FORMULATION AND CHARACTERIZATION OF SELF NANO-EMULSIFYING DRUG DELIVERY SYSTEM (SNEDDS) FRACTION OF N-HEXANE: ETHYL ACETATE FROM SESEWANUA LEAF (CLERODENDRUM FRAGRANS WILD.)
Keywords:surfactant, co-surfactant, particle size, polydispersity index
Objective: Sesewanua leaves contain alkaloid compounds as antioxidants, and its leaves can be used to formulate SNEDDS dosage forms, which can effectively deliver the medicine.
This study intended to determine the variation of surfactant concentration (Tween 80) and cosurfactant (PEG 400) towards pH, viscosity, nano-emulsion duration and characterization using PSA method (particle size and polydispersity index).
Methods: This study employed quasi-experimental method and the independent variables in this study were variations in the concentration of surfactant (Tween 80) and cosurfactant (PEG 400), which consist of 3 formulas, such as SFS 1 (6:3), SFS 2 (7:2), and SFS 3 (8:1). The dependent variables in this study including pH, viscosity, nano-emulsion time, particle size and polydispersity index which utilized One Way Anova Post Hoc LSD (p>0.05) and Tamhane (p<0.05) tests as the data analysis.
Results: The pH test SFS1-SFS3 has a pH value of 7.92, 8.30 and 8.35, followed by Viscosity test SFS1-SFS3 which has a viscosity value of 1.00 cP, 1.38 cP and 2.91 cP. Further, SFS1-SFS3 nano emulsified time test had nano emulsified time in gastric and intestinal fluids 35.18s & 43.96s, 43.54s & 47.13s and 44.00s & 50.29s. Characterization of SFS1-SFS3 particle size in gastric and intestinal fluids 23.9nm & 23.0nm, 18.5nm & 22.7nm and 19.1nm & 22.9nm, while characterization of SFS1-SFS3 polydispersity index in gastric and intestinal fluids were 0.433 & 0.348, 0.451 & 0.440 and 0.568 & 0.462.
Conclusion: The increase of variations in surfactant concentration and decreased cosurfactant significantly affected pH, viscosity, nano-emulsion time, and particle size of SFS preparations. However, the polydispersity index was not considerably affected.
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Copyright (c) 2022 ZULFIAYU SAPIUN, ARLAN K. IMRAN, SISILIA TERESIA ROSMALA DEWI, DHEA FADILA MASITA PADE, WIDYAWATI IBRAHIM, ROBERT TUNGADI, WIDY SUSANTI ABDULKADIR, YOS BANNE, YUSNITA RIFAI, SARTINI SARTINI, ANDI DIAN PERMANA, YUSNITA RIFAI, YSRAFIL YSRAFIL, NANGSIH SULASTRI SLAMET
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