FORMULATION OF EFFERVESCENT GRANULES FROM RED GINGER (ZINGIBERIS OFFICINALE ROSCOE VAR. RUBRUM) EXTRACT AND ITS ANTIOXIDANT ACTIVITY

Authors

  • DIAH LIA AULIFA Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran
  • DIKI PRAYUGO WIBOWO Department of Pharmaceutical Biology, Indonesian School of Pharmacy (Sekolah Tinggi Farmasi Indonesia), JI Soekarno Hatta no.354, Bandung, Indonesia https://orcid.org/0000-0002-2659-8288
  • NENI SAFITRI Department of Pharmaceutical Biology, Indonesian School of Pharmacy (Sekolah Tinggi Farmasi Indonesia), JI Soekarno Hatta no.354, Bandung, Indonesia
  • ARIF BUDIMAN Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran

DOI:

https://doi.org/10.22159/ijap.2022v14i1.43377

Keywords:

Red ginger, Effervescent granules, Antioxidant, Flowability

Abstract

Objective: Ginger is one of the Indonesian plants that has been used as traditional medicine. The flavonoids and phenols compounds contained high antioxidant activity. This study aimed to formulate effervescent granules (EG) from red ginger (RG) extract and evaluate its antioxidant activity.

Methods: The formulation of EG from RG extract was prepared by the wet granulation method using different concentrations of polyvinylpyrrolidone (PVP). Furthermore, the flowability of granules was evaluated, including flow rate, angle of repose, bulk density, tapped density, Carr's index, Hausner ratio, and effervescent time. The physical stability of granules such as organoleptic evaluation, effervescent time, and pH measurement was also evaluated after 28 d of storage, and the antioxidant activity of EG from RG extract was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH).

Results: The result showed that the EG of RG extract was successfully prepared by wet granulation with a concentration of 15%. In addition, the flowability study showed that all formulas of EG from RG extract have good flow properties, and the granules showed excellent flow properties based on Carr′s index results. The effervescent time of granules remained within the acceptable range according to USP, and the physical stability did not change even after 28 d of storage. The IC50 of EG from RG extract was 283.28±3.6 ppm and has moderate in free radicals scavenging activity.

Conclusion: EG from RG extract can be used as food supplements to protect the human body from free radicals and inhibit oxidases.

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References

Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature. 2000;408(6809):239-47. doi: 10.1038/35041687, PMID 11089981.

Halliwell B, Gutteridge JM. Free radicals in biology and medicine. Oxford: Oxford University Press; 2015.

Oboh G, Akinyemi AJ, Ademiluyi AO. Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe(2+) induced lipid peroxidation in rat brain in vitro. Exp Toxicol Pathol. 2012;64(1-2):31-6. doi: 10.1016/j.etp.2010.06.002, PMID 20598871.

Patel RP, Moellering D, Murphy Ullrich J, Jo H, Beckman JS, Darley Usmar VM. Cell signaling by reactive nitrogen and oxygen species in atherosclerosis. Free Radic Biol Med. 2000;28(12):1780-94. doi: 10.1016/s0891-5849(00)00235-5, PMID 10946220.

Yeh HY, Chuang CH, Chen HC, Wan CJ, Chen TL, Lin LY. Bioactive components analysis of two various gingers (Zingiber officinale Roscoe) and antioxidant effect of ginger extracts. LWT Food Sci Technol. 2014;55(1):329-34. doi: 10.1016/j.lwt.2013.08.003.

Ibanez E, Kubatova A, Senorans FJ, Cavero S, Reglero G, Hawthorne SB. Subcritical water extraction of antioxidant compounds from rosemary plants. J Agric Food Chem. 2003;51(2):375-82. doi: 10.1021/jf025878j, PMID 12517098.

Ghasemzadeh A, Jaafar HZ, Rahmat A. Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules. 2010;15(6):4324-33. doi: 10.3390/molecules15064324, PMID 20657444.

Masuda Y, Kikuzaki H, Hisamoto M, Nakatani N. Antioxidant properties of gingerol-related compounds from ginger. BioFactors. 2004;21(1-4):293-6. doi: 10.1002/biof.552210157, PMID 15630214.

Ahmed RS, Seth V, Pasha ST, Banerjee BD. Influence of dietary ginger (Zingiber officinales Rosc) on oxidative stress induced by malathion in rats [Zingiber officinales Rosc]. Food Chem Toxicol. 2000;38(5):443-50. doi: 10.1016/s0278-6915(00)00019-3, PMID 10762730.

Murugesan S, Venkateswaran MR, Jayabal S, Periyasamy S. Evaluation of the antioxidant and anti-arthritic potential of Zingiber officinale Rosc. by in vitro and in silico analysis. S Afr J Bot. 2020;130:45-53. doi: 10.1016/j.sajb.2019.12.019.

