QUANTIFICATION OF HYALURONIC ACID AND METHYLSULFONYLMETHANE IN DIETARY SUPPLEMENTS
Objective: Osteoarthritis can be treated by taking oral supplements containing compounds that can nourish bones and joints such as hyaluronic acid,
methylsulfonylmethane (MSM), chondroitin, glucosamine, and collagen. This study aimed to develop and validate tests for analyzing two compounds,
namely, hyaluronic acid and MSM, simultaneously and to determine both their levels in a mixed sample.
Methods: Hyaluronic acid derivatization was carried out using fluorenylmethyloxycarbonyl chloride and then analyzed by liquid chromatography
with fluorescence detection, while MSM was analyzed using gas chromatography. After the development of optimal conditions for each separation,
system suitability tests were developed and calibration curves used for tests of accuracy and precision as well as for level determination. Hyaluronic
was detected at an excitation wavelength of 255 nm and emission wavelength of 330 nm. The mobile phase used was acetonitrile-acetate pH 4.2 (1: 4)
with a flow rate of 1.0 mL/min.
Results: The developed method was linear (r=0.9983) in the range of 5–50 ppm and the limits of detection (LOD) and quantitation (LOQ) were 3.55
and 11.84 ppm, respectively. The initial column temperature for MSM analysis was 110°C and the mobile phase used was nitrogen gas at a flow rate of
0.8 mL/min. The method was linear (r=0.9998) in the range of 4000–15,000 ppm and the LOD and LOQ were 332.90 and 1109.67 ppm, respectively.
Conclusion: A simulated sample containing both compounds was assessed to contained 98.63% hyaluronic acid and 99.35% MSM.
Orive G, Hernández RM, Ciriza J, et al. Hyaluronic acid enhances
cell survival of encapsulated insulin-producing cells in alginate-based
microcapsules. Int J Pharm 2019;557:192-8.
2. Tokita Y, Okamoto A. Hydrolytic degradation of hyaluronic acid.
Polym Degrad Stab 1995;48:269-73.
3. Faust HJ, Sommerfeld SD, Rathod S, Rittenbach A, Ray Banerjee S,
Tsui BM, et al. A hyaluronic acid binding peptide-polymer system for
treating osteoarthritis. Biomaterials 2018;183:93-101.
4. Gocmen G, Gonul O, Oktay NS, Yarat A, Goker K. The antioxidant
and anti-inflammatory efficiency of hyaluronic acid after third molar
extraction. J Craniomaxillofac Surg 2015;43:1033-7.
5. Lin A, Nguy CH, Shic F, Ross BD. Accumulation of
methylsulfonylmethane in the human brain: Identification by
multinuclear magnetic resonance spectroscopy. Toxicol Lett
6. Basu, P, Sunny S, Maier C. Estrogenic and antiestrogenic activities
of commercial dietary supplements containing herbal ingredients and
isoflavones. Int J Pharm Pharm Sci 2016;8:307-12.
7. Volpi N. Hyaluronic acid and chondroitin sulfate unsaturated
disaccharides analysis by high-performance liquid chromatography
and fluorimetric detection with dansylhydrazine. Anal Biochem
8. Vatansever B, Binici B. A quantitative method for the measurement of
hydrolyzed type-I collagen protein in dietary supplement sirup using
HPLC-SEC-UV technique. J Chem Metrol 2015;1:1-15.
9. Podder AK. Qualitative and quantitative analysis of sildenafil in
This work is licensed under a Creative Commons Attribution 4.0 International License.