PHARMACOKINETIC PROFILE OF METFORMIN HYDROCHLORIDE IN DRIED BLOOD SPOT OF HEALTHY SUBJECTS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY–PHOTODIODE ARRAY

  • Yahdiana Harahap Bioavailability/Bioequivalence Laboratory, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.
  • Citra Dara Malia Bioavailability/Bioequivalence Laboratory, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.
  • Sunarsih . Bioavailability/Bioequivalence Laboratory, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.

Abstract

Objective: This study aimed to analyze the metformin hydrochloride in dried blood spot (DBS) sample and evaluate a pharmacokinetic profile in six
healthy subjects who administered 850-mg metformin hydrochloride in tablet using high-performance liquid chromatography–photodiode array
(HPLC–PDA).
Methods: Metformin analysis was performed on data of six healthy subjects who administered 850 mg of metformin hydrochloride as tablets. Blood
samples were taken at 12-time points at intervals of up to 12 h, and data were analyzed using HPLC–PDA.
Results: A linear calibration curve was obtained in the range of 25–5000 ng/mL, with r=0.9990. Pharmacokinetic profiles of metformin were obtained
using DBS samples from six healthy participants, with a Cmax of 347.3–416.22 ng/mL. The average tmax and t1/2 were obtained at 3 and 2 h, respectively,
and the area under the curve (AUC)0-t/AUC0-∞ ratio was >80% for all subjects.
Conclusion: The DBS biosampling technique can be developed for application in pharmacokinetic study of metformin HCl.

Keywords: Dried blood spot, High-performance liquid chromatography, Metformin hydrochloride, Pharmacokinetic profile, Validation.

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Harahap, Y., Malia, C. D., & ., S. (2018). PHARMACOKINETIC PROFILE OF METFORMIN HYDROCHLORIDE IN DRIED BLOOD SPOT OF HEALTHY SUBJECTS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY–PHOTODIODE ARRAY. International Journal of Applied Pharmaceutics, 10(1), 354-357. https://doi.org/10.22159/ijap.2018.v10s1.78
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