Kikuzaki H, Nakatani N. Antioxidant effects of some ginger constituents. J Food Sci. 1993;58(6):1407-10. doi: 10.1111/j.1365-2621.1993.tb06194.x.

Ji AM, Al-Hussainy Za AM. Formulation and evaluation of effervescent granules of ibuprofen. Int J Appl Pharm. 2019;11:66-9.

Budiman A, Aulifa DL. A study comparing the antibacterial activity of Ageratum conyzoides L. extract and Piper betle L. extract in gel dosage forms against Staphylococcus aureus. Pharmacogn J. 2020;12(3):473-7. doi: 10.5530/pj.2020.12.73.

Shah RB, Tawakkul MA, Khan MA. Comparative evaluation of flow for pharmaceutical powders and granules. AAPS PharmSciTech. 2008;9(1):250-8. doi: 10.1208/s12249-008-9046-8, PMID 18446489.

Senthil P, Suresh Kumar CH, Raju N, Mohideen S. Formulation and evaluation of gastric oral floating tablet of glipzide. Int J Biol Pharm Res. 2010;1:108-13.

Sandhya S, Gowthami G, Vinod KR, VidyaSravanthi E, Saikumar P, Rao Knv. Formulation and evaluation of herbal effervescent granules incorporated with Limnophila indica extract for bacillary dysentery. Ann Bio Res. 2012;3:63-72.

Aulifa DL, Caroline M, Tristiyanti D, Budiman A. Formulation of the serum gel containing green coffee bean (Coffea robusta L.) extract as an antioxidant and tyrosinase enzyme inhibitor. Rasayan Journal of Chemistry 2020;13:2346-51. doi: 10.31788/RJC.2020.1345866

Budiman A, Khaira N, Aulifa DL. Peel-off gel formulation from black mulberries (Morus nigra L.) leaves extract as a tyrosinase inhibitor. Int J Drug Deliv Technol. 2019;9:525-9.

Rice Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996;20(7):933-56. doi: 10.1016/0891-5849(95)02227-9, PMID 8743980.

Iwansyah AC, Damanik RM, Kustiyah LI, Hanafi M. Relationship between antioxidant properties and nutritional composition of some galactopoietic herbs used in indonesia: a comparative study. Int J Pharm Pharm Sci. 2016;8(12):236-43. doi: 10.22159/ijpps.2016v8i12.14964.

Amic D, Davidovic Amic D, Beslo D, Trinajstic N. Structure-radical scavenging activity relationships of flavonoids. Croat Chem Acta. 2003;76:55-61.

Alía M, Horcajo C, Bravo L, Goya L. Effect of grape antioxidant dietary fiber on the total antioxidant capacity and the activity of liver antioxidant enzymes in rats. Nutr Res. 2003;23(9):1251-67. doi: 10.1016/S0271-5317(03)00131-3.

Wibowo DP, Mariani R, Hasanah SU, Aulifa DL. The chemical composition, antibacterial activity and mechanism of action essential oil of red ginger (Zingiber officinale var. Rubra species from Bogor West Java Indonesia. Int J Pharmacol Res. 2019;11:1210-5.

Soliman KA, Ibrahim HK, Ghorab MM. Formulation of risperidone as self-nano emulsifying drug delivery system in the form of effervescent tablets. J Dispers Sci Technol. 2012;33(8):1127-33. doi: 10.1080/01932691.2011.599235.

Zheng X, Wu F, Hong Y, Shen L, Lin X, Feng Y. Improvements in sticking, hygroscopicity, and compactibility of effervescent systems by fluid-bed coating. RSC Adv. 2019;9(54):31594-608. doi: 10.1039/C9RA05884B.

Nguyen VT. Effect of binder and sweeteners on the production of effervescent artichoke (Cynara scolymus L.) tea tablets. J Food Process Preserv. 2013;37(6):1078-83. doi: 10.1111/j.1745-4549.2012.00808.x.

Shirsand SB, Suresh S, Jodhana LS, Swamy PV. Formulation design and optimization of fast disintegrating lorazepam tablets by an effervescent method. Indian J Pharm Sci. 2010;72(4):431-6. doi: 10.4103/0250-474X.73911, PMID 21218052.

The United States Pharmacopeia (USP). NF28 Convention Inc. USA: United States Pharmacopeia; 2010.

Published

07-01-2022

How to Cite

AULIFA, D. L., WIBOWO, D. P., SAFITRI, N., & BUDIMAN, A. (2022). FORMULATION OF EFFERVESCENT GRANULES FROM RED GINGER (ZINGIBERIS OFFICINALE ROSCOE VAR. RUBRUM) EXTRACT AND ITS ANTIOXIDANT ACTIVITY. International Journal of Applied Pharmaceutics, 14(1), 112–115. https://doi.org/10.22159/ijap.2022v14i1.43377

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