FORMULATION OF SERUM GEL CONTAINING ANGELICA KEISKEI LEAF EXTRACT AS AN ANTIOXIDANT AND TYROSINASE ENZYME INHIBITOR

  • DIAH LIA AULIFA Department of Pharmaceutical Biology, Indonesian School of Pharmacy, JI Soekarno Hatta no.354, Bandung, Indonesia
  • RIZKA Y. NOERFITRI Department of Pharmaceutical Biology, Indonesian School of Pharmacy, JI Soekarno Hatta no.354, Bandung, Indonesia
  • DEBY TRISTIYANTI Department of Pharmaceutical Technology, Indonesian School of Pharmacy, JI Soekarno Hatta no.354, Bandung, Indonesia
  • ARIF BUDIMAN Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy Universitas Padjadjaran, Indonesia

Abstract

Objective: The aim of this study was to formulate and evaluate the efficacy of Angelica keiskei (AK) leaf extract in serum gel as an antioxidant and tyrosinase enzyme inhibitor.


Methods: The leaves of AK were extracted using the maceration method, while the antioxidant and tyrosinase enzyme inhibitory activities were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and L-Tyrosine as assay and substrate, respectively. Subsequently, the extract was formulated into serum gel and evaluated for physical stability, antioxidant and inhibitory activity to tyrosinase enzyme.


Results: The results showed that AK leaf extract has antioxidant activity, with an IC50 value = 7.73 mg/ml, while the tyrosinase enzyme inhibitory power had an IC50 value = 500 mg/ml. Based on physical stability, the serum gel was stable after 28 d of storage. The serum gel of AK leaf extract also showed antioxidant activity and tyrosinase enzyme inhibition with IC50 value of 16.68 mg/ml and 741.62 mg/ml, respectively.


Conclusion: This study demonstrated that the serum gel of AK leaf extract has potential as a skin lightening agent and is safe for use as a topical preparation.

Keywords: Angelica keiskei extract, Serum gel, Antioxidant, Tyrosinase enzyme inhibitor

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AULIFA, D. L., NOERFITRI, R. Y., TRISTIYANTI, D., & BUDIMAN, A. (2020). FORMULATION OF SERUM GEL CONTAINING ANGELICA KEISKEI LEAF EXTRACT AS AN ANTIOXIDANT AND TYROSINASE ENZYME INHIBITOR. International Journal of Applied Pharmaceutics, 12(3), 108-111. https://doi.org/10.22159/ijap.2020v12i3.37303
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