MECHANISM OF SOLANUM BETACEUM TO PREVENT MEMORY IMPAIRMENT IN CIGARETTE SMOKE-EXPOSED RAT
Objective: The aim of this study was conducted to evaluate the neuroprotective role of Solanum betaceum against memory impairment due to chronic cigarette smoke exposure in rat brain.
Methods: Adult male albino rats were exposed to cigarette smoke for 28 days, 3 pc cigarette/day, and simultaneously administered with S. betaceum in Groups K2, K3, and K4 (100 mg/kg b.w/day, 200 mg/kg b.w/day, and 400 mg/kg b.w/day), respectively. The level of N-methyl-D-aspartate (NMDA), c-AMP response element binding (CREB) protein, brain-derived neurotrophic factor (BDNF), number of neuron and glial cells, and memory was also measured.
Results: S. betaceum administration could prevent from memory impairment significantly (p<0.05) by decreased time to reach the target at Y-Maze and maintained the levels of CREB, BDNF, neuron, and glial cells (microglia, astrocytes, and oligodendrocytes) significantly (p<0.05) but did not significantly decreased NMDA levels (p>0.05).
Conclusion: Exposure to cigarette smoke compromised the memory functions. The result of this study shown that administration of S.betaceum could inhibit memory impairment and inhibit the decrease of neuron cells , increase the level of BDNF and number of glia cells including microglia, astrocytes and oligodendrocytes. The mechanism of S. betaceum to prevent memory impairment through activation of CREB (the transcription factor) which further enhances the formation of BDNF (the neurotrophic factors), thus increase activation of the glia cells to protect brain cell damage, thus preventing memory impairment due to cigarette smoke exposure.
2. Susanto Y, Djojosoewarno P, Rosnaeni R. Effect of light exercise towards short term memory in adult women. J Knowl Manag 2009;8:144-50.
3. Ardianingsih F. Efek Pemberian Jus Bayam Merah (Amaranthus tricolor Linn.) terhadap Memori Mencit (Mus musculus) yang Diinduksi Timbal Asetat. Thesis Thesis, Universitas Airlangga; 2012.
4. Durazzo TC, Meyerhoff DJ, Nixon SJ. Chronic cigarette smoking: Implications for neurocognition and brain neurobiology. Int J Environ Res Public Health 2010;7:3760-90.
5. Mazzone P, Tierney W, Hossain M, Puvenna V, Janigro D, Cucullo L. Pathophysiological impact of cigarette smoke exposure on the cerebrovascular system with a focus on the blood-brain barrier: Expanding the awareness of smoking toxicity in an underappreciated area. Int J Environ Res Public Health 2010;7:4111-26.
6. Omotoso GO, Babalola FA. Histological changes in the cerebelli of adult wistar rats exposed to cigarette smoke. Niger J Physiol Sci 2014;29:43-6.
7. Adeniyi PA, Ogundele OM. Smoke and ethanolic extract of Nicotiana tabacum altered hippocampus histology and behaviour in mice. J Cell Anim Biol 2014;8:34-40.
8. Adeniyi PA, Musa AA. Comparative effects of smoke and ethanolic extract of Nicotiana tabacum on hippocampus and neurobehaviour of mice. Res Pharm Biotechnol 2011;3:68-74.
9. Chen R, Wilson K, Chen Y, Zhang D, Qin X, He M, et al. Association between environmental tobacco smoke exposure and dementia syndromes. BMJ J 2013;70:63-9.
10. Barnes DE, Haight TJ, Mehta KM, Carlson MC, Kuller LH, Tager IB. Secondhand smoke, vascular disease, and dementia incidence: Findings from the cardiovascular health cognition study. Am J Epidemiol 2010;171:292-302.
11. Lister CE, Morrison SC, Kerkhofs NS, Wright KM. The nutritional composition and health benefits of New Zealand tamarillos. Crop Food Res Confid Rep 2005;2005:1-17.
12. Kumalaningsih S. Antioksidan Alami Terong Belanda (Tamarillo). Indonesia: Trubus Agrisarana; 2006. p. 4-11.
13. Kujawski R, Kujawska M, Marcin O, Baraniak J, Sobczak A. Perspectives for gallotannins neuroprotective potential-current experimental evidences. J Med Sci 2016;85:313-8.
14. Yoo KY, Park SY. Terpenoids as potential anti-alzheimer’s disease therapeutics. Molecules 2012;17:3524-38.
15. Spencer JP. Flavonoids and brain health: Multiple effects underpinned by common mechanisms. Genes Nutr 2009;4:243-50.
16. Rendeiro C, Rhodes JS, Spencer JP. The mechanisms of action of flavonoids in the brain: Direct versus indirect effects. Neurochem Int 2015;89:126-39.
Fig. 3: Path analysis of direct effect Solanum betaceum to prevent memory impairment due to cigarette smoke exposure. *=significant with p<0.05, ? value shows positive or negative correlation coefficient
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17. Kandimalla R, Kalita S, Choudhury B, Kotoky J. Review article a review on anti-diabetic potential of genus Solanum (Solanaceae). J Drug Deliv Ther 2015;5:24-7.
18. Nowinski WL. Introduction to brain anatomy. In: Miller K, editor. Biomechanics of the Brain, Biological and Medical Physics, Biomedical Engineering. New York: Springer; 2011. p. 5-40.
19. Ernst M, Matochik JA, Heishman SJ, Van Horn JD, Jons PH, Henningfield JE, et al. Effect of nicotine on brain activation during performance of a working memory task. Proc Natl Acad Sci U S A 2001;98:4728-33.
20. Hu YS, Long N, Pigino G, Brady ST, Lazarov O. Molecular mechanisms of environmental enrichment: Impairments in akt/GSK3?, neurotrophin-3 and CREB signaling. PLoS One 2013;8:e64460.
21. Johnston GA. Flavonoid nutraceuticals and ionotropic receptors for the inhibitory neurotransmitter GABA. Neurochem Int 2015;89:120-5.
22. Silva AR, Pinheiro AM, Souza CS, Freitas SR, Vasconcellos V, Freire SM, et al. The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures. Cell Biol Toxicol 2008;24:75-86.
23. Suk K, Lee H, Kang SS, Cho GJ, Choi WS. Flavonoid baicalein attenuates activation-induced cell death of brain microglia. J Pharmacol Exp Ther 2003;305:638-45.
24. Diniz TC, Silva JC, de Lima-Saraiva SR, Ribeiro FP, Pacheco AG, de Freitas RM, et al. The role of flavonoids on oxidative stress in epilepsy. Oxid Med Cell Longev 2015;2015:171756.
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