ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS FROM ETHYL ACETATE AND METHANOL EXTRACTS OF GARCINIA DAEDALANTHERA PIERRE LEAVES
Objective: This study aimed to isolate and characterize the compounds responsible for the high antioxidant activities of the ethyl acetate and methanol
extracts of Garcinia daedalanthera Pierre leaves.
Methods: In this study, the ethyl acetate extract was obtained by column chromatography, and the methanol extract was obtained by vacuum column
chromatography. The mobile phase comprised n-hexane, ethyl acetate, and methanol with increased polarity. Antioxidant activity was examined using
the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The fraction with the highest antioxidant activity was purified through column chromatography,
recrystallization, and preparative thin-layer chromatography. This fraction, termed the isolate of B, was identified using DPPH and AlCl3, and its
antioxidant activity was quantitatively tested.
Results: From this research, 21.7 mg of the isolate of B were obtained with an IC50 of 5.82 Î¼g/mL. Identification using an AlCl3 sprayer produced
yellow phosphorescent spots under UV light. UV-Vis spectrum analysis revealed the presence of an aromatic compound and conjugated double bonds.
Infrared spectrum analysis revealed the presence of âˆ’OH, Câ€“H alkane, C=C aromatic, C=O, and C-O-C groups.
Conclusion: Based on this research, 21.7 mg of the isolate of B was derived through fractionation of the methanol extract, and this isolate exhibited
antioxidant activity with an IC50 of 5.82 Î¼g/mL. The isolate of B was considered to be a flavonoid, as it was fluorescent under UV light (366 nm) after
being sprayed with AlCl3 reagent.
Garcinia kola seeds. Food Chem Toxicol 2009;47:2620-3.
2. Hariyanto S. The diversity, distribution and potential of Garcinia
species in Indoensia. Berkala Penelitian Hayati 2007;12:129-35.
3. Sari R, Hanan A. Garcinia (Clusiaceae) at Bogor Botanical Gardens
Physiognomy, Diversity, and Potential. Bogor: Hari Cipta Puspa and
Satwa Kebun Raya Bogor Proceeding; 2000.
4. Elya B. Isolation and characterization of chemical compounds from
n-hexane extract of Garcinia rigida bark. J Makara Sains 2003;7:45-51.
5. Halliwell B. Handbook of Antioxidants. 2nd ed. Singapura: Taylor and
6. Fu M, Feng H, Chen Y, Wang D, Yang G. Antioxidant activity of
Garcinia xanthochymus leaf, root, and fruit extracts in vitro. Chin J Nat
7. Kothari S, Thompson A, Agarwal A, Du Plessis Stefan S. Free radicals:
Their beneficial and detrimental effects on sperm function. Indian J Exp
8. Erawati. Antioxidant Activity Test of Garcinia daedalanthera Pierre
Extract with DPPH Method and Identification of Chemical Compounds
from the Most Active. Thesis. Depok: Faculty of Pharmacy, Universitas
9. Mercy LS, Gnanamani A, Ilavarasan R. Physico-chemical,
phytochemical and high performance thin layer chromatography
analysis of the whole plant of Orthosiphon thymiflorus (roth.) sleesen.
Asian J Pharm Clin Res 2015;8:181-4.
10. Rohini RM, Amit KD. Determination of lupeol, B-sitosterol and
quercetin from ethyl acetate extract of Rhizophora Mucronata bark by
HPTLC technique. Asian J Pharm Clin Res 2011;4:103-5.
11. Ambarwati NS, Malik A, Deborah EA, Cakra HA, Hanif M, Elya B,
et al. The antibacterial activity of fractions of Ethyl acetate Garcinia
latissima miq. Stem bark extract against Bacillus subtilis and
Pseudomonas aeruginosa. Asian J Pharm Clin Res 2017;10:69-72.
12. Purwaningsih Y, Ersam T. The compound of xanthones as an antioxidant of
wood sticks Garcinia tetandra Pierre. Akta Kimia Indones 2007;2:103-8.
13. Margono SA, Zandrato RN. Synthesis of diasetil gamavuton-0 by
using acetyl chloride as acylating agent. Majalah Farmasi Indones
14. Supratman U. Elucidation of Structure of Organic Compounds,
Spectroscopic Method to Determine the Structure of Organic
Compounds. Jakarta: Widya Padjadjaran; 2010. p. 66-7.