• Nadya Febri Handayani Department of Phytochemical and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Berna Elya Department of Phytochemical and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Nuraini Puspitasari Department of Phytochemical and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.


Objective: The aim of this study was to test the anti-inflammatory activity of methanol, ethyl acetate, and n-hexane Cyclea barbata Mier. leaf extracts
using a lipoxygenase inhibition method.
Methods: Enzyme activity assay was performed using colorimetric method with lipoxygenase from soybean and linoleic acid as a substrate.
Absorbance was measured at 234 nm. The total flavonoid content of the most active extract was determined using a colorimetric method with AlCl3
and phytochemical screening.
Results: The ethyl acetate extract had the highest lipoxygenase inhibiting activity, with an IC50 value of 0.267 mg/mL. In addition, this extract had a
total flavonoid content of 21.62 mgQE/g and contained flavonoids, glycosides, and terpenoids.
Conclusion: This finding indicated that C. barbata provides a possible anti-inflammatory effect through the inhibition of lipoxygenase. However,
further research is still needed to strengthen this result.

Keywords: Anti-inflammation, Cyclea barbata Miers. leaves, Flavonoid content, Lipoxygenase, Phytochemical screening.


1. Kumar V, Abbas A, Aster J, Robbins S, Cornain S, Nasar I. Robbins
Pathology Textbook. Jakarta: Book Medical Publishers EGC; 2007.
2. Murray R, Granner D, Rodwell V. Biochemistry Harper. 27th ed. Jakarta:
EGC Book Medical Publishers; 2006.
4. Agric R. Benefits cincau cincau and manufacturing base in region Koto
Baru. ??? 2016;1:84-7.
5. Kusmardiyani S, Insanu M, Asyhar M. Effect of glycosidic flavonols
isolated from green grass jelly (Cyclea barbata Miers) leaves. Procedia
Chem 2014;13:194-7.
6. Farida YG. Toxicity test leaf extract and fraction in cincau black
(dazzling B. palustris) and cincau leaf green (Cyclea barbata L. Miers).
J Bahan Alam Indones 2012;2:1-6.
7. Kumar S, Pandey AK. Chemistry and biological activities of flavonoids:
An overview. ScientificWorldJournal 2013;2013:162750.
8. Deschamps JD, Kenyon VA, Holman TR. Baicalein is a potent in vitro
inhibitor against both reticulocyte 15-human and platelet 12-human
lipoxygenases. Bioorg Med Chem 2006;14:4295-301.
9. Choironi NA. Activity Inhibition of Lipoxygenase (LOX) by Tribe
Zingiberaceae Plant Extracts and Isolation of Selected Plant Secondary
Metabolites. Thesis of Pharmacy. Bandung Institute of Technology:
Unpublished; 2014.
10. Bhaigyabati T, Devi P, Bag G. Assessment of total flavonoids content
and antioxidant activity of aqueous extract of rhizome three Hedychium
of Averrhoa carambola L. leaves extract. Int J Chem Tech Res
12. Prakash H, Shailasree S, Sampath-Kumara K, Niranjana S. In vitro
antioxidant activity, lipoxygenase, cyclooxygenase-2 inhibition and
DNA protection properties of Memecylon species. Int J Pharm Pharm
Sci 2013;5:257-62.
13. Daughter N, Elya B, Puspitasari N. Antioxidant activity and
lipoxygenase inhibition test with total flavonoids content from Garcinia
kydia Roxburgh leaves extract. Pharmacog J 2017;9:280-4.
14. Puri D. Textbook of Medical Biochemistry. 3rd ed. India: Elsevier; 2011.
15. Bisswanger H. Enzyme assays. Perspect Sci 2014;1:41-55.
16. Kumazawa S, Suzuki S, Ahn M, Kamihira M, Udagawa Y, Bang K,
et al. A new chalcone from propolis collected on Jeju Island, Korea.
Food Sci Technol Res 2006;12:67-9.
17. Chedea VS, Jisaka M. Inhibition of soybean lipoxygenases-structural
and activity models for the lipoxygenase isoenzymes family. In: Recent
Trends for Enhancing the Diversity and Quality of Soybean Products.
Rijeka, Croatia: InTech; 2011. p. 113-5.
18. Paguigan N, Chichioco-Hernandez C. 15-Lipoxygenase inhibition of
selected Philippine medicinal plants. PHCOG J 2014;6:43-6.
19. Sauriasari R, Azizah N, Basah K. Tyrosinase inhibition, 2,2-diphenyl-
1-picrylhydrazyl radical scavenging activity, and phytochemical
screening of fractions and ethanol extract from leaves and stem bark of
matoa (Pometia pinnata). Asian J Pharm Clin Res 2017;10:85-9.
20. Elya B, Puspitasari N, Sudarmin AC. antioxidant activity and inhibition
of lipoxygenase activity ethanol extract of endosperm Arenga pinnata
(wurmb) Merr. Asian J Pharm Clin Res 2017;10:76-80.
21. Avelia S, Mauldina MG, Elya B. Antioxidant activity and lipoxygenase
inhibitory assay with total flavonoid content of Garcinia lateriflora
Blume leaves extract. Asian J Pharm Clin Res 2017;10:163-5.
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How to Cite
Handayani, N. F., Elya, B., & Puspitasari, N. (2018). CYCLEA BARBATA LEAF EXTRACT: LIPOXYGENASE INHIBITORY ACTIVITY AND PHYTOCHEMICAL SCREENING. International Journal of Applied Pharmaceutics, 10(1), 106-109.
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