STUDY OF TYROSINASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL SCREENING OF CASSIA FISTULA L. LEAVES

  • Caroline Wijaya Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia
  • Berna Elya Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia
  • Arry Yanuar Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia

Abstract

Objective: This study was carried out to evaluate the phytochemical constituents and tyrosinase inhibitory activity of Cassia fistula leaves.
Methods: A tyrosinase inhibitory activity assay was performed by measuring the decrease in the intensity of color suggestive of the inhibition of
dopachrome formation resulting from the L-DOPA-tyrosinase reaction.
Results: The test results showed that the tyrosinase inhibitory activity of the water fraction of C. fistula leaf extract had the highest IC50 value
(152.031 μg/mL) among other fractions (n-hexane, ethyl acetate, and n-butanol). An enzyme kinetic assay showed that the water fraction of C. fistula
leaf extract inhibited tyrosinase with mixed-type inhibition. Phytochemical screening showed that the water fraction of C. fistula leaf extract contained
alkaloids, flavonoids, glycosides, phenols, and tannins.
Conclusion: The current study indicated that C. fistula leaves possess significant tyrosinase inhibitory activity.

Keywords: Cassia fistula, L-DOPA, Phytochemical screening, Tyrosinase inhibitor.

References

1. Chang TS. An updated review of tyrosinase inhibitors. Int J Mol Sci
2009;10:2440-75.
2. Kim YJ, Uyama H. Tyrosinase inhibitors from natural and synthetic
sources: Structure, inhibition mechanism and perspective for the future. Cell Mol Life Sci 2005;62:1707-23.
3. Smit N, Vicanova J, Pavel S. The hunt for natural skin whitening agents.
Int J Mol Sci 2009;10:5326-49.
4. Jothy SL, Zakaria Z, Chen Y, Lau YL, Latha LY, Sasidharan S, et al.
Acute oral toxicity of methanolic seed extract of Cassia fistula in mice.
Molecules 2011;16:5268-82.
5. Data of Cassia fistula L. 2012. Available from: http://www.
proseanet.org/prohati2/browser.php? docsid=137. [Last accessed on
21 Nov 2012].
6. Kaji NN, Khorana ML, Sanghavi MM. Studies on Cassia fistula Linn.
Indian J Pharm 1968;30:8-11.
7. Mahesh VK, Sharma R, Singh RS. Anthraquinones and kaempferol
from Cassia fistula species. J Nat Prod 1984;47:733-51.
8. Kuo YH, Lee PH, Wein YS. Four new compounds from the seeds of
Cassia fistula. J Nat Prod 2002;65:1165-7.
9. Narayanan V, Seshadri TR. Proanthocyanidins of Cassia fistula. Indian
J Chem 1972;10:379-81.
10. Kumar A, Pande CS, Kaul RK. Chemical examination of Cassia fistula
flowers. Indian J Chem 1966;4:460.
11. Senthil Kumar M, Sripriya R, Vijaya Raghavan H, Sehgal PK. Wound
healing potential of Cassia fistula on infected albino rat model. J Surg
Res 2006;131:283-9.
12. Bhakta T, Mukherjee PK, Saha K, Pal M, Saha BP, Mandal SC.
Evaluation of anti-inflammatory effects of Cassia fistula (Leguminosae)
leaf extract on rats. J Herbs Spices Med Plants 1999;6:67-72.
13. Luximon-Ramma A, Bahorun T, Soobrattee MA, Aruoma OI. Antioxidant
activities of phenolic, proanthocyanidin, and flavonoid components in
extracts of Cassia fistula. J Agric Food Chem 2002;50:5042-7.
14. Pradeep K, Mohan CV, Anand KG, Karthikeyan S. Effect of
pretreatment of Cassia fistula Linn. leaf extract against subacute CCl4
induced hepatotoxicity in rats. Indian J Exp Biol 2005;43:526-30.
15. Duraipandiyan V, Ignacimuthu S. Antibacterial and antifungal activity
of Cassia fistula L.: An ethnomedicinal plant. J Ethnopharmacol
2007;112:590-4.
16. Wagner H, Bladt S. Plant Drug Analysis: A Thin Layer Chromatography
Atlas. 2nd ed. Berlin, Germany: Springer; 1996.
17. Farnsworth NR. Biological and phytochemical screening of plants.
J Pharm Sci 1966;55:225-76.
18. Evans WC. Trease and Evans Pharmacognosy. 15th ed. London,
United Kingdom: WB Saunders; 2002.
19. Harborne JB. Phytochemicals Method: Plant Analysis Guidance in
Modern Way. Bandung: ITB Publisher; 1987.
20. Sumathy V, Lachumy SJ, Zakaria Z, Sasidharan S. In vitro
bioactivity and phytochemical screening of Musa acuminata flower.
Pharmacologyonline 2011;2:118-27.
21. ISO 14502-1: 2005. Determination of Substances Characteristic
of Green and Black Tea. Part 1: Content of Total Polyphenols in
Tea. Colorimetric Method using Folin-Ciocalteu Reagent. Geneva,
Switzerland: ISO; 2005.
22. Jackson FS, Barry TN, Lascano C, Palmer B. The extractable and
bound condensed tannin contents of leaves from tropical tree, shrub
and forage legumes. J Sci Food Agric 1996;71:103-10.
23. Santos-Buelga C, Williamson G. Methods in Polyphenol Analysis.
London, United Kingdom: Royal Society of Chemistry; 2003.
24. Lin MC, Tsai MJ, Wen KC. Supercritical fluid extraction of flavonoids
from scutellariae radix. J Chromatogr A 1999;830:387-95.
25. Escarpa A, Morales MD, Gonzales MC. Analytical performance of
commercially available and unavailable phenolic compounds using
real samples by high-performance liquid chromatography-diode-array
detection. Anal Chim Acta 2002;460:61-72.
26. Patel A, Zaveri M. Preliminary screening of some selected plants for
antityrosinase activity. Int J Inst Pharm Life Sci 2012;2:213-9.
27. Rao GV, Rao KS, Annamalai T, Radhakrishnan N, Mukhopadhyay T.
Chemical constituents and mushroom tyrosinase inhibition activity of
Chloroxylon swietenia leaves. Turk J Chem 2009;33:521-6.
28. Ramanathan K, Shanthi V, Kanika V. Exploration of plant bioactive
from Cassia fistula leaves for the treatment of ovarian cancer: An
integrative approach. Asian J Pharm Clin Res 2016;9:182-8.
29. Fidrianny I, Harnovi M, Insanu M. Evaluation of antioxidant activities
from various extracts of sweet orange peels using DDPH, frap assays
and correlation with phenolic, flavonoid, carotenoid content. Asian J
Pharm Clin Res 2014;7:186-90.
30. Rahman MH, Alam MB, Hossain MS, Jha MK, Islam A. Antioxidant,
analgesic and toxic potentiality of methanolic extract of Stephania
japonica (Thunb.) Miers. leaf. Asian J Pharm Clin Res 2011;4:38-41.
31. Batubara I, Darusman LK, Mitsunaga T, Rahminiwati M, Djauhari E.
Potency of Indonesian medicinal plants as tyrosinase inhibitor and
antioxidant agent. J Biol Sci 2010;10:138-44.
Statistics
65 Views | 73 Downloads
Citatons
How to Cite
Wijaya, C., Elya, B., & Yanuar, A. (2018). STUDY OF TYROSINASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL SCREENING OF CASSIA FISTULA L. LEAVES. International Journal of Applied Pharmaceutics, 10(1), 384-387. https://doi.org/10.22159/ijap.2018.v10s1.85
Section
Original Article(s)