission electron microscopy (TEM)
to observe Plasmodium morphology after exposure to E. bulbosa, and determined E. bulbosa’s cytotoxic concentration 50% (CC50) against lymphocytes.
Results: The IC50 value of E. bulbosa was 0.62 μg/ml. We observed crystals in the mitochondria of parasites under TEM. CC50 values at 24 h and 72 h
were 851.14 μg/ml and 707.94 μg/ml, respectively.
Conclusion: The main content of E. bulbosa is naphthoquinones that are suspected of having a mitochondrial target on the malaria parasite. E. bulbosa
is not toxic to lymphocytes; therefore, it has a potential as an antimalarial therapeutic.

Keywords: Eleutherine bulbosa, Antimalarial, Toxicity, 50% Inhibition concentration

References

1. Autino B, Corbett Y, Castelli F, Taramelli D. Pathogenesis of malaria in
tissues and blood. Mediterr J Hematol Infect Dis 2012;4:e2012061.
2. World Health Organization. WHO Global Malaria Programme. World
Malaria Report 2015. Available from: https: //www.apps.who.int/iris/
bitstream/handle/10665/200018/9789241565158_eng.pdf;jsessionid
= 5859DB1 FF50D46B863C585A70A6DCE13?sequence=1. [Last
accessed on 2016 Jan 28].
3. Indonesian Ministry of Health. Control of Drug and Insecticide
Resistance. Malaria Management Guidelines. 2014. p. 129-38.
4. Elyazar IR, Hay SI, Baird JK. Malaria distribution, prevalence, drug
resistance and control in Indonesia. Adv Parasitol 2011;74:41-175.
5. Kalauni SK, Awale S, Tezuka Y, Banskota AH, Linn TZ, Asih PB, et al.
Antimalarial activity of cassane-and norcassane-type diterpenes from
Caesalpinia crista and their structure-activity relationship. Biol Pharm
Bull 2006;29:1050-2.
6. Widyawaruyanti A, Zaini NC. Mechanism and antimalarial activities
of flavonoids compound isolated from cempedak (Artocarpus
champeden). J Bus Psychol 2011;13:67-77.
7. Mulia EP, Tantular IS, Mughni A. In vitro antimalarial activity of
belimbing wuluh (Averrhoa bilimbi) Leaves extract on Plasmodium
falciparum. Folia Med Indones 2012;48:96-101.
8. Rani VS, Nair BR. Pharmacognostic and physicochemical evaluation
of bulbs of Eleutherine bulbosa (Miller) urban, a medicinal plant. J
Pharmacogn Phytochem 2015;4:273-7.
9. Kuntorini EM, Nugroho LH. Structural development and bioactive
content of red bulb plant (Eleutherine americana); A traditional
medicines for local Kalimantan people. Biodiversitas 2010;11:102-6.
10. Amanda FR. Effectiveness of Dayak Onion Extract (Eleutherine
Palmifolia L.) Merr in Inhibiting Escherichia coli bacterial growth.
Undergraduate Thesis. Jakarta: Universitas Islam Negeri Syarif
Hidayatullah; 2014.
11. Nixon GL, Moss DM, Shone AE, Lalloo DG, Fisher N, O’Neil PM,
et al. Antimalarial pharmacology and therapeutics of atovaquone. J
Antimicrob Chemother 2013;68:977-85.
12. Pourahmad J, Salimi A. Isolated human peripheral blood mononuclear
cell (PBMC), a cost effective tool for predicting immunosuppressive
effects of drugs and xenobiotics. Iran J Pharm Res 2015;14:979-80.
13. Kovendan K, Murugan K, Panneerselvam C, Aarthi P, Kumar PM,
Subramaniam J, et al. Antimalarial activity of Carica papaya (Family:
Caricaceae) leaf extract against Plasmodium falciparum. Asian Pacific
J Trop Dis 2012;2:S306-11.
14. Winey M, Meehl JB, O’Toole ET, Giddings TH. Conventional
transmission electron microscopy. Mol Biol Cell 2014;25:319-22.
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How to Cite
N, L. S., LOUISA, M., ASTUTY, H., SYAFRUDDIN, D., & ASIH, P. B. S. (2019). IN VITRO ANTIMALARIAL POTENCY OF ELEUTHERINE BULBOSA AND ITS EFFECT ON THE MORPHOLOGY OF PLASMODIUM FALCIPARUM. International Journal of Applied Pharmaceutics, 11(1), 172-176. https://doi.org/10.22159/ijap.2019.v11s1.14
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Original Article(s)