EFFECT OF HIBISCUS SABDARIFFA LINN ON IL-6 AND TNF-Α LEVELS IN OVERTRAINED RAT HEART

  • DEWI IRAWATI SOERIA SANTOSO Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
  • SRI YUNITA Master Program in Biomedical Sciences, Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
  • NURUL PARAMITA Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
  • TRINOVITA ANDRAINI Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
  • NENG TINE KARTINAH Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia
  • GUSHAN FAHMI EL BAYANI Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia,
  • ERMITA I. IBRAHIM ILYAS Department of Medical Physiology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia

Abstract

Objective: This study aims to determine the effect of Hibiscus sabdariffa Linn. (HSL) administration on the interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) levels in rat heart. Overtraining was proved to increase the IL-6 and TNF-α levels in the blood, and HSL had anti-inflammatory and anti-oxidant properties. However, no studies have been conducted on the effect of methanolic extract of HSL administration on the IL-6 and TNF-α levels in overtrained rat heart.


Methods: This study used 25 male adult Wistar rats aged 8–10 w and weighing 200–250 g. The rats were randomly divided into five groups: control (C), control H. sabdariffa Linn (C+HSL), overtraining (OT), overtraining H. sabdariffa Linn (OT+HSL), and aerobic (A). Treatment was given 5 times a week for 11 w. At the end of the study, the IL-6 and TNF-α levels were measured using a standard ELISA kit.


Results: IL-6 and TNF-α levels in the heart were the highest in the overtraining group. The group that received HSL administration showed the lowest TNF-α and IL-6 levels. Conclusion: HSL could be a used to protect the heart from an inflammatory state, particularly in an overtraining condition.

Keywords: Overtraining, Interleukin-6, Tumor Necrosis Factor-alpha, Hibiscus sabdariffa Linn

