• DEWI IRAWATI SOERIA SANTOSO Department of Medical Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • ADITYA K. MURTHI Department of Physiology, Faculty of Medicine, Trisakti University, Jakarta, Indonesia
  • SOPHIE YOLANDA Department of Medical Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia,
  • PATWA AMANI Graduate Student in Biomedical Sciences, Department of Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
  • IRENA UJIANTI Graduate Student in Biomedical Sciences, Department of Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
  • IMELDA ROSALYN SIANIPAR Department of Medical Physiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia,


Objective: Cobalamin deficiency may cause a lack of dietary methyl donors, which alter the heart metabolism. Cobalamin deficiency is common in patients with malnutrition, gastric ulcers, diabetes mellitus, and alcoholism. Most studies on cobalamin deficiency are focused on its relationship with oxidative stress and atherogenesis. Therefore, this study aims to find the correlation between cardiomyocyte’s energy metabolism in cobalamin deficiency and the risk of heart abnormalities through analysis of electrocardiography (ECG) patterns.

Methods: Adult male Sprague-Dawley rats (aged 24-28 w) were divided into 2 groups: the control group and cobalamin-deficient group. The control group was given standard diet while the treatment group received a modified diet, type AIN-93M (deficient in cobalamin), for a period of 16 w. ECG was performed in both groups on the last day of the 16-week period. Enzyme-linked immunosorbent assay (ELISA) test was also performed to evaluate plasma Hcy and B12 levels in each group at the end of the treatment period.

Results: At the end of the 16-week period, higher Hcy level and lower plasma B12 level were observed in the treatment group when compared to the control group. ECG patterns showed sinus rhythms in both groups, with a higher QRS amplitude and duration in the treatment group. Two of the seven rats in the treatment group developed cardiac arrhythmia.

Conclusions: Cobalamin deficiency impairs the heart’s energy metabolism with left ventricular enlargement and arrhythmia.

Keywords: Cobalamin deficiency, ECG abnormality, arrhythmia


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