DEVELOPMENT OF EFFERVESCENT GRANULE OF CORN MILK, SUPPLEMENTED WITH PROBIOTICS LACTOBACILLUS STRAIN SHIROTA
Objective: One of the materials which can be formulated into probiotics drink is corn milk. It has nutrient contents such as vitamin A, B, C, minerals, and fibres. The objective of this study was to develop and evaluate the probiotic drink of corn milk which was formulated into an effervescent granule.
Methods: The first step was carried out to make corn extract with the addition of Lactobacillus strain Shirota and incubated 24 h at 40 Â°C to gain corn milk. After that, corn milk was centrifuged to get filtrate and dried by oven 40 Â°C for 2 d to gain dry powder. Then corn milk powder was formulated into effervescent granule (3 formulas) using different ratio of acids. The method which used in making effervescent granule of corn milk was wet granulation. All formulas were done evaluations including physical and chemical properties of granule and hedonic test.
Results: The results showed that formula 1 (only citric acid 23.84%) gave unsatisfactory results particularly on the evaluation tests such as LOD 9.64%, dissolution time 3.25 min, moisture content 10.67% and hedonic test which did not meet the requirements. Otherwise, formula 2 (only tartaric acid 23.84%) and formula 3 (citric acid 7.94% and tartaric acid 15.9% combination) showed satisfactory results for all evaluation tests of granules. Particularly formula 3 gave pH 5 on pH test which was the same as pH lactic acid for fermentation and LOD 4.34%, MC 4.53%, Ïb 0.53 g/ml, Ït 0.62 g/ml and hedonic test around 80%.
Conclusion: Formula 3 was the best formulation based on the evaluation and the stability of corn milk effervescent granule.
2. Supavititpatana P, Wirjantoro TI, Raviyan P. Characteristics and shelf-life of corn milk yogurt. CMU J Nat Sci 2010;9:133-47.
3. Yasni S, Maulydia A. Development of corn milk yoghurt using the mixed culture of Lactobacillus delbruekii, Streptococcus salivarus, and Lactobacillus casei. Hayati J Biosci 2014;21:1-7.
4. Ansel HC, Popovich NG, Allen LV. Pharmaceutical dosage forms and drug delivery systems. B. I. Waverly, New Delhi; 1999. p. 469-71.
5. Rukmana R. The corn production in Indonesia. Publisher Aneka Ilmu. Semarang; 2003.
6. Lieberman HA, Lachman L, SchwartzJ B. Pharmaceutical dosage forms: tablets, Marcel Dekker inc. Vol. 1-2, New York; 1993. p. 285.
7. Gupta R, Sharma P, Garg A, Soni A, Sahu A, Rai S, et al. Formulation and evaluation of herbal effervescent granules incorporated with Calliandra Haematocephala leaves extract. Indo Am J Pharm Res 2013;3:4366-71.
8. Nagashima AI, Pansiera PE, Baracat MM, GÃ³mez RJH. Development of effervescent products, in powder and tablet form, supplemented with probiotics Lactobacillus acidophilus and Saccharomyces boulardii. Food Sci Technol Campinas 2013;33:605-11.
9. Muhajir R, Rahim A, Hutomo GS. The physical and chemical characteristics of sweet corn milk at a different boiling time. J Agroland 2014;21:95-103.
10. Anshory H, Syukuri Y, Malasari Y. The formulation of an effervescent tablet of ginseng jawa (Tlinumpaniculatum) extracts with aspartam variations. J Sci Pharm 2007;4:1-7.
11. Syamsul ES, Supomo. Formulation of effervescent powder of water extract of bawang tiwai (EleuterinePalmifolia) as a healthy drink. Trad Med J 2014;19:113-7.
12. Mohrle R. Effervescent tablets. In: lieberman HA, Lachman L. eds. Vol. I. Pharmaceutical Dosage Form Tablet; 1989. p. 287, 289, 295.
13. Limyati VY. The formulation of effervescent powder of carrot extract (Daucuscarota L). Thesis. Faculty of Agriculture Technology. Padang: Andalas University; 2009.
14. Lee WJ, Lucey JA. Formation and physical properties of yoghurt. Asian-Aust J Anim Sci 2010;23:1127-36.
15. Cobbeldick RH. High sugar sweet corn. Ontario: Factsheet, Ontario Department of Agriculture; 2012.
16. Aswal P, Priyadarsi S, Anubha S. Yoghurt preparation, characteristic and recent advancements. Cibtech J Bioprotocols 2012;2:2319-40.
17. Fernandez Garcia E, Vilaviciosa C, McGregor JU. Determination of organic acid during fermentation and cold storage of yoghurt. J Dairy Sci 1994;77:2934-9.
18. Tamime AY, Robinson RK. Yoghurt science and technology. New York: Pergamon Press Ltd; 1989.
19. Gilliland SE, Reilly SS, Kim GB, Kim HS. Viability during storage of selected probiotic lactobacilli and bifidobacteria in the yoghurt-like product. J Food Sci 2002;67:3091-5.
20. Velivela S, Abbulu K, Vima M, Pati NB. Formulation and in vitro evaluation of ritonavir floating tablets by melt granulation technique. Int J Appl Pharm 2016;8:12-5.