ISOLATION, PURIFICATION, AND CHARACTERIZATION OF PORCINE SKIN COLLAGEN: ANALYSIS OF THE GLYCINE, PROLINE, AND HYDROXYPROLINE COMPONENTS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

  • Ayu Aditya Andayani Department of Pharmacy, Faculty of Pharmacy, University of Indonesia, Depok, 16424, Indonesia.
  • Harmita Harmita Department of Pharmacy, Faculty of Pharmacy, University of Indonesia, Depok, 16424, Indonesia.
  • Baitha Palanggatan Maggadani Department of Pharmacy, Faculty of Pharmacy, University of Indonesia, Depok, 16424, Indonesia.

Abstract

Objective: The aim of this study is to produce collagen through the extraction and isolation of porcine skin.
Methods: Collagen from porcine skin (Sus scrofa domesticus) was isolated, purified, and characterized. Major amino acid content of collagen (glycine,
proline, and hydroxyproline) was determined. Samples were extracted with 0.5 N acetic acid and precipitated with 0.9 M NaCl. Characterization tests
included those to determine the organoleptic content, pH, Fourier-transform infrared analysis, moisture content, ash content, viscosity, and Masson’s
trichrome staining on collagen tissue. The collagen was further analyzed using high-performance liquid chromatography using C-18® column and
a fluorescence detector at 265 nm and 320 nm, acetic buffer (pH 4.2)–acetonitrile (55:45) as mobile phase, and optimum flow rate of 0.8 mL/min.
Results: Our findings indicated that the best method for isolating collagen was with 0.1 M NaOH expressed by average contents of glycine, proline, and
hydroxyproline in collagen which were 33.663±0.215%, 12.333±0.128%, and 11.303±0.354%, respectively.
Conclusion: Porcine collagen has been successfully obtained with this method.

Keywords: Amino acid, Derivatization, Fluorescence, Glycine, High-performance liquid chromatography, Hydroxyproline, Porcine skin collagen, Praline.

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How to Cite
Andayani, A. A., Harmita, H., & Maggadani, B. P. (2018). ISOLATION, PURIFICATION, AND CHARACTERIZATION OF PORCINE SKIN COLLAGEN: ANALYSIS OF THE GLYCINE, PROLINE, AND HYDROXYPROLINE COMPONENTS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY. International Journal of Applied Pharmaceutics, 10(1), 294-298. https://doi.org/10.22159/ijap.2018.v10s1.65
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