EFFECT OF 70% ETHANOL EXTRACT OF ORTHOSIPHONIS STAMINEUS BENTH LEAVES ON THE PHARMACOKINETIC PARAMETERS OF FUROSEMIDE IN MALE WHITE RATS


Riana Maya Oktaviani, Santi Purna Sari, Yahdiana Harahap

Abstract


Objective: This study aimed to observe the effect of the 70% ethanol extract of Orthosiphonis stamineus Benth leaves on the pharmacokinetic
parameters of furosemide in white male rats.
Methods: 18 Sprague–Dawley male rats were divided into three groups: The normal control group was given only 1% carboxymethyl cellulose,
the furosemide group was given 7.2 mg/200 g body weight (BW) suspension of furosemide, and the combination group was given 700 mg/kg BW
suspension of the 70% ethanolic extract of O. stamineus Benth leaves for 4 days followed by a 7.2 mg/200 g BW suspension of furosemide. On the
4th day of treatment, we performed orbital sinus blood sampling on the eyes of the rats and analyzed the levels of furosemide in plasma using highperformance
liquid chromatography.
Results: Therefore, the results showed that the administration of the 70% ethanol extract of O. stamineus Benth leaves improves the pharmacokinetic
parameters of furosemide on Cpmax and the area under the curve (p<0.05).
Conclusion: This study concludes that the 70% ethanol extract of O. stamineus Benth leaves improves the pharmacokinetic parameters of furosemide
in white male rats.


Keywords


Cat’s whiskers leaves, Furosemide, Orthosiphonis stamineus Benth, Pharmacokinetic, 70% ethanolic extract.

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References


Bragatto MS, Santos MB, Pinto AM, Gomes E, Angonese NT,

Viezzer WF, et al. Comparison between pharmacokinetic and

pharmacodynamic of single-doses of furosemide 40 mg tablets.

J Bioequiv Bioavailab 2011;48:519-32.

Şoica C, Vari C, Imre S, Gyéresi Á, Dehelean C, Dogaru M. HPLC

analysis of furosemide in rat plasma, bioavailability study. Farmacia

;56(5):513-20.

Muhtadi A, Wibowo MA, Hasanah AN, Muchtaridi M, Musfiroh I.

The effect of turmeric extract (Curcuma domestica Val.) on the

pharmacokinetic profile of paracetamol in male white Wistar rats. Res J

Pharm Biol Chem Sci 2015;6(6):406-14.

Badan Penelitian dan Pengembangan Kesehatan. Laporan Hasil

Riset Kesehatan Dasar (Riskesdas) Indonesia Tahun 2010. Jakarta:

Kementerian Kesehatan RI; 2010.

Bushra R, Aslam N, Khan AY. Food-drug interactions. Oman Med J

;26(2):77-83.

Welch WJ. Adenosine, Type 1 receptors: Role in proximal tubule Na

reabsorption. Acta Physiol (Oxf) 2015;213(1):242-8.

Jentzer JC, DeWald TA, Hernandez AF. Combination of loop diuretics

with thiazide-type diuretics in heart failure. J Am Coll Cardiol

;56(19):1527-34.

Departemen Kesehatan Republik Indonesia. Parameter Standar Umum

Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan RI; 2000.

Departemen Kesehatan Republik Indonesia. Materia Medika Indonesia.

Vol. 6. Jakarta: Departemen Kesehatan RI; 1995.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening

and extraction: A review. Int Pharm Sci 2011;1(1):98-106.

Sultana N, Arayne MS, Naveed S. RP-HPLC method for simultaneous

determination of Captopril and diuretics: Application in pharmaceutical

dosage forms and human serum. J Chromatogr Sep Tech 2011;2(2):1-4.

Shargel L, Wu-Pong S, Yu AB. Biofarmasetika dan Farmakokinetika

Terapan Edisi Kelima. Surabaya: Airlangga University Press; 2012.

Granero GE, Longhi MR, Mora MJ, Junginger HE, Midha KK,

Shah VP, et al. Biowaiver monographs for immediate release solid oral

dosage forms: Furosemide. J Pharm Sci 2010;99(6):2544-56.

Ameer OZ, Salman IM, Asmawi MZ, Ibraheem ZO, Yam MF.

Orthosiphon stamineus: Traditional uses, phytochemistry,

pharmacology, and toxicology. J Med Food 2012;15(8):678-90.

Yuliana ND, Khatib A, Link-Struensee AM, Ijzerman AP, Rungkat-

Zakaria F, Choi YH, et al. Adenosine A1 receptor binding activity

of methoxy flavonoids from Orthosiphon stamineus. Planta Med

;75(2):132-6.




About this article

Title

EFFECT OF 70% ETHANOL EXTRACT OF ORTHOSIPHONIS STAMINEUS BENTH LEAVES ON THE PHARMACOKINETIC PARAMETERS OF FUROSEMIDE IN MALE WHITE RATS

Keywords

Cat’s whiskers leaves, Furosemide, Orthosiphonis stamineus Benth, Pharmacokinetic, 70% ethanolic extract.

DOI

10.22159/ijap.2017.v9s1.28_33

Date

30-10-2017

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol. 9, Special Issue (Oct.), 2017 [PTMDS 2017] Page: 54-58

Online ISSN

0975-7058

Statistics

6 Views | 24 Downloads

Authors & Affiliations

Riana Maya Oktaviani
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia

Santi Purna Sari
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia

Yahdiana Harahap
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia


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