• Robert Tungadi State University of Gorontalo


Objective: Nanoparticle preparation can increase the bioavailability of anchovy protein. This study aims to optimize and characterize anchovy protein concentrate nanoparticle based on volume comparison of chitosan-TPP (Tripolyphosphate) matrix in forming the size of anchovy protein concentrate nanoparticle with ionic gelation method.

Methods: Fresh anchovy was extracted by acid base method using solvents of NaOH and HCl to get protein concentrate. After that, it was conducted by formulating anchovy protein concentrate into a mixture of chitosan-TPP matrix with volume ratio which was divided into 5 series of variations, namely F1 without matrix, F2 1:1, F3 1:3, F4 1:5 and F5 1:7 ratio of chitosan to TPP, then formed matrix was determined the value of the encapsulation efficiency and characterized using a particle size analysis tool.

Results: The results showed that the optimum volume comparison of the chitosan-TPP matrix in forming nanoparticle of anchovy protein concentrate was ratio 1:7 because this has a matrix encapsulation efficiency value of 99.11%, a small particle concentrate size of 687.26 nm, a good polydispersity index value of 0.406 and a value of zeta potential of +12.9 mV.

Conclusion: The optimum formula of chitosan-TPP matrix volume ratio in forming anchovy protein concentrate nanoparticle is F5 with the ratio of chitosan-TPP matrix mixture 1:7.

Keywords: Anchovy, protein, Nanoparticle, Ioninc Gelation, Chitosan-TPP

Author Biography

Robert Tungadi, State University of Gorontalo
Department of Pharmacy


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How to Cite
Tungadi, R. (2020). PREPARATION AND CHARACTERIZATION OF ANCHOVY (Stolephorus sp) PROTEIN CONCENTRATE NANOPARTICLE USING IONIC GELATION METHOD. International Journal of Applied Pharmaceutics, 12(2). Retrieved from
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