References

1. Meeusen R, Duclos M, Foster C, Fry A, Gleeson M, Nieman D, et al. Prevention, diagnosis, and treatment of the overtraining syndrome: joint consensus statement of the European college of sport science and the American college of sports medicine. Med Sci Sports Exerc 2013;45:186–205.
2. Kreher J. Diagnosis and prevention of overtraining syndrome: an opinion on education strategies. Open Access J Sport Med 2016;7:115–22.
3. Kreher JB, Schwartz JB. Overtraining syndrome: a practical guide. Sports Health 2012;4:128–38.
4. da Rocha AL, Teixeira GR, Pinto AP, de Morais GP, Oliveira L da C, de Vicente LG, et al. Excessive training induces molecular signs of pathologic cardiac hypertrophy. J Cell Physiol 2018;233:8850–61.
5. Smith LL. Cytokine hypothesis of overtraining: a physiological adaptation to excessive stress? Med Sci Sports Exerc 2000;32:317–31.
6. Jones SA. Directing transition from innate to acquired immunity: defining a role for IL-6. J Immunol 2005;175:3463–8.
7. Fontes JA, Rose NR, Cihakova D. The varying faces of IL-6: from cardiac protection to cardiac failure. Cytokine 2015;74:62–8.
8. Bradham WS, Moe G, Wendt KA, Scott AA, Konig A, Romanova M, et al. TNF-? and myocardial matrix metalloproteinases in heart failure: relationship to LV remodeling. Am J Physiol Heart Circ Physiol 2002;282:H1288–95.
9. Sun M, Chen M, Dawood F, Zurawska U, Li JY, Parker T, et al. Tumor necrosis factor-? mediates cardiac remodeling and ventricular dysfunction after pressure overload state. Circulation 2007;115:1398–407.
10. Obouayeba AP, Meite S, Boyvin L, Yeo D, Kouakou TH, Guessan JDN. Cardioprotective and anti-inflammatory activities of a polyphenols enriched extract of Hibiscus sabdariffa petal extracts in Wistar rats. J Pharmacogn Phytochem 2015;4:57–63.
11. Fahmi G, Bayani EL, Marpaung NLE, Arnold D, Simorangkir S, Sianipar IR, et al. Anti-inflammatory effects of hibiscus sabdariffa linn. on the IL-1?/IL-1ra ratio in plasma and hippocampus of overtrained rats and correlation with spatial memory. Kobe J Med Sci 2018;64:73–83.
12. Ali MK, Ashraf A, Biswas NN, Karmakar UK, Afroz S. Antinociceptive, anti-inflammatory and antidiarrheal activities of ethanolic calyx extract of hibiscus sabdariffa Linn. (Malvaceae) in mice. J Chinese Integr Med 2011;9:626–31.
13. Da-Costa-Rocha I, Bonnlaender B, Sievers H, Pischel I, Heinrich M. Hibiscus sabdariffa L.-a phytochemical and pharmacological review. Food Chem 2014;165:424–43.
14. Hohl R, Ferraresso RLP, De Oliveira RB, Lucco R, Brenzikofer R, De Macedo DV. Development and characterization of an overtraining animal model. Med Sci Sports Exerc 2009;41:1155–63.
15. Lira FS, Rosa JC, Pimentel GD, Tarini VA, Arida RM, Faloppa F, et al. Inflammation and adipose tissue: effects of progressive load training in rats. Lipids Health Dis 2010;9:1–10.
16. Gholamnezhad Z, Hossein Boskabady M, Hosseini M, Sankian M, Khajavi Rad A, Rad KA. Evaluation of immune response after moderate and overtraining exercise in Wistar rat. Iran J Basic Med Sci 2014;17:1–8.
17. Dos Santos Cunha G, Ribeiro JL, De Oliveira AR. Overtraining: theories, diagnosis and markers. Rev Bras Med do Esporte 2006;12:267e–271e.
18. Giroir BP, Johnson JH, Brown T, Allen GL, Beutler B. The tissue distribution of tumor necrosis factor biosynthesis during endotoxemia. J Clin Invest 1992;90:693–8.
19. Sack MN. Tumor necrosis factor-a in cardiovascular biology and the potential role for anti-tumor necrosis factor-a therapy in heart disease. Pharmacol Ther 2002;94:123–35.
20. Hedayat M, Mahmoudi MJ, Rose NR, Rezaei N. Proinflammatory cytokines in heart failure: double-edged swords. Heart Fail Rev 2010;15:543–62.
21. Anda TK, Akahashi TT. Interleukin-6 and cardiovascular diseases. Japan Heart J 2003;45:183–93.
22. El-Menyar AA. Cytokines and myocardial dysfunction: state of the art. J Card Fail 2008;14:61–74.
23. Kao E, Hsu J, Wang C, Yang S, Lee H. Polyphenols extracted from hibiscus sabdariffa L. inhibited lipopolysaccharide-induced inflammation by improving antioxidative conditions and regulating cyclooxygenase-2 expression. Biosci Biotechnol Biochem 2014;73:385–90.
24. Liu J, Li X, Yue Y, Li J, He T, He Y. The inhibitory effect of quercetin on IL-6 production by LPS-stimulated neutrophils. Cell Mol Immunol 2005;2:455–60.
25. Nair M, Mahajan S, Reynolds J, Aalinkeel R, Nair H, Schwartz S, et al. The flavonoid quercetin inhibits proinflammatory cytokine (tumor necrosis factor alpha) gene expression in normal peripheral blood mononuclear cells via modulation of the NF-? ? system. Clin Vaccine Immunol 2006;13:319–28.
26. Hartati FK, Widjanarko SB, Widyaningsih TD, Rifai M. Anti-inflammatory evaluation of black rice extract inhibits TNF-?, IFN-? and IL-6 cytokines produced by immunocompetent cells. Food Agric Immunol 2017;28:1116–25.
27. Kim JN, Han SN, Ha TJ, Kim HK. Black soybean anthocyanins attenuate inflammatory responses by suppressing reactive oxygen species production and mitogen activated protein kinases signaling in lipopolysaccharide-stimulated macrophages. Nutr Res Pract 2017;11:357–64.
28. Kim HJ, Xu Lianji, Chang KC, Shin SC, Chung JI, Kang Dawon, et al. Anti-inflammatory effects of anthocyanins from black soybean seed coat on the keratinocytes and ischemia reperfusion injury in rat skin flaps. Microsurgery 2012;32:563–70.
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SANTOSO, D. I. S., YUNITA, S., PARAMITA, N., ANDRAINI, T., KARTINAH, N. T., BAYANI, G. F. E., & ILYAS, E. I. I. (2019). EFFECT OF HIBISCUS SABDARIFFA LINN ON IL-6 AND TNF-Α LEVELS IN OVERTRAINED RAT HEART. International Journal of Applied Pharmaceutics, 11(6). https://doi.org/10.22159/ijap.2019.v11s6.33535